Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              18    1482
NP_005913                                                              18    1482
XP_005267046                                                           18    1482
XP_005267045                                                           18    1482
NP_056493                                                              17    4312
XP_006719869                                                           17    4312
XP_006719868                                                           17    4312
XP_005266408                                                           17    4312
XP_005266407                                                           17    4312
XP_005266403                                                           17    4312
XP_006719867                                                           17    4312
XP_006719866                                                           17    4312
XP_005266405                                                           17    4312
XP_005266404                                                           17    4312
NP_001274103                                                           16    7355
NP_000343                                                              16    7355
NP_004332                                                              16    7355
NP_001106205                                                           16    7355
NP_075559                                                              16    7355
NP_001159484                                                           16    7355
NP_001275640                                                           16    7355
XP_006722435                                                           16    7355
XP_005258240                                                           16    7355
XP_006722434                                                           16    7355
XP_006718349                                                           16    7355
XP_005264615                                                           16    7355
XP_006712164                                                           16    7355
XP_005264614                                                           16    7355
XP_005264613                                                           16    7355
XP_005264612                                                           16    7355
NP_001135389                                                           16    7355
NP_110421                                                              15    9605
NP_001139542                                                           15    9605
NP_001139543                                                           15    9605
NP_001269588                                                           15    9605
NP_060414                                                              15    9605
XP_005266617                                                           15    9605
XP_005266615                                                           15    9605
XP_005266614                                                           15    9605
XP_005266613                                                           15    9605
XP_005253054                                                           15    9605
>NP_006715
          Length = 1558

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            ISVDT ELM
Sbjct: 1311 ISVDTGELM 1319
>NP_005913
          Length = 1608

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            ISVDT ELM
Sbjct: 1361 ISVDTGELM 1369
>XP_005267046
          Length = 1554

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            ISVDT ELM
Sbjct: 1307 ISVDTGELM 1315
>XP_005267045
          Length = 1604

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            ISVDT ELM
Sbjct: 1357 ISVDTGELM 1365
>NP_056493
          Length = 2946

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            +S DTR+L+
Sbjct: 1020 VSTDTRDLL 1028
>XP_006719869
          Length = 2943

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            +S DTR+L+
Sbjct: 1020 VSTDTRDLL 1028
>XP_006719868
          Length = 2938

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            +S DTR+L+
Sbjct: 1020 VSTDTRDLL 1028
>XP_005266408
          Length = 1934

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            +S DTR+L+
Sbjct: 1020 VSTDTRDLL 1028
>XP_005266407
          Length = 2641

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISVDTRELM 9
           +S DTR+L+
Sbjct: 694 VSTDTRDLL 702
>XP_005266403
          Length = 2941

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            +S DTR+L+
Sbjct: 1020 VSTDTRDLL 1028
>XP_006719867
          Length = 2961

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            +S DTR+L+
Sbjct: 1020 VSTDTRDLL 1028
>XP_006719866
          Length = 2962

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            +S DTR+L+
Sbjct: 1020 VSTDTRDLL 1028
>XP_005266405
          Length = 2964

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            +S DTR+L+
Sbjct: 1020 VSTDTRDLL 1028
>XP_005266404
          Length = 2967

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISVDTRELM 9
            +S DTR+L+
Sbjct: 1020 VSTDTRDLL 1028
>NP_001274103
          Length = 1582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ISVDTRELM 9
           +++DT +LM
Sbjct: 421 VAIDTNQLM 429
>NP_000343
          Length = 1581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ISVDTRELM 9
           +++DT +LM
Sbjct: 421 VAIDTNQLM 429
>NP_004332
          Length = 2225

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VDTREL 8
           VDTREL
Sbjct: 119 VDTREL 124
>NP_001106205
          Length = 494

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SVDTRE 7
           SVDTRE
Sbjct: 293 SVDTRE 298
>NP_075559
          Length = 545

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISVDTREL 8
           +S+D REL
Sbjct: 154 LSIDAREL 161
>NP_001159484
          Length = 494

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SVDTRE 7
           SVDTRE
Sbjct: 293 SVDTRE 298
>NP_001275640
          Length = 307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SVDTRE 7
           SVDTRE
Sbjct: 106 SVDTRE 111
>XP_006722435
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SVDTRE 7
           SVDTRE
Sbjct: 150 SVDTRE 155
>XP_005258240
          Length = 494

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SVDTRE 7
           SVDTRE
Sbjct: 293 SVDTRE 298
>XP_006722434
          Length = 494

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SVDTRE 7
           SVDTRE
Sbjct: 293 SVDTRE 298
>XP_006718349
          Length = 1646

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ISVDTRELM 9
           +++DT +LM
Sbjct: 420 VAIDTNQLM 428
>XP_005264615
          Length = 2162

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VDTREL 8
           VDTREL
Sbjct: 119 VDTREL 124
>XP_006712164
          Length = 2179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VDTREL 8
           VDTREL
Sbjct: 119 VDTREL 124
>XP_005264614
          Length = 2191

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VDTREL 8
           VDTREL
Sbjct: 119 VDTREL 124
>XP_005264613
          Length = 2208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VDTREL 8
           VDTREL
Sbjct: 119 VDTREL 124
>XP_005264612
          Length = 2242

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VDTREL 8
           VDTREL
Sbjct: 119 VDTREL 124
>NP_001135389
          Length = 1335

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ISVDTRELM 9
           ISV TREL+
Sbjct: 574 ISVLTRELL 582
>NP_110421
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISVDTREL 8
           + VD+REL
Sbjct: 102 VQVDSREL 109
>NP_001139542
          Length = 744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISVDTREL 8
           + VD+REL
Sbjct: 195 VQVDSREL 202
>NP_001139543
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISVDTREL 8
           + VD+REL
Sbjct: 102 VQVDSREL 109
>NP_001269588
          Length = 1355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVDTREL 8
           SVD REL
Sbjct: 900 SVDNREL 906
>NP_060414
          Length = 1287

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVDTREL 8
           SVD REL
Sbjct: 832 SVDNREL 838
>XP_005266617
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISVDTREL 8
           + VD+REL
Sbjct: 102 VQVDSREL 109
>XP_005266615
          Length = 744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISVDTREL 8
           + VD+REL
Sbjct: 195 VQVDSREL 202
>XP_005266614
          Length = 808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISVDTREL 8
           + VD+REL
Sbjct: 195 VQVDSREL 202
>XP_005266613
          Length = 809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISVDTREL 8
           + VD+REL
Sbjct: 195 VQVDSREL 202
>XP_005253054
          Length = 1375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVDTREL 8
           SVD REL
Sbjct: 920 SVDNREL 926
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.133    0.324 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 447,549
Number of extensions: 616
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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