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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     390
XP_006713721                                                           20     390
XP_006718987                                                           17    4312
XP_005273744                                                           16    5631
XP_006716478                                                           16    5631
NP_689485                                                              16    5631
NP_055926                                                              16    7355
NP_001265249                                                           16    7355
NP_001094379                                                           16    7355
NP_001012417                                                           16    7355
XP_005267752                                                           16    7355
XP_005269019                                                           16    7355
XP_006714897                                                           16    7355
NP_057572                                                              16    7355
NP_001137259                                                           16    7355
NP_001135780                                                           15    9605

>NP_006209
          Length = 1068

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  ISVTQEAER 9
          +SVTQEAER
Sbjct: 71 VSVTQEAER 79



>XP_006713721
          Length = 1068

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  ISVTQEAER 9
          +SVTQEAER
Sbjct: 71 VSVTQEAER 79



>XP_006718987
          Length = 401

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  ISVTQEAER 9
          +SVTQE  R
Sbjct: 15 VSVTQEGRR 23



>XP_005273744
          Length = 343

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SVTQEAER 9
           S++QEAER
Sbjct: 147 SLSQEAER 154



>XP_006716478
          Length = 429

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SVTQEAER 9
           S++QEAER
Sbjct: 257 SLSQEAER 264



>NP_689485
          Length = 453

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SVTQEAER 9
           S++QEAER
Sbjct: 257 SLSQEAER 264



>NP_055926
          Length = 544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VTQEAER 9
           VTQ+AER
Sbjct: 308 VTQKAER 314



>NP_001265249
          Length = 93

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  SVTQEAE 8
          SVT+EAE
Sbjct: 84 SVTEEAE 90



>NP_001094379
          Length = 112

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SVTQEAE 8
           SVT+EAE
Sbjct: 103 SVTEEAE 109



>NP_001012417
          Length = 152

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SVTQEAE 8
           SVT+EAE
Sbjct: 143 SVTEEAE 149



>XP_005267752
          Length = 993

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISVTQEAER 9
           ++ T+EAER
Sbjct: 943 VAATKEAER 951



>XP_005269019
          Length = 409

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TQEAER 9
           TQEAER
Sbjct: 368 TQEAER 373



>XP_006714897
          Length = 544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VTQEAER 9
           VTQ+AER
Sbjct: 308 VTQKAER 314



>NP_057572
          Length = 458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TQEAER 9
           TQEAER
Sbjct: 417 TQEAER 422



>NP_001137259
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TQEAER 9
           TQEAER
Sbjct: 393 TQEAER 398



>NP_001135780
          Length = 2156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISVTQEAE 8
           ISV+QE E
Sbjct: 629 ISVSQECE 636



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.112    0.249 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 392,718
Number of extensions: 337
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)