Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003961                                                              17    2528
XP_006716300                                                           17    2528
NP_001138923                                                           17    3301
NP_055621                                                              16    5631
NP_001129471                                                           16    5631
NP_003387                                                              16    5631
XP_006725694                                                           16    5631
XP_005257694                                                           16    5631
NP_000029                                                              16    7355
NP_001120983                                                           16    7355
NP_001120982                                                           16    7355
NP_001040625                                                           16    7355
NP_005854                                                              16    7355
NP_849157                                                              16    7355
NP_059129                                                              16    7355
XP_006714159                                                           16    7355
XP_006714158                                                           16    7355
XP_006714157                                                           16    7355
NP_001229695                                                           15    9605
NP_004737                                                              15    9605
NP_001003809                                                           15    9605
NP_001229693                                                           15    9605
NP_001132914                                                           15    9605
NP_919228                                                              15    9605
NP_001229690                                                           15    9605
NP_055486                                                              15    9605
NP_001229692                                                           15    9605
NP_001229691                                                           15    9605
NP_001229694                                                           15    9605
NP_006461                                                              15    9605
XP_005258233                                                           15    9605
XP_005258232                                                           15    9605
XP_005258231                                                           15    9605
XP_006722430                                                           15    9605
XP_005258230                                                           15    9605
XP_005258229                                                           15    9605
XP_005258228                                                           15    9605
XP_006716845                                                           15    9605
XP_005251541                                                           15    9605
XP_006716843                                                           15    9605
XP_005251540                                                           15    9605
XP_005249622                                                           15    9605
XP_005249621                                                           15    9605
NP_066553                                                              15    9605
>NP_003961
          Length = 1465

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   PFRSVGV 9
           PFRSVG+
Sbjct: 255 PFRSVGL 261
>XP_006716300
          Length = 1445

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   PFRSVGV 9
           PFRSVG+
Sbjct: 255 PFRSVGL 261
>NP_001138923
          Length = 870

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           SPFRS+ +
Sbjct: 140 SPFRSINI 147
>NP_055621
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SPFRSVG 8
           SP RSVG
Sbjct: 781 SPIRSVG 787
>NP_001129471
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SPFRSVG 8
           SP RSVG
Sbjct: 781 SPIRSVG 787
>NP_003387
          Length = 357

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ISPFRSVG 8
           +SPFR  G
Sbjct: 228 LSPFRETG 235
>XP_006725694
          Length = 329

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ISPFRSVG 8
           +SPFR  G
Sbjct: 228 LSPFRETG 235
>XP_005257694
          Length = 329

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ISPFRSVG 8
           +SPFR  G
Sbjct: 228 LSPFRETG 235
>NP_000029
          Length = 2843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISPFRSVG 8
            +SP+RS G
Sbjct: 1099 VSPYRSRG 1106
>NP_001120983
          Length = 2825

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISPFRSVG 8
            +SP+RS G
Sbjct: 1081 VSPYRSRG 1088
>NP_001120982
          Length = 2843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ISPFRSVG 8
            +SP+RS G
Sbjct: 1099 VSPYRSRG 1106
>NP_001040625
          Length = 596

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISPFRSVG 8
           I+PF+S G
Sbjct: 500 IAPFQSAG 507
>NP_005854
          Length = 542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISPFRSVG 8
           I+PF+S G
Sbjct: 446 IAPFQSAG 453
>NP_849157
          Length = 1077

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISPFRSVG 8
           ISP R+VG
Sbjct: 688 ISPSRNVG 695
>NP_059129
          Length = 1616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   ISPFRSVGV 9
           ++PF+S+G+
Sbjct: 378 LNPFQSLGL 386
>XP_006714159
          Length = 1077

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISPFRSVG 8
           ISP R+VG
Sbjct: 688 ISPSRNVG 695
>XP_006714158
          Length = 1077

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISPFRSVG 8
           ISP R+VG
Sbjct: 688 ISPSRNVG 695
>XP_006714157
          Length = 1077

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISPFRSVG 8
           ISP R+VG
Sbjct: 688 ISPSRNVG 695
>NP_001229695
          Length = 685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 380 SKFQSVGV 387
>NP_004737
          Length = 977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 672 SKFQSVGV 679
>NP_001003809
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 370 SKFQSVGV 377
>NP_001229693
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 378 SKFQSVGV 385
>NP_001132914
          Length = 800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SPFRSVGV 9
          SP RS+G+
Sbjct: 16 SPMRSLGL 23
>NP_919228
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SPFRSVGV 9
          SP RS+G+
Sbjct: 16 SPMRSLGL 23
>NP_001229690
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 672 SKFQSVGV 679
>NP_055486
          Length = 1083

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISPFRSVGV 9
           I+ F+S+GV
Sbjct: 573 ITAFQSIGV 581
>NP_001229692
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 394 SKFQSVGV 401
>NP_001229691
          Length = 661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 356 SKFQSVGV 363
>NP_001229694
          Length = 627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 370 SKFQSVGV 377
>NP_006461
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   PFRSVGV 9
           PFR +GV
Sbjct: 491 PFRRLGV 497
>XP_005258233
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 367 SKFQSVGV 374
>XP_005258232
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 388 SKFQSVGV 395
>XP_005258231
          Length = 695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 390 SKFQSVGV 397
>XP_006722430
          Length = 731

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 426 SKFQSVGV 433
>XP_005258230
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 432 SKFQSVGV 439
>XP_005258229
          Length = 987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 682 SKFQSVGV 689
>XP_005258228
          Length = 987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SPFRSVGV 9
           S F+SVGV
Sbjct: 682 SKFQSVGV 689
>XP_006716845
          Length = 546

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   ISPFRS 6
           +SPFRS
Sbjct: 185 LSPFRS 190
>XP_005251541
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   ISPFRS 6
           +SPFRS
Sbjct: 185 LSPFRS 190
>XP_006716843
          Length = 924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   ISPFRS 6
           +SPFRS
Sbjct: 185 LSPFRS 190
>XP_005251540
          Length = 934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   ISPFRS 6
           +SPFRS
Sbjct: 185 LSPFRS 190
>XP_005249622
          Length = 1021

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISPFRSVGV 9
           I+ F+S+GV
Sbjct: 573 ITAFQSIGV 581
>XP_005249621
          Length = 1058

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISPFRSVGV 9
           I+ F+S+GV
Sbjct: 548 ITAFQSIGV 556
>NP_066553
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ISPFRSVG 8
           +SPFR  G
Sbjct: 129 LSPFRKQG 136
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.145    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 609,781
Number of extensions: 851
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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