Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001121619                                                           18    1482
NP_000258                                                              18    1482
NP_001035957                                                           18    1482
XP_006721991                                                           18    1482
XP_006721990                                                           18    1482
XP_006721989                                                           18    1482
XP_005258041                                                           18    1482
XP_006721988                                                           18    1482
XP_006721987                                                           18    1482
XP_006721986                                                           18    1482
XP_005258040                                                           18    1482
XP_006721985                                                           18    1482
NP_878918                                                              18    1935
NP_055995                                                              18    1935
XP_006720147                                                           18    1935
XP_005267517                                                           18    1935
XP_005267516                                                           18    1935
XP_005267515                                                           18    1935
XP_005267514                                                           18    1935
XP_005267513                                                           18    1935
XP_005267512                                                           18    1935
XP_005267511                                                           18    1935
NP_001036167                                                           17    4312
XP_005246686                                                           17    4312
XP_006712617                                                           17    4312
XP_005246685                                                           17    4312
XP_005246684                                                           17    4312
XP_005246683                                                           17    4312
NP_009200                                                              17    4312
NP_000128                                                              16    5631
NP_775922                                                              16    5631
NP_775837                                                              15    9605
NP_001269109                                                           15    9605
NP_689725                                                              15    9605
NP_006237                                                              15    9605
NP_001269111                                                           15    9605
NP_001269110                                                           15    9605
NP_001269108                                                           15    9605
NP_078801                                                              15    9605
XP_006724284                                                           15    9605
XP_006724283                                                           15    9605
XP_006724282                                                           15    9605
XP_005261537                                                           15    9605
XP_006724215                                                           15    9605
XP_006724214                                                           15    9605
XP_006724213                                                           15    9605
XP_006724212                                                           15    9605
XP_005264439                                                           15    9605
XP_005264438                                                           15    9605
XP_006711877                                                           15    9605
XP_005273321                                                           15    9605
XP_005273318                                                           15    9605
XP_005273317                                                           15    9605
NP_060197                                                              15    9605
>NP_001121619
          Length = 593

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 157 ISTRLQELT 165
>NP_000258
          Length = 2818

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 157 ISTRLQELT 165
>NP_001035957
          Length = 2839

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 157 ISTRLQELT 165
>XP_006721991
          Length = 1632

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 157 ISTRLQELT 165
>XP_006721990
          Length = 2823

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 157 ISTRLQELT 165
>XP_006721989
          Length = 2823

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 157 ISTRLQELT 165
>XP_005258041
          Length = 2836

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 157 ISTRLQELT 165
>XP_006721988
          Length = 2846

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 157 ISTRLQELT 165
>XP_006721987
          Length = 2849

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 157 ISTRLQELT 165
>XP_006721986
          Length = 2854

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 144 ISTRLQELT 152
>XP_005258040
          Length = 2857

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 157 ISTRLQELT 165
>XP_006721985
          Length = 2867

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           IST LQELT
Sbjct: 157 ISTRLQELT 165
>NP_878918
          Length = 6907

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISTMLQELT 9
            IST LQELT
Sbjct: 2891 ISTHLQELT 2899
>NP_055995
          Length = 6885

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISTMLQELT 9
            IST LQELT
Sbjct: 2891 ISTHLQELT 2899
>XP_006720147
          Length = 4788

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISTMLQELT 9
            IST LQELT
Sbjct: 2891 ISTHLQELT 2899
>XP_005267517
          Length = 6883

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISTMLQELT 9
            IST LQELT
Sbjct: 2891 ISTHLQELT 2899
>XP_005267516
          Length = 6884

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISTMLQELT 9
            IST LQELT
Sbjct: 2891 ISTHLQELT 2899
>XP_005267515
          Length = 6899

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISTMLQELT 9
            IST LQELT
Sbjct: 2891 ISTHLQELT 2899
>XP_005267514
          Length = 6908

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISTMLQELT 9
            IST LQELT
Sbjct: 2891 ISTHLQELT 2899
>XP_005267513
          Length = 6921

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISTMLQELT 9
            IST LQELT
Sbjct: 2891 ISTHLQELT 2899
>XP_005267512
          Length = 6922

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISTMLQELT 9
            IST LQELT
Sbjct: 2891 ISTHLQELT 2899
>XP_005267511
          Length = 6922

