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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005269596                                                           17    2528
NP_005426                                                              17    4312
NP_055571                                                              17    4312
NP_001008938                                                           17    4312
XP_006726519                                                           17    4312
XP_006716191                                                           17    4312
XP_006716190                                                           17    4312
NP_001360                                                              16    7355
NP_001153699                                                           16    7355
NP_005227                                                              16    7355
NP_079413                                                              16    7355
NP_001273456                                                           16    7355
NP_005384                                                              16    7355
NP_996999                                                              16    7355
NP_996994                                                              16    7355
NP_612152                                                              16    7355
NP_001156729                                                           16    7355
XP_006721418                                                           16    7355
XP_006720764                                                           16    7355
XP_005254752                                                           16    7355
XP_006720763                                                           16    7355
XP_006720762                                                           16    7355
XP_005254751                                                           16    7355
XP_006715811                                                           16    7355
XP_005249842                                                           16    7355
XP_006715715                                                           16    7355
XP_005248319                                                           16    7355
NP_001108225                                                           15    9605
NP_001265067                                                           15    9605
NP_000109                                                              15    9605
NP_115879                                                              15    9605

>XP_005269596
          Length = 388

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISQKMEISC 9
           +SQKM++ C
Sbjct: 374 LSQKMQLHC 382



>NP_005426
          Length = 1597

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    ISQKMEISC 9
            +SQK+E  C
Sbjct: 1371 LSQKIEFEC 1379



>NP_055571
          Length = 1972

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISQKMEISC 9
            IS +ME++C
Sbjct: 1843 ISPQMEVTC 1851



>NP_001008938
          Length = 2032

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISQKMEISC 9
            IS +ME++C
Sbjct: 1903 ISPQMEVTC 1911



>XP_006726519
          Length = 1493

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    ISQKMEISC 9
            +SQK+E  C
Sbjct: 1352 LSQKIEFEC 1360



>XP_006716191
          Length = 1543

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    ISQKMEISC 9
            +SQK+E  C
Sbjct: 1371 LSQKIEFEC 1379



>XP_006716190
          Length = 1597

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    ISQKMEISC 9
            +SQK+E  C
Sbjct: 1371 LSQKIEFEC 1379



>NP_001360
          Length = 4624

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ISQKMEIS 8
            ++QK+EIS
Sbjct: 3795 VTQKLEIS 3802



>NP_001153699
          Length = 2330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SQKMEISC 9
           + K+E+SC
Sbjct: 351 NSKLEVSC 358



>NP_005227
          Length = 916

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   ISQKMEI 7
           IS+KMEI
Sbjct: 357 ISEKMEI 363



>NP_079413
          Length = 2443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SQKMEISC 9
           + K+E+SC
Sbjct: 351 NSKLEVSC 358



>NP_001273456
          Length = 975

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SQKMEISC 9
           SQK E+ C
Sbjct: 780 SQKTEVCC 787



>NP_005384
          Length = 1909

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1    ISQKMEISC 9
            + QK++++C
Sbjct: 1885 VGQKLQLAC 1893



>NP_996999
          Length = 866

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SQKMEISC 9
           S K+E+SC
Sbjct: 552 SGKLELSC 559



>NP_996994
          Length = 923

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SQKMEISC 9
           S K+E+SC
Sbjct: 609 SGKLELSC 616



>NP_612152
          Length = 2849

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SQKMEISC 9
           S KME SC
Sbjct: 185 SLKMEASC 192



>NP_001156729
          Length = 1932

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1    ISQKMEISC 9
            + QK++++C
Sbjct: 1908 VGQKLQLAC 1916



>XP_006721418
          Length = 972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SQKMEISC 9
           SQK E+ C
Sbjct: 777 SQKTEVCC 784



>XP_006720764
          Length = 1584

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SQKMEISC 9
           + K+E+SC
Sbjct: 351 NSKLEVSC 358



>XP_005254752
          Length = 2357

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SQKMEISC 9
           + K+E+SC
Sbjct: 265 NSKLEVSC 272



>XP_006720763
          Length = 2395

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SQKMEISC 9
           + K+E+SC
Sbjct: 351 NSKLEVSC 358



>XP_006720762
          Length = 2397

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SQKMEISC 9
           + K+E+SC
Sbjct: 351 NSKLEVSC 358



>XP_005254751
          Length = 2407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SQKMEISC 9
           + K+E+SC
Sbjct: 351 NSKLEVSC 358



>XP_006715811
          Length = 488

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SQKMEISC 9
           S K+E+SC
Sbjct: 174 SGKLELSC 181



>XP_005249842
          Length = 923

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SQKMEISC 9
           S K+E+SC
Sbjct: 609 SGKLELSC 616



>XP_006715715
          Length = 2023

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SQKMEISC 9
           S KME SC
Sbjct: 185 SLKMEASC 192



>XP_005248319
          Length = 4609

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ISQKMEIS 8
            ++QK+EIS
Sbjct: 3780 VTQKLEIS 3787



>NP_001108225
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ISQKMEISC 9
           ++ K+E+SC
Sbjct: 234 VTVKVELSC 242



>NP_001265067
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  ISQKMEISC 9
          ++ K+E+SC
Sbjct: 52 VTVKVELSC 60



>NP_000109
          Length = 625

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ISQKMEISC 9
           ++ K+E+SC
Sbjct: 234 VTVKVELSC 242



>NP_115879
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3  QKMEISC 9
          QK+ +SC
Sbjct: 15 QKLRVSC 21



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.124    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 472,049
Number of extensions: 654
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)