Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_036462                                                              18    1935
NP_001243397                                                           18    1935
XP_005269721                                                           18    1935
NP_000483                                                              16    7355
NP_005550                                                              16    7355
XP_006715905                                                           16    7355
NP_009225                                                              15    9605
NP_009230                                                              15    9605
NP_009228                                                              15    9605
NP_009229                                                              15    9605
NP_009231                                                              15    9605
XP_006722104                                                           15    9605
XP_006722103                                                           15    9605
XP_006722102                                                           15    9605
XP_006722101                                                           15    9605
XP_006722100                                                           15    9605
XP_006722099                                                           15    9605
XP_006722098                                                           15    9605
XP_006722097                                                           15    9605
XP_006722096                                                           15    9605
XP_006722095                                                           15    9605
XP_006722094                                                           15    9605
XP_006722093                                                           15    9605
XP_006722092                                                           15    9605
>NP_036462
          Length = 2073

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISASTRKVN 9
           ISAST KVN
Sbjct: 420 ISASTLKVN 428
>NP_001243397
          Length = 1890

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISASTRKVN 9
           ISAST KVN
Sbjct: 420 ISASTLKVN 428
>XP_005269721
          Length = 2073

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISASTRKVN 9
           ISAST KVN
Sbjct: 420 ISASTLKVN 428
>NP_000483
          Length = 1480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SASTRKVN 9
           +ASTRKV+
Sbjct: 788 TASTRKVS 795
>NP_005550
          Length = 3075

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    ASTRKVN 9
            AST+KVN
Sbjct: 1638 ASTQKVN 1644
>XP_006715905
          Length = 1588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SASTRKVN 9
           +ASTRKV+
Sbjct: 896 TASTRKVS 903
>NP_009225
          Length = 1863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISASTRKVN 9
            ++AST +VN
Sbjct: 1639 LTASTERVN 1647
>NP_009230
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISASTRKVN 9
           ++AST +VN
Sbjct: 535 LTASTERVN 543
>NP_009228
          Length = 1816

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISASTRKVN 9
            ++AST +VN
Sbjct: 1592 LTASTERVN 1600
>NP_009229
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISASTRKVN 9
           ++AST +VN
Sbjct: 535 LTASTERVN 543
>NP_009231
          Length = 1884

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISASTRKVN 9
            ++AST +VN
Sbjct: 1660 LTASTERVN 1668
>XP_006722104
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISASTRKVN 9
           ++AST +VN
Sbjct: 494 LTASTERVN 502
>XP_006722103
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISASTRKVN 9
           ++AST +VN
Sbjct: 535 LTASTERVN 543
>XP_006722102
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISASTRKVN 9
           ++AST +VN
Sbjct: 535 LTASTERVN 543
>XP_006722101
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISASTRKVN 9
           ++AST +VN
Sbjct: 535 LTASTERVN 543
>XP_006722100
          Length = 760

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISASTRKVN 9
           ++AST +VN
Sbjct: 536 LTASTERVN 544
>XP_006722099
          Length = 1736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISASTRKVN 9
            ++AST +VN
Sbjct: 1512 LTASTERVN 1520
>XP_006722098
          Length = 1793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISASTRKVN 9
            ++AST +VN
Sbjct: 1569 LTASTERVN 1577
>XP_006722097
          Length = 1862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISASTRKVN 9
            ++AST +VN
Sbjct: 1638 LTASTERVN 1646
>XP_006722096
          Length = 1862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISASTRKVN 9
            ++AST +VN
Sbjct: 1638 LTASTERVN 1646
>XP_006722095
          Length = 1862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISASTRKVN 9
            ++AST +VN
Sbjct: 1638 LTASTERVN 1646
>XP_006722094
          Length = 1863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISASTRKVN 9
            ++AST +VN
Sbjct: 1639 LTASTERVN 1647
>XP_006722093
          Length = 1863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISASTRKVN 9
            ++AST +VN
Sbjct: 1639 LTASTERVN 1647
>XP_006722092
          Length = 1863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ISASTRKVN 9
            ++AST +VN
Sbjct: 1639 LTASTERVN 1647
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.109    0.249 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 274,133
Number of extensions: 295
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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