Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     390
NP_671714                                                              20     390
XP_006715890                                                           20     390
XP_005250160                                                           20     390
XP_005250159                                                           20     390
XP_006714215                                                           17    4312
XP_006714214                                                           17    4312
XP_005262915                                                           17    4312
NP_055806                                                              17    4312
NP_835235                                                              16    5631
NP_006222                                                              16    7355
NP_005909                                                              16    7355
NP_006501                                                              16    7355
NP_001269332                                                           16    7355
NP_001269333                                                           16    7355
NP_631905                                                              16    7355
NP_775899                                                              16    7355
NP_001136157                                                           15    9605
NP_055530                                                              15    9605
NP_060224                                                              15    9605
NP_001264044                                                           15    9605
NP_004183                                                              15    9605
NP_054872                                                              15    9605
NP_001166945                                                           15    9605
NP_001026786                                                           15    9605
NP_001171922                                                           15    9605
XP_005274840                                                           15    9605
XP_005274491                                                           15    9605
XP_005255329                                                           15    9605
XP_006720940                                                           15    9605
XP_005255327                                                           15    9605
XP_006719916                                                           15    9605
XP_006719915                                                           15    9605
XP_006719914                                                           15    9605
XP_005266530                                                           15    9605
XP_006719913                                                           15    9605
XP_006719912                                                           15    9605
XP_006719911                                                           15    9605
XP_006719910                                                           15    9605
XP_006719909                                                           15    9605
XP_006719010                                                           15    9605
XP_005271793                                                           15    9605
XP_006715189                                                           15    9605
XP_006715188                                                           15    9605
NP_055634                                                              15    9605
NP_112567                                                              15    9605
NP_000686                                                              15    9605
NP_065126                                                              15    9605
>NP_005742
          Length = 3907

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ISQFTEKID 9
            ISQFTEKI+
Sbjct: 2821 ISQFTEKIE 2829
>NP_671714
          Length = 3899

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ISQFTEKID 9
            ISQFTEKI+
Sbjct: 2813 ISQFTEKIE 2821
>XP_006715890
          Length = 3860

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ISQFTEKID 9
            ISQFTEKI+
Sbjct: 2774 ISQFTEKIE 2782
>XP_005250160
          Length = 3939

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ISQFTEKID 9
            ISQFTEKI+
Sbjct: 2853 ISQFTEKIE 2861
>XP_005250159
          Length = 3952

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ISQFTEKID 9
            ISQFTEKI+
Sbjct: 2866 ISQFTEKIE 2874
>XP_006714215
          Length = 3544

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISQFTEKI 8
           +SQF EKI
Sbjct: 445 LSQFAEKI 452
>XP_006714214
          Length = 3544

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISQFTEKI 8
           +SQF EKI
Sbjct: 445 LSQFAEKI 452
>XP_005262915
          Length = 3544

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISQFTEKI 8
           +SQF EKI
Sbjct: 445 LSQFAEKI 452
>NP_055806
          Length = 3526

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISQFTEKI 8
           +SQF EKI
Sbjct: 427 LSQFAEKI 434
>NP_835235
          Length = 505

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISQFTEKI 8
           + QFTEK+
Sbjct: 253 LHQFTEKV 260
>NP_006222
          Length = 2286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  SQFTEKID 9
          SQ+T+K+D
Sbjct: 38 SQWTDKMD 45
>NP_005909
          Length = 338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISQFTEKID 9
           ISQ T K+D
Sbjct: 209 ISQCTPKVD 217
>NP_006501
          Length = 513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISQFTEKI 8
           + QFTEK+
Sbjct: 291 LKQFTEKM 298
>NP_001269332
          Length = 296

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISQFTEKID 9
           ISQ T K+D
Sbjct: 167 ISQCTPKVD 175
>NP_001269333
          Length = 231

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISQFTEKID 9
           ISQ T K+D
Sbjct: 102 ISQCTPKVD 110
>NP_631905
          Length = 381

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   ISQFTEKID 9
           +++FTEK++
Sbjct: 224 VNRFTEKLN 232
>NP_775899
          Length = 4462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISQFTEKID 9
            ++QF E+ID
Sbjct: 1032 LAQFQEQID 1040
>NP_001136157
          Length = 2087

