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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003855                                                              18    1482
NP_733751                                                              18    1935
NP_001124535                                                           18    1935
NP_001124534                                                           18    1935
NP_078908                                                              18    1935
XP_006716142                                                           18    1935
XP_005250088                                                           18    1935
XP_006716141                                                           18    1935
XP_005250085                                                           18    1935
XP_006716140                                                           18    1935
XP_005250084                                                           18    1935
XP_005250083                                                           18    1935
XP_005250082                                                           18    1935
NP_001277403                                                           15    9605
NP_055622                                                              15    9605
NP_001273387                                                           15    9605
NP_775105                                                              15    9605
NP_001073996                                                           15    9605
NP_001036148                                                           15    9605
NP_076404                                                              15    9605
NP_003461                                                              15    9605
NP_001273386                                                           15    9605
XP_006719011                                                           15    9605
XP_006715506                                                           15    9605
XP_006715505                                                           15    9605
XP_006715504                                                           15    9605
XP_006715503                                                           15    9605
XP_006715502                                                           15    9605
XP_005263028                                                           15    9605
XP_006712602                                                           15    9605
NP_001088                                                              15    9605

>NP_003855
          Length = 220

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  SFSRRIQK 9
          SFS+RIQK
Sbjct: 84 SFSKRIQK 91



>NP_733751
          Length = 4911

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISFSRRIQK 9
            IS SRRIQK
Sbjct: 4870 ISSSRRIQK 4878



>NP_001124535
          Length = 137

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SFSRRIQK 9
          SFS+R+QK
Sbjct: 46 SFSKRVQK 53



>NP_001124534
          Length = 142

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SFSRRIQK 9
          SFS+R+QK
Sbjct: 46 SFSKRVQK 53



>NP_078908
          Length = 183

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SFSRRIQK 9
          SFS+R+QK
Sbjct: 46 SFSKRVQK 53



>XP_006716142
          Length = 4927

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISFSRRIQK 9
            IS SRRIQK
Sbjct: 4886 ISSSRRIQK 4894



>XP_005250088
          Length = 4928

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISFSRRIQK 9
            IS SRRIQK
Sbjct: 4887 ISSSRRIQK 4895



>XP_006716141
          Length = 4959

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISFSRRIQK 9
            IS SRRIQK
Sbjct: 4918 ISSSRRIQK 4926



>XP_005250085
          Length = 4979

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISFSRRIQK 9
            IS SRRIQK
Sbjct: 4938 ISSSRRIQK 4946



>XP_006716140
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISFSRRIQK 9
            IS SRRIQK
Sbjct: 4941 ISSSRRIQK 4949



>XP_005250084
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISFSRRIQK 9
            IS SRRIQK
Sbjct: 4941 ISSSRRIQK 4949



>XP_005250083
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISFSRRIQK 9
            IS SRRIQK
Sbjct: 4941 ISSSRRIQK 4949



>XP_005250082
          Length = 4983

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISFSRRIQK 9
            IS SRRIQK
Sbjct: 4942 ISSSRRIQK 4950



>NP_001277403
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SFSRRIQK 9
           SFSRR+ K
Sbjct: 681 SFSRRLIK 688



>NP_055622
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SFSRRIQK 9
           SFSRR+ K
Sbjct: 682 SFSRRLIK 689



>NP_001273387
          Length = 1003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SFSRRI 7
          SFSRRI
Sbjct: 50 SFSRRI 55



>NP_775105
          Length = 1659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISFSRRIQK 9
          +SFS+ I+K
Sbjct: 77 VSFSKPIEK 85



>NP_001073996
          Length = 2116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    SRRIQK 9
            SRRIQK
Sbjct: 1768 SRRIQK 1773



>NP_001036148
          Length = 1683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISFSRRIQK 9
          +SFS+ I+K
Sbjct: 77 VSFSKPIEK 85



>NP_076404
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SFSRRIQK 9
           SFSRR+ K
Sbjct: 320 SFSRRLFK 327



>NP_003461
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SFSRRI 7
           SFSRRI
Sbjct: 149 SFSRRI 154



>NP_001273386
          Length = 1086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SFSRRI 7
           SFSRRI
Sbjct: 133 SFSRRI 138



>XP_006719011
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SFSRRIQK 9
           SFSRR+ K
Sbjct: 645 SFSRRLIK 652



>XP_006715506
          Length = 1507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISFSRRIQK 9
          +SFS+ I+K
Sbjct: 77 VSFSKPIEK 85



>XP_006715505
          Length = 1673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISFSRRIQK 9
          +SFS+ I+K
Sbjct: 77 VSFSKPIEK 85



>XP_006715504
          Length = 1683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISFSRRIQK 9
          +SFS+ I+K
Sbjct: 77 VSFSKPIEK 85



>XP_006715503
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISFSRRIQK 9
          +SFS+ I+K
Sbjct: 77 VSFSKPIEK 85



>XP_006715502
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISFSRRIQK 9
          +SFS+ I+K
Sbjct: 77 VSFSKPIEK 85



>XP_005263028
          Length = 152

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   ISFSRR 6
           ISFSRR
Sbjct: 130 ISFSRR 135



>XP_006712602
          Length = 2142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    SRRIQK 9
            SRRIQK
Sbjct: 1794 SRRIQK 1799



>NP_001088
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   ISFSRRIQK 9
           +SF++R+Q+
Sbjct: 382 LSFAKRLQQ 390



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.140    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 410,828
Number of extensions: 490
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)