Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_001243372                                                           17    4312
NP_001243371                                                           17    4312
NP_660282                                                              17    4312
NP_001124320                                                           17    4312
NP_004901                                                              17    4312
XP_006721069                                                           17    4312
NP_006826                                                              16    5631
NP_001243125                                                           16    5631
NP_001645                                                              16    5631
NP_001438                                                              16    5631
XP_006724592                                                           16    5631
XP_006714824                                                           16    5631
NP_659422                                                              16    5631
NP_071923                                                              16    5631
NP_055918                                                              16    7355
NP_001363                                                              16    7355
XP_006721528                                                           16    7355
NP_004653                                                              16    7355
NP_002871                                                              15    9605
NP_878908                                                              15    9605
NP_001035089                                                           15    9605
NP_001398                                                              15    9605
NP_001138295                                                           15    9605
NP_005839                                                              15    9605
NP_001193937                                                           15    9605
NP_055969                                                              15    9605
NP_005413                                                              15    9605
XP_006726924                                                           15    9605
XP_005254179                                                           15    9605
XP_005254178                                                           15    9605
XP_006720435                                                           15    9605
XP_005254177                                                           15    9605
XP_006720434                                                           15    9605
XP_005254176                                                           15    9605
XP_005271710                                                           15    9605
XP_006715557                                                           15    9605
XP_006715556                                                           15    9605
XP_006715555                                                           15    9605
XP_006715554                                                           15    9605
XP_006715553                                                           15    9605
XP_006715552                                                           15    9605
XP_005267054                                                           15    9605
XP_005267053                                                           15    9605
XP_006715551                                                           15    9605
XP_006715550                                                           15    9605
XP_006715549                                                           15    9605
XP_006715548                                                           15    9605
XP_006715547                                                           15    9605
XP_006715546                                                           15    9605
XP_005265417                                                           15    9605
XP_005265416                                                           15    9605
XP_005265415                                                           15    9605
XP_005265414                                                           15    9605
XP_005265412                                                           15    9605
NP_061755                                                              15    9605
>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   ISTAAPVD 8
           ISTAAPVD
Sbjct: 474 ISTAAPVD 481
>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   ISTAAPVD 8
           ISTAAPVD
Sbjct: 269 ISTAAPVD 276
>NP_001243372
          Length = 299

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  STAAPVD 8
          +TAAPVD
Sbjct: 54 TTAAPVD 60
>NP_001243371
          Length = 394

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  STAAPVD 8
          +TAAPVD
Sbjct: 54 TTAAPVD 60
>NP_660282
          Length = 340

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  STAAPVD 8
          +TAAPVD
Sbjct: 54 TTAAPVD 60
>NP_001124320
          Length = 1243

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    AAPVDF 9
            AAPVDF
Sbjct: 1194 AAPVDF 1199
>NP_004901
          Length = 1244

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    AAPVDF 9
            AAPVDF
Sbjct: 1195 AAPVDF 1200
>XP_006721069
          Length = 340

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  STAAPVD 8
          +TAAPVD
Sbjct: 54 TTAAPVD 60
>NP_006826
          Length = 377

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ISTAAPVDF 9
           + TA P+DF
Sbjct: 142 LHTATPLDF 150
>NP_001243125
          Length = 609

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISTAAPVD 8
           +ST AP+D
Sbjct: 224 VSTTAPMD 231
>NP_001645
          Length = 606

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISTAAPVD 8
           +ST AP+D
Sbjct: 221 VSTTAPMD 228
>NP_001438
          Length = 4349

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISTAAPVDF 9
           IST+ P+D+
Sbjct: 514 ISTSKPMDY 522
>XP_006724592
          Length = 611

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISTAAPVD 8
           +ST AP+D
Sbjct: 226 VSTTAPMD 233
>XP_006714824
          Length = 4349

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISTAAPVDF 9
           IST+ P+D+
Sbjct: 514 ISTSKPMDY 522
>NP_659422
          Length = 781

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISTAAPVD 8
           I TAAP+D
Sbjct: 431 IHTAAPLD 438
>NP_071923
          Length = 819

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISTAAPVD 8
           I TAAP+D
Sbjct: 431 IHTAAPLD 438
>NP_055918
          Length = 1925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    STAAPVDF 9
            + +APVDF
Sbjct: 1132 NASAPVDF 1139
>NP_001363
          Length = 4486

