Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001185761                                                           20     390
NP_001185763                                                           20     390
NP_055459                                                              20     390
NP_001002811                                                           20     390
NP_001002812                                                           20     390
XP_006726410                                                           20     390
XP_006711719                                                           20     390
XP_006711717                                                           20     390
XP_006711716                                                           20     390
XP_006711715                                                           20     390
XP_006711714                                                           20     390
XP_006711713                                                           20     390
XP_006711712                                                           20     390
XP_006711711                                                           20     390
XP_006711710                                                           20     390
XP_006711709                                                           20     390
XP_005277503                                                           20     390
XP_005273038                                                           20     390
XP_006711708                                                           20     390
XP_005277500                                                           20     390
XP_006711707                                                           20     390
XP_005277499                                                           20     390
XP_006711706                                                           20     390
XP_006711705                                                           20     390
XP_006711164                                                           20     390
XP_003846806                                                           20     390
NP_003946                                                              16    5631
NP_001180401                                                           15    9605
NP_001180400                                                           15    9605
NP_001014986                                                           15    9605
NP_004467                                                              15    9605
NP_060083                                                              15    9605
NP_054781                                                              15    9605
NP_085150                                                              15    9605
XP_006718839                                                           15    9605
>NP_001185761
          Length = 2240

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 593 LSSNEATMQ 601
>NP_001185763
          Length = 2362

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 527 LSSNEATMQ 535
>NP_055459
          Length = 2346

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 527 LSSNEATMQ 535
>NP_001002811
          Length = 1132

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 690 LSSNEATMQ 698
>NP_001002812
          Length = 969

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 527 LSSNEATMQ 535
>XP_006726410
          Length = 2087

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 602 LSSNEATMQ 610
>XP_006711719
          Length = 2302

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 593 LSSNEATMQ 601
>XP_006711717
          Length = 2385

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 593 LSSNEATMQ 601
>XP_006711716
          Length = 2411

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 593 LSSNEATMQ 601
>XP_006711715
          Length = 2428

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 527 LSSNEATMQ 535
>XP_006711714
          Length = 2429

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 527 LSSNEATMQ 535
>XP_006711713
          Length = 2432

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 530 LSSNEATMQ 538
>XP_006711712
          Length = 2473

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 690 LSSNEATMQ 698
>XP_006711711
          Length = 2482

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 690 LSSNEATMQ 698
>XP_006711710
          Length = 2494

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 593 LSSNEATMQ 601
>XP_006711709
          Length = 2495

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 593 LSSNEATMQ 601
>XP_005277503
          Length = 2498

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 690 LSSNEATMQ 698
>XP_005273038
          Length = 2509

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 690 LSSNEATMQ 698
>XP_006711708
          Length = 2512

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 690 LSSNEATMQ 698
>XP_005277500
          Length = 2525

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 690 LSSNEATMQ 698
>XP_006711707
          Length = 2553

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 690 LSSNEATMQ 698
>XP_005277499
          Length = 2561

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 690 LSSNEATMQ 698
>XP_006711706
          Length = 2591

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 690 LSSNEATMQ 698
>XP_006711705
          Length = 2592

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 690 LSSNEATMQ 698
>XP_006711164
          Length = 2087

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ISSNEATMQ 9
           +SSNEATMQ
Sbjct: 602 LSSNEATMQ 610
>XP_003846806
          Length = 136

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  ISSNEATMQ 9
          +SSNEATMQ
Sbjct: 56 LSSNEATMQ 64
>NP_003946
          Length = 225

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISSNEATMQ 9
           +SSN AT+Q
Sbjct: 182 LSSNVATLQ 190
>NP_001180401
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SSNEAT 7
          SSNEAT
Sbjct: 30 SSNEAT 35
>NP_001180400
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SSNEAT 7
          SSNEAT
Sbjct: 30 SSNEAT 35
>NP_001014986
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SSNEAT 7
          SSNEAT
Sbjct: 45 SSNEAT 50
>NP_004467
          Length = 750

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SSNEAT 7
          SSNEAT
Sbjct: 45 SSNEAT 50
>NP_060083
          Length = 566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SNEATMQ 9
           SN+AT+Q
Sbjct: 249 SNDATLQ 255
>NP_054781
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISSNEATMQ 9
           ISS  AT+Q
Sbjct: 378 ISSRSATLQ 386
>NP_085150
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SSNEAT 7
           SSNEAT
Sbjct: 497 SSNEAT 502
>XP_006718839
          Length = 733

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SSNEAT 7
          SSNEAT
Sbjct: 28 SSNEAT 33
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.289   0.0975    0.213 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 318,019
Number of extensions: 422
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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