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISTMLQELT 9
            IST LQELT
Sbjct: 2891 ISTHLQELT 2899
>NP_001036167
          Length = 352

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISTMLQELT 9
           +ST+L+E+T
Sbjct: 126 VSTLLKEIT 134
>XP_005246686
          Length = 349

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISTMLQELT 9
           +ST+L+E+T
Sbjct: 123 VSTLLKEIT 131
>XP_006712617
          Length = 352

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISTMLQELT 9
           +ST+L+E+T
Sbjct: 126 VSTLLKEIT 134
>XP_005246685
          Length = 354

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISTMLQELT 9
           +ST+L+E+T
Sbjct: 161 VSTLLKEIT 169
>XP_005246684
          Length = 355

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISTMLQELT 9
           +ST+L+E+T
Sbjct: 129 VSTLLKEIT 137
>XP_005246683
          Length = 387

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISTMLQELT 9
           +ST+L+E+T
Sbjct: 161 VSTLLKEIT 169
>NP_009200
          Length = 592

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISTMLQELT 9
           +ST+L ELT
Sbjct: 245 VSTVLHELT 253
>NP_000128
          Length = 419

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TMLQELT 9
           TMLQ+LT
Sbjct: 325 TMLQQLT 331
>NP_775922
          Length = 6996

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    ISTMLQELT 9
            IST+ QE+T
Sbjct: 1252 ISTVAQEIT 1260
>NP_775837
          Length = 2151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    STMLQELT 9
            STM+ E+T
Sbjct: 1441 STMINEIT 1448
>NP_001269109
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   STMLQELT 9
           STM  ELT
Sbjct: 419 STMFDELT 426
>NP_689725
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ISTMLQEL 8
           +ST++QEL
Sbjct: 566 LSTVIQEL 573
>NP_006237
          Length = 467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   STMLQELT 9
           STM  ELT
Sbjct: 419 STMFDELT 426
>NP_001269111
          Length = 391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   STMLQELT 9
           STM  ELT
Sbjct: 343 STMFDELT 350
>NP_001269110
          Length = 391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   STMLQELT 9
           STM  ELT
Sbjct: 343 STMFDELT 350
>NP_001269108
          Length = 467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   STMLQELT 9
           STM  ELT
Sbjct: 419 STMFDELT 426
>NP_078801
          Length = 860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   STMLQE 7
           STMLQE
Sbjct: 167 STMLQE 172
>XP_006724284
          Length = 1989

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    STMLQELT 9
            STM+ E+T
Sbjct: 1279 STMINEIT 1286
>XP_006724283
          Length = 2151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    STMLQELT 9
            STM+ E+T
Sbjct: 1441 STMINEIT 1448
>XP_006724282
          Length = 2151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    STMLQELT 9
            STM+ E+T
Sbjct: 1441 STMINEIT 1448
>XP_005261537
          Length = 2151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    STMLQELT 9
            STM+ E+T
Sbjct: 1441 STMINEIT 1448
>XP_006724215
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ISTMLQEL 8
           +ST++QEL
Sbjct: 435 LSTVIQEL 442
>XP_006724214
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ISTMLQEL 8
           +ST++QEL
Sbjct: 453 LSTVIQEL 460
>XP_006724213
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ISTMLQEL 8
           +ST++QEL
Sbjct: 513 LSTVIQEL 520
>XP_006724212
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ISTMLQEL 8
           +ST++QEL
Sbjct: 566 LSTVIQEL 573
>XP_005264439
          Length = 715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   TMLQEL 8
           TMLQEL
Sbjct: 203 TMLQEL 208
>XP_005264438
          Length = 753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   TMLQEL 8
           TMLQEL
Sbjct: 203 TMLQEL 208
>XP_006711877
          Length = 781

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   STMLQE 7
           STMLQE
Sbjct: 167 STMLQE 172
>XP_005273321
          Length = 784

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   STMLQE 7
           STMLQE
Sbjct: 167 STMLQE 172
>XP_005273318
          Length = 829

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   STMLQE 7
           STMLQE
Sbjct: 167 STMLQE 172
>XP_005273317
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   STMLQE 7
           STMLQE
Sbjct: 167 STMLQE 172
>NP_060197
          Length = 689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   TMLQEL 8
           TMLQEL
Sbjct: 203 TMLQEL 208
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.123    0.279 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 465,620
Number of extensions: 589
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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