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TAAPVDF 9
            TA+PV+F
Sbjct: 3786 TASPVEF 3792
>XP_006721528
          Length = 3869

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TAAPVDF 9
            TA+PV+F
Sbjct: 3786 TASPVEF 3792
>NP_004653
          Length = 798

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TAAPVDF 9
           TA+PV+F
Sbjct: 98  TASPVEF 104
>NP_002871
          Length = 648

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ISTAAPVD 8
           +ST  PVD
Sbjct: 266 VSTTLPVD 273
>NP_878908
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   STAAPV 7
           STAAPV
Sbjct: 326 STAAPV 331
>NP_001035089
          Length = 1651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 268 LSTTDPADF 276
>NP_001398
          Length = 3312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           I   AP+DF
Sbjct: 602 IQVVAPLDF 610
>NP_001138295
          Length = 1906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISTAAPVD 8
            IST+ P+D
Sbjct: 1631 ISTSGPLD 1638
>NP_005839
          Length = 1863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISTAAPVD 8
            IST+ P+D
Sbjct: 1588 ISTSGPLD 1595
>NP_001193937
          Length = 1743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 267 LSTTDPADF 275
>NP_055969
          Length = 234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  AAPVDF 9
          +APVDF
Sbjct: 88 SAPVDF 93
>NP_005413
          Length = 2155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           ISTA   DF
Sbjct: 425 ISTAVETDF 433
>XP_006726924
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   STAAPV 7
           STAAPV
Sbjct: 312 STAAPV 317
>XP_005254179
          Length = 1173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISTAAPVD 8
           IST+ P+D
Sbjct: 898 ISTSGPLD 905
>XP_005254178
          Length = 1863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISTAAPVD 8
            IST+ P+D
Sbjct: 1588 ISTSGPLD 1595
>XP_006720435
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISTAAPVD 8
            IST+ P+D
Sbjct: 1632 ISTSGPLD 1639
>XP_005254177
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISTAAPVD 8
            IST+ P+D
Sbjct: 1632 ISTSGPLD 1639
>XP_006720434
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISTAAPVD 8
            IST+ P+D
Sbjct: 1632 ISTSGPLD 1639
>XP_005254176
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISTAAPVD 8
            IST+ P+D
Sbjct: 1632 ISTSGPLD 1639
>XP_005271710
          Length = 2150

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           ISTA   DF
Sbjct: 425 ISTAVETDF 433
>XP_006715557
          Length = 1744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 267 LSTTDPADF 275
>XP_006715556
          Length = 1051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 269 LSTTDPADF 277
>XP_006715555
          Length = 1638

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 269 LSTTDPADF 277
>XP_006715554
          Length = 1768

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 269 LSTTDPADF 277
>XP_006715553
          Length = 1808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 267 LSTTDPADF 275
>XP_006715552
          Length = 1817

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 269 LSTTDPADF 277
>XP_005267054
          Length = 1823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 269 LSTTDPADF 277
>XP_005267053
          Length = 1824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 268 LSTTDPADF 276
>XP_006715551
          Length = 1825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 269 LSTTDPADF 277
>XP_006715550
          Length = 1830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 269 LSTTDPADF 277
>XP_006715549
          Length = 1830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 267 LSTTDPADF 275
>XP_006715548
          Length = 1831

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 268 LSTTDPADF 276
>XP_006715547
          Length = 1831

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 268 LSTTDPADF 276
>XP_006715546
          Length = 1832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   ISTAAPVDF 9
           +ST  P DF
Sbjct: 269 LSTTDPADF 277
>XP_005265417
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ISTAAPVD 8
           +ST  PVD
Sbjct: 266 VSTTLPVD 273
>XP_005265416
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ISTAAPVD 8
           +ST  PVD
Sbjct: 152 VSTTLPVD 159
>XP_005265415
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ISTAAPVD 8
           +ST  PVD
Sbjct: 185 VSTTLPVD 192
>XP_005265414
          Length = 615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ISTAAPVD 8
           +ST  PVD
Sbjct: 233 VSTTLPVD 240
>XP_005265412
          Length = 648

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ISTAAPVD 8
           +ST  PVD
Sbjct: 266 VSTTLPVD 273
>NP_061755
          Length = 795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ISTAAPVDF 9
           I+  AP+DF
Sbjct: 301 ITLTAPLDF 309
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.131    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 555,495
Number of extensions: 762
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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