Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              18    1482
NP_434701                                                              17    4312
NP_434700                                                              17    4312
NP_060224                                                              17    4312
XP_005249256                                                           17    4312
XP_006715189                                                           17    4312
XP_006715188                                                           17    4312
NP_001191800                                                           16    5631
NP_004818                                                              16    7355
NP_001244315                                                           16    7355
NP_001025046                                                           16    7355
NP_079143                                                              16    7355
NP_001157968                                                           16    7355
XP_005248559                                                           16    7355
XP_006714677                                                           16    7355
XP_006714564                                                           16    7355
XP_006714563                                                           16    7355
XP_005248434                                                           16    7355
XP_005248433                                                           16    7355
XP_005263413                                                           16    7355
XP_005263412                                                           16    7355
XP_005263411                                                           16    7355
XP_005247674                                                           16    7355
XP_006713762                                                           16    7355
XP_006713761                                                           16    7355
XP_006713760                                                           16    7355
XP_006713759                                                           16    7355
NP_001276976                                                           15    9605
NP_002843                                                              15    9605
NP_001276975                                                           15    9605
NP_065852                                                              15    9605
NP_057287                                                              15    9605
NP_055796                                                              15    9605
NP_077747                                                              15    9605
NP_001035723                                                           15    9605
XP_006724141                                                           15    9605
XP_006719294                                                           15    9605
XP_005250066                                                           15    9605
XP_005250064                                                           15    9605
XP_005250063                                                           15    9605
XP_005250062                                                           15    9605
XP_005250061                                                           15    9605
XP_006716084                                                           15    9605
XP_005250060                                                           15    9605
XP_005250059                                                           15    9605
NP_001138947                                                           15    9605
NP_116037                                                              15    9605
>NP_004351
          Length = 882

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTRAELHR 9
           ISTRAEL R
Sbjct: 542 ISTRAELDR 550
>NP_434701
          Length = 492

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISTRAELH 8
           I+TR ELH
Sbjct: 361 IATREELH 368
>NP_434700
          Length = 536

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISTRAELH 8
           I+TR ELH
Sbjct: 361 IATREELH 368
>NP_060224
          Length = 1440

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAELHR
Sbjct: 599 RAELHR 604
>XP_005249256
          Length = 1054

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAELHR
Sbjct: 213 RAELHR 218
>XP_006715189
          Length = 1240

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAELHR
Sbjct: 599 RAELHR 604
>XP_006715188
          Length = 1400

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAELHR
Sbjct: 599 RAELHR 604
>NP_001191800
          Length = 110

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TRAELHR 9
           TRA LHR
Sbjct: 102 TRAALHR 108
>NP_004818
          Length = 655

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TRAELHR 9
           T+AELH+
Sbjct: 345 TKAELHQ 351
>NP_001244315
          Length = 611

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TRAELHR 9
           T+AELH+
Sbjct: 345 TKAELHQ 351
>NP_001025046
          Length = 257

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  STRAELHR 9
          S RAE+H+
Sbjct: 83 SLRAEMHK 90
>NP_079143
          Length = 1822

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TRAELHR 9
            T+AELH+
Sbjct: 1101 TKAELHQ 1107
>NP_001157968
          Length = 1854

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 987 RAEMHR 992
>XP_005248559
          Length = 227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  STRAELHR 9
          S RAE+H+
Sbjct: 83 SLRAEMHK 90
>XP_006714677
          Length = 204

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  STRAELHR 9
          S RAE+H+
Sbjct: 83 SLRAEMHK 90
>XP_006714564
          Length = 1537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TRAELHR 9
            T+AELH+
Sbjct: 1101 TKAELHQ 1107
>XP_006714563
          Length = 1766

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TRAELHR 9
            T+AELH+
Sbjct: 1044 TKAELHQ 1050
>XP_005248434
          Length = 1818

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TRAELHR 9
            T+AELH+
Sbjct: 1096 TKAELHQ 1102
>XP_005248433
          Length = 1823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TRAELHR 9
            T+AELH+
Sbjct: 1101 TKAELHQ 1107
>XP_005263413
          Length = 653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TRAELHR 9
           T+AELH+
Sbjct: 343 TKAELHQ 349
>XP_005263412
          Length = 655

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TRAELHR 9
           T+AELH+
Sbjct: 345 TKAELHQ 351
>XP_005263411
          Length = 655

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TRAELHR 9
           T+AELH+
Sbjct: 345 TKAELHQ 351
>XP_005247674
          Length = 1089

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 222 RAEMHR 227
>XP_006713762
          Length = 1197

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RAELHR 9
            RAE+HR
Sbjct: 1031 RAEMHR 1036
>XP_006713761
          Length = 1554

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 687 RAEMHR 692
>XP_006713760
          Length = 1847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 980 RAEMHR 985
>XP_006713759
          Length = 1898

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RAELHR 9
            RAE+HR
Sbjct: 1031 RAEMHR 1036
>NP_001276976
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TRAELH 8
           T+AELH
Sbjct: 297 TKAELH 302
>NP_002843
          Length = 381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TRAELH 8
           TRA+LH
Sbjct: 159 TRADLH 164
>NP_001276975
          Length = 1300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TRAELH 8
           T+AELH
Sbjct: 134 TKAELH 139
>NP_065852
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           +AELHR
Sbjct: 139 KAELHR 144
>NP_057287
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 526 RAEVHR 531
>NP_055796
          Length = 1946

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    RAELHR 9
            +AELHR
Sbjct: 1366 KAELHR 1371
>NP_077747
          Length = 328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 285 RAEVHR 290
>NP_001035723
          Length = 525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 482 RAEVHR 487
>XP_006724141
          Length = 1426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TRAELH 8
           T+AELH
Sbjct: 259 TKAELH 264
>XP_006719294
          Length = 2348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  RAELHR 9
          RA+LHR
Sbjct: 56 RAQLHR 61
>XP_005250066
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 265 RAEVHR 270
>XP_005250064
          Length = 444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 401 RAEVHR 406
>XP_005250063
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 402 RAEVHR 407
>XP_005250062
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 525 RAEVHR 530
>XP_005250061
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 482 RAEVHR 487
>XP_006716084
          Length = 615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 522 RAEVHR 527
>XP_005250060
          Length = 618

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 525 RAEVHR 530
>XP_005250059
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RAE+HR
Sbjct: 526 RAEVHR 531
>NP_001138947
          Length = 2351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  RAELHR 9
          RA+LHR
Sbjct: 56 RAQLHR 61
>NP_116037
          Length = 163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAELHR 9
           RA+LHR
Sbjct: 105 RAQLHR 110
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.128    0.341 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 470,839
Number of extensions: 569
Number of successful extensions: 47
Number of sequences better than 10000.0: 47
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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