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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_663791                                                              16    5631
NP_663783                                                              16    5631
NP_001497                                                              16    7355
XP_006711403                                                           16    7355
NP_653300                                                              16    7355
NP_056110                                                              15    9605
NP_778203                                                              15    9605
NP_008850                                                              15    9605
NP_056968                                                              15    9605
NP_002965                                                              15    9605
XP_005258159                                                           15    9605
XP_006722384                                                           15    9605

>NP_663791
          Length = 192

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SLTSALAV 9
           SLT+ALAV
Sbjct: 163 SLTAALAV 170



>NP_663783
          Length = 191

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SLTSALAV 9
           SLT+ALAV
Sbjct: 162 SLTAALAV 169



>NP_001497
          Length = 356

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   SLTSALA 8
           SLTSALA
Sbjct: 330 SLTSALA 336



>XP_006711403
          Length = 961

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SLTSALAV 9
           SLTS LAV
Sbjct: 212 SLTSGLAV 219



>NP_653300
          Length = 1029

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SLTSALAV 9
           SLTS LAV
Sbjct: 280 SLTSGLAV 287



>NP_056110
          Length = 2005

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    ISLTSALA 8
            I++TSALA
Sbjct: 1081 INITSALA 1088



>NP_778203
          Length = 1007

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ISLTSALAV 9
          IS+T+ LAV
Sbjct: 13 ISITTCLAV 21



>NP_008850
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ISLTSALAV 9
          IS+TSAL +
Sbjct: 32 ISITSALGM 40



>NP_056968
          Length = 519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISLTSALAV 9
           ISL+ ALA+
Sbjct: 449 ISLSGALAI 457



>NP_002965
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ISLTSALAV 9
          IS+TSAL +
Sbjct: 32 ISITSALGM 40



>XP_005258159
          Length = 1817

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   ISLTSALA 8
           I++TSALA
Sbjct: 893 INITSALA 900



>XP_006722384
          Length = 1877

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   ISLTSALA 8
           I++TSALA
Sbjct: 953 INITSALA 960



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.121    0.260 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 200,609
Number of extensions: 156
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)