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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     509
XP_005262424                                                           20     509
NP_002227                                                              17    3301
NP_001006658                                                           16    5631
NP_065830                                                              16    5631
NP_055994                                                              16    7355
NP_001138586                                                           16    7355
NP_001271266                                                           16    7355
XP_006723519                                                           16    7355
XP_006723518                                                           16    7355
XP_006723517                                                           16    7355
NP_072047                                                              15    9605
NP_001276103                                                           15    9605
NP_001276105                                                           15    9605
NP_001276104                                                           15    9605
NP_001259017                                                           15    9605
NP_001259016                                                           15    9605
NP_005062                                                              15    9605
XP_006722907                                                           15    9605
XP_006722906                                                           15    9605
XP_006722905                                                           15    9605

>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISMTLTHVM 9
            ISM LTHVM
Sbjct: 2506 ISMNLTHVM 2514



>XP_005262424
          Length = 2875

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISMTLTHVM 9
            ISM LTHVM
Sbjct: 2301 ISMNLTHVM 2309



>NP_002227
          Length = 494

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ISMTLTHV 8
           +S+TLTH+
Sbjct: 268 VSLTLTHL 275



>NP_001006658
          Length = 1181

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISMTLTHVM 9
           ++MT THV+
Sbjct: 434 VAMTKTHVI 442



>NP_065830
          Length = 1170

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISMTLTHVM 9
           ++MT THV+
Sbjct: 423 VAMTKTHVI 431



>NP_055994
          Length = 1297

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MTLTHVM 9
           MTL+HV+
Sbjct: 334 MTLSHVL 340



>NP_001138586
          Length = 1236

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MTLTHVM 9
           MTL+HV+
Sbjct: 273 MTLSHVL 279



>NP_001271266
          Length = 1197

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MTLTHVM 9
           MTL+HV+
Sbjct: 234 MTLSHVL 240



>XP_006723519
          Length = 223

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ISMTLTH 7
           +SMT TH
Sbjct: 184 LSMTFTH 190



>XP_006723518
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ISMTLTH 7
           +SMT TH
Sbjct: 238 LSMTFTH 244



>XP_006723517
          Length = 327

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ISMTLTH 7
           +SMT TH
Sbjct: 288 LSMTFTH 294



>NP_072047
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISMTLTHV 8
           +SMTL H+
Sbjct: 423 LSMTLKHL 430



>NP_001276103
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISMTLTHV 8
           +SMTL H+
Sbjct: 356 LSMTLKHL 363



>NP_001276105
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISMTLTHV 8
           +SMTL H+
Sbjct: 234 LSMTLKHL 241



>NP_001276104
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ISMTLTHV 8
           +SMTL H+
Sbjct: 298 LSMTLKHL 305



>NP_001259017
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SMTLTHVM 9
          +MTL HV+
Sbjct: 43 TMTLEHVL 50



>NP_001259016
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SMTLTHVM 9
          +MTL HV+
Sbjct: 43 TMTLEHVL 50



>NP_005062
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SMTLTHVM 9
          +MTL HV+
Sbjct: 43 TMTLEHVL 50



>XP_006722907
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SMTLTHVM 9
          +MTL HV+
Sbjct: 43 TMTLEHVL 50



>XP_006722906
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SMTLTHVM 9
          +MTL HV+
Sbjct: 43 TMTLEHVL 50



>XP_006722905
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SMTLTHVM 9
          +MTL HV+
Sbjct: 43 TMTLEHVL 50



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.127    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 491,443
Number of extensions: 441
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)