Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001180360                                                           17    2528
NP_057051                                                              17    2528
NP_036543                                                              17    2528
XP_005270795                                                           17    2528
NP_000213                                                              17    3301
NP_001087241                                                           17    3301
XP_006714101                                                           17    3301
XP_005265799                                                           17    3301
XP_005265798                                                           17    3301
XP_005265797                                                           17    3301
NP_001139669                                                           17    3301
NP_001138619                                                           17    4312
NP_001138618                                                           17    4312
NP_001138620                                                           17    4312
NP_004373                                                              17    4312
NP_705838                                                              15    9605
NP_001008744                                                           15    9605
NP_060789                                                              15    9605
XP_006720261                                                           15    9605
XP_005267905                                                           15    9605
XP_005267904                                                           15    9605
XP_006720260                                                           15    9605

>NP_001180360
          Length = 779

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISSARVNYS 9
           +SSA +NYS
Sbjct: 492 VSSAELNYS 500



>NP_057051
          Length = 807

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISSARVNYS 9
           +SSA +NYS
Sbjct: 492 VSSAELNYS 500



>NP_036543
          Length = 752

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISSARVNYS 9
           +SSA +NYS
Sbjct: 437 VSSAELNYS 445



>XP_005270795
          Length = 783

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISSARVNYS 9
           +SSA +NYS
Sbjct: 468 VSSAELNYS 476



>NP_000213
          Length = 976

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISSARVNYS 9
           ISSARVN S
Sbjct: 277 ISSARVNDS 285



>NP_001087241
          Length = 972

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISSARVNYS 9
           ISSARVN S
Sbjct: 277 ISSARVNDS 285



>XP_006714101
          Length = 526

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISSARVNYS 9
           ISSARVN S
Sbjct: 278 ISSARVNDS 286



>XP_005265799
          Length = 973

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISSARVNYS 9
           ISSARVN S
Sbjct: 278 ISSARVNDS 286



>XP_005265798
          Length = 976

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISSARVNYS 9
           ISSARVN S
Sbjct: 278 ISSARVNDS 286



>XP_005265797
          Length = 977

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISSARVNYS 9
           ISSARVN S
Sbjct: 278 ISSARVNDS 286



>NP_001139669
          Length = 7081

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    ISSARVNYS 9
            ISS ++NYS
Sbjct: 2939 ISSQQINYS 2947



>NP_001138619
          Length = 375

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SARVNYS 9
          +ARVNYS
Sbjct: 54 AARVNYS 60



>NP_001138618
          Length = 444

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SARVNYS 9
           +ARVNYS
Sbjct: 94  AARVNYS 100



>NP_001138620
          Length = 401

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SARVNYS 9
           +ARVNYS
Sbjct: 94  AARVNYS 100



>NP_004373
          Length = 415

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SARVNYS 9
           +ARVNYS
Sbjct: 94  AARVNYS 100



>NP_705838
          Length = 4069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISSARVNYS 9
            +SSA+ NY+
Sbjct: 2885 LSSAKSNYA 2893



>NP_001008744
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISSARVNY 8
           +SSA+ NY
Sbjct: 197 VSSAQFNY 204



>NP_060789
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISSARVNY 8
           +SSA+ NY
Sbjct: 197 VSSAQFNY 204



>XP_006720261
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISSARVNY 8
           +SSA+ NY
Sbjct: 197 VSSAQFNY 204



>XP_005267905
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISSARVNY 8
           +SSA+ NY
Sbjct: 197 VSSAQFNY 204



>XP_005267904
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISSARVNY 8
           +SSA+ NY
Sbjct: 197 VSSAQFNY 204



>XP_006720260
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISSARVNY 8
           +SSA+ NY
Sbjct: 197 VSSAQFNY 204



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.114    0.275 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 344,227
Number of extensions: 389
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)