Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              17    3301
NP_006429                                                              17    3301
XP_005250813                                                           17    3301
XP_006716142                                                           17    3301
XP_005250088                                                           17    3301
XP_006716141                                                           17    3301
XP_005250085                                                           17    3301
XP_006716140                                                           17    3301
XP_005250084                                                           17    3301
XP_005250083                                                           17    3301
XP_005250082                                                           17    3301
NP_005493                                                              17    4312
NP_001128726                                                           17    4312
XP_005251837                                                           17    4312
XP_005251835                                                           17    4312
XP_006717058                                                           17    4312
XP_005251833                                                           17    4312
XP_005251831                                                           17    4312
XP_005251830                                                           17    4312
XP_005245941                                                           17    4312
NP_659414                                                              16    5631
NP_001182144                                                           16    5631
XP_005244990                                                           16    5631
XP_005244988                                                           16    5631
XP_006711256                                                           16    5631
XP_006711255                                                           16    5631
NP_000615                                                              15    9605
NP_689646                                                              15    9605
NP_001010870                                                           15    9605
NP_940841                                                              15    9605
NP_001161831                                                           15    9605
NP_001003845                                                           15    9605
XP_006718830                                                           15    9605
XP_005246599                                                           15    9605
>NP_733751
          Length = 4911

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISDYLAKSV 9
            ISD LAKSV
Sbjct: 1448 ISDDLAKSV 1456
>NP_006429
          Length = 277

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   ISDYLAKS 8
           I+DY+AKS
Sbjct: 195 IADYVAKS 202
>XP_005250813
          Length = 208

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   ISDYLAKS 8
           I+DY+AKS
Sbjct: 126 IADYVAKS 133
>XP_006716142
          Length = 4927

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISDYLAKSV 9
            ISD LAKSV
Sbjct: 1449 ISDDLAKSV 1457
>XP_005250088
          Length = 4928

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISDYLAKSV 9
            ISD LAKSV
Sbjct: 1449 ISDDLAKSV 1457
>XP_006716141
          Length = 4959

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISDYLAKSV 9
            ISD LAKSV
Sbjct: 1449 ISDDLAKSV 1457
>XP_005250085
          Length = 4979

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISDYLAKSV 9
            ISD LAKSV
Sbjct: 1449 ISDDLAKSV 1457
>XP_006716140
          Length = 4982

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISDYLAKSV 9
            ISD LAKSV
Sbjct: 1449 ISDDLAKSV 1457
>XP_005250084
          Length = 4982

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISDYLAKSV 9
            ISD LAKSV
Sbjct: 1449 ISDDLAKSV 1457
>XP_005250083
          Length = 4982

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISDYLAKSV 9
            ISD LAKSV
Sbjct: 1448 ISDDLAKSV 1456
>XP_005250082
          Length = 4983

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISDYLAKSV 9
            ISD LAKSV
Sbjct: 1449 ISDDLAKSV 1457
>NP_005493
          Length = 2261

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    ISDYLAKS 8
            ISDYL K+
Sbjct: 1505 ISDYLVKT 1512
>NP_001128726
          Length = 505

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   DYLAKSV 9
           DYLAK+V
Sbjct: 471 DYLAKNV 477
>XP_005251837
          Length = 1659

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    ISDYLAKS 8
            ISDYL K+
Sbjct: 1507 ISDYLVKT 1514
>XP_005251835
          Length = 2074

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    ISDYLAKS 8
            ISDYL K+
Sbjct: 1507 ISDYLVKT 1514
>XP_006717058
          Length = 2100

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    ISDYLAKS 8
            ISDYL K+
Sbjct: 1507 ISDYLVKT 1514
>XP_005251833
          Length = 2203

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    ISDYLAKS 8
            ISDYL K+
Sbjct: 1447 ISDYLVKT 1454
>XP_005251831
          Length = 2263

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    ISDYLAKS 8
            ISDYL K+
Sbjct: 1507 ISDYLVKT 1514
>XP_005251830
          Length = 2263

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    ISDYLAKS 8
            ISDYL K+
Sbjct: 1507 ISDYLVKT 1514
>XP_005245941
          Length = 503

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   DYLAKSV 9
           DYLAK+V
Sbjct: 469 DYLAKNV 475
>NP_659414
          Length = 426

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISDYLAKSV 9
           ++DYLAK +
Sbjct: 114 LADYLAKEL 122
>NP_001182144
          Length = 775

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISDYLAKSV 9
           ++DYLAK +
Sbjct: 114 LADYLAKEL 122
>XP_005244990
          Length = 396

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISDYLAKSV 9
           ++DYLAK +
Sbjct: 104 LADYLAKEL 112
>XP_005244988
          Length = 755

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISDYLAKSV 9
           ++DYLAK +
Sbjct: 114 LADYLAKEL 122
>XP_006711256
          Length = 765

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISDYLAKSV 9
           ++DYLAK +
Sbjct: 104 LADYLAKEL 112
>XP_006711255
          Length = 765

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISDYLAKSV 9
           ++DYLAK +
Sbjct: 104 LADYLAKEL 112
>NP_000615
          Length = 406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   ISDYLAK 7
           I+DY+AK
Sbjct: 172 INDYVAK 178
>NP_689646
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SDYLAKSV 9
           SD+L KS+
Sbjct: 142 SDFLIKSI 149
>NP_001010870
          Length = 2096

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SDYLAKSV 9
            ++YL+KSV
Sbjct: 1192 TEYLSKSV 1199
>NP_940841
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SDYLAKSV 9
           SD+L KS+
Sbjct: 142 SDFLIKSI 149
>NP_001161831
          Length = 2066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SDYLAKSV 9
            ++YL+KSV
Sbjct: 1192 TEYLSKSV 1199
>NP_001003845
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SDYLAKSV 9
           SD+LAK V
Sbjct: 368 SDHLAKHV 375
>XP_006718830
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SDYLAKSV 9
           SD+L KS+
Sbjct: 142 SDFLIKSI 149
>XP_005246599
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SDYLAKSV 9
           SD+LAK V
Sbjct: 412 SDHLAKHV 419
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.128    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 391,102
Number of extensions: 232
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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