Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_057613                                                              17    3301
XP_006719899                                                           17    3301
XP_006719898                                                           17    3301
XP_006719897                                                           17    3301
XP_006719895                                                           17    3301
XP_005266476                                                           17    3301
NP_005553                                                              17    4312
NP_061486                                                              17    4312
XP_006711379                                                           17    4312
XP_005245207                                                           17    4312
NP_001269488                                                           16    7355
NP_009003                                                              16    7355
NP_001269485                                                           16    7355
NP_001269486                                                           16    7355
NP_001269487                                                           16    7355
XP_005247137                                                           16    7355
NP_000205                                                              15    9605
NP_004095                                                              15    9605
NP_478066                                                              15    9605
NP_003449                                                              15    9605
XP_006724087                                                           15    9605
XP_006713443                                                           15    9605
>NP_057613
          Length = 1188

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  ISSAEYSIL 9
          IS+A+YS+L
Sbjct: 73 ISTAKYSVL 81
>XP_006719899
          Length = 677

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  ISSAEYSIL 9
          IS+A+YS+L
Sbjct: 33 ISTAKYSVL 41
>XP_006719898
          Length = 763

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  ISSAEYSIL 9
          IS+A+YS+L
Sbjct: 33 ISTAKYSVL 41
>XP_006719897
          Length = 1148

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  ISSAEYSIL 9
          IS+A+YS+L
Sbjct: 33 ISTAKYSVL 41
>XP_006719895
          Length = 1082

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  ISSAEYSIL 9
          IS+A+YS+L
Sbjct: 33 ISTAKYSVL 41
>XP_005266476
          Length = 1148

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  ISSAEYSIL 9
          IS+A+YS+L
Sbjct: 33 ISTAKYSVL 41
>NP_005553
          Length = 1193

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SSAEYSI 8
           SSAEYS+
Sbjct: 198 SSAEYSV 204
>NP_061486
          Length = 1111

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SSAEYSI 8
           SSAEYS+
Sbjct: 198 SSAEYSV 204
>XP_006711379
          Length = 1208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SSAEYSI 8
           SSAEYS+
Sbjct: 261 SSAEYSV 267
>XP_005245207
          Length = 1256

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SSAEYSI 8
           SSAEYS+
Sbjct: 261 SSAEYSV 267
>NP_001269488
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISSAEYSIL 9
           ISS EY++L
Sbjct: 255 ISSDEYTLL 263
>NP_009003
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISSAEYSIL 9
           ISS EY++L
Sbjct: 319 ISSDEYTLL 327
>NP_001269485
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISSAEYSIL 9
           ISS EY++L
Sbjct: 319 ISSDEYTLL 327
>NP_001269486
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISSAEYSIL 9
           ISS EY++L
Sbjct: 319 ISSDEYTLL 327
>NP_001269487
          Length = 397

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISSAEYSIL 9
           ISS EY++L
Sbjct: 319 ISSDEYTLL 327
>XP_005247137
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISSAEYSIL 9
           ISS EY++L
Sbjct: 319 ISSDEYTLL 327
>NP_000205
          Length = 1218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SAEYSI 8
           SAEYSI
Sbjct: 993 SAEYSI 998
>NP_004095
          Length = 2511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SSAEYSI 8
           SSAEY++
Sbjct: 730 SSAEYNV 736
>NP_478066
          Length = 235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  ISSAEYSI 8
          +SSA YSI
Sbjct: 37 LSSAAYSI 44
>NP_003449
          Length = 3926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SSAEYS 7
            SSAEYS
Sbjct: 3701 SSAEYS 3706
>XP_006724087
          Length = 234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  ISSAEYSI 8
          +SSA YSI
Sbjct: 37 LSSAAYSI 44
>XP_006713443
          Length = 3970

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SSAEYS 7
            SSAEYS
Sbjct: 3745 SSAEYS 3750
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.122    0.277 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 388,950
Number of extensions: 1382
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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