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SQFTEKI 8
           S FTEK+
Sbjct: 881 SSFTEKV 887
>NP_055530
          Length = 1738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SQFTEKI 8
           S FTEK+
Sbjct: 532 SSFTEKV 538
>NP_060224
          Length = 1440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ISQFTEKID 9
           +SQ+ EK D
Sbjct: 310 LSQYFEKFD 318
>NP_001264044
          Length = 4516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    ISQFTEKID 9
            + QF E+ID
Sbjct: 1079 LEQFKEQID 1087
>NP_004183
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SQFTEKID 9
          S+F EK+D
Sbjct: 40 SKFKEKLD 47
>NP_054872
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISQFTEKID 9
          ISQ+TE ++
Sbjct: 63 ISQYTEALN 71
>NP_001166945
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SQFTEKID 9
          S+F EK+D
Sbjct: 40 SKFKEKLD 47
>NP_001026786
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  ISQFTEKI 8
          ISQ TEK+
Sbjct: 61 ISQGTEKV 68
>NP_001171922
          Length = 1619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISQFTEKI 8
            +S+F EKI
Sbjct: 1152 VSEFQEKI 1159
>XP_005274840
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SQFTEKID 9
          S+F EK+D
Sbjct: 40 SKFKEKLD 47
>XP_005274491
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SQFTEKID 9
          S+F EK+D
Sbjct: 40 SKFKEKLD 47
>XP_005255329
          Length = 958

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISQFTEKID 9
          ISQ+TE ++
Sbjct: 63 ISQYTEALN 71
>XP_006720940
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISQFTEKID 9
          ISQ+TE ++
Sbjct: 63 ISQYTEALN 71
>XP_005255327
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISQFTEKID 9
          ISQ+TE ++
Sbjct: 63 ISQYTEALN 71
>XP_006719916
          Length = 1482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISQFTEKI 8
            +S+F EKI
Sbjct: 1015 VSEFQEKI 1022
>XP_006719915
          Length = 1531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISQFTEKI 8
            +S+F EKI
Sbjct: 1064 VSEFQEKI 1071
>XP_006719914
          Length = 1639

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISQFTEKI 8
            +S+F EKI
Sbjct: 1172 VSEFQEKI 1179
>XP_005266530
          Length = 1643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISQFTEKI 8
            +S+F EKI
Sbjct: 1176 VSEFQEKI 1183
>XP_006719913
          Length = 1650

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISQFTEKI 8
            +S+F EKI
Sbjct: 1225 VSEFQEKI 1232
>XP_006719912
          Length = 1672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISQFTEKI 8
            +S+F EKI
Sbjct: 1205 VSEFQEKI 1212
>XP_006719911
          Length = 1688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISQFTEKI 8
            +S+F EKI
Sbjct: 1221 VSEFQEKI 1228
>XP_006719910
          Length = 1692

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISQFTEKI 8
            +S+F EKI
Sbjct: 1225 VSEFQEKI 1232
>XP_006719909
          Length = 1692

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISQFTEKI 8
            +S+F EKI
Sbjct: 1225 VSEFQEKI 1232
>XP_006719010
          Length = 1436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SQFTEKI 8
           S FTEK+
Sbjct: 230 SSFTEKV 236
>XP_005271793
          Length = 2061

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SQFTEKI 8
           S FTEK+
Sbjct: 855 SSFTEKV 861
>XP_006715189
          Length = 1240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ISQFTEKID 9
           +SQ+ EK D
Sbjct: 310 LSQYFEKFD 318
>XP_006715188
          Length = 1400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ISQFTEKID 9
           +SQ+ EK D
Sbjct: 310 LSQYFEKFD 318
>NP_055634
          Length = 708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1 ISQFTEK 7
         +SQ+TEK
Sbjct: 1 MSQYTEK 7
>NP_112567
          Length = 1623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISQFTEKI 8
            +S+F EKI
Sbjct: 1156 VSEFQEKI 1163
>NP_000686
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  ISQFTEKI 8
          ISQ TEK+
Sbjct: 61 ISQGTEKV 68
>NP_065126
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  ISQFTEK 7
          ISQ TEK
Sbjct: 40 ISQLTEK 46
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.133    0.336 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 440,646
Number of extensions: 637
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package