Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003310                                                              17    3301
NP_597676                                                              17    3301
NP_001243779                                                           17    3301
NP_596869                                                              17    3301
XP_005246888                                                           17    3301
XP_006712788                                                           17    3301
XP_006712787                                                           17    3301
XP_006712786                                                           17    3301
NP_001254479                                                           17    3301
NP_597681                                                              17    3301
NP_005742                                                              17    4312
NP_671714                                                              17    4312
XP_006715890                                                           17    4312
XP_005250160                                                           17    4312
XP_005250159                                                           17    4312
NP_006653                                                              16    5631
NP_848622                                                              16    7355
NP_001245141                                                           16    7355
NP_002109                                                              16    7355
NP_659425                                                              15    9605

>NP_003310
          Length = 26926

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    ISALTLGIS 9
            I A+TLG+S
Sbjct: 9874 IKAMTLGVS 9882



>NP_597676
          Length = 27051

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     ISALTLGIS 9
             I A+TLG+S
Sbjct: 9999  IKAMTLGVS 10007



>NP_001243779
          Length = 34350

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     ISALTLGIS 9
             I A+TLG+S
Sbjct: 17298 IKAMTLGVS 17306



>NP_596869
          Length = 33423

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     ISALTLGIS 9
             I A+TLG+S
Sbjct: 16371 IKAMTLGVS 16379



>XP_005246888
          Length = 22663

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    ISALTLGIS 9
            I A+TLG+S
Sbjct: 5611 IKAMTLGVS 5619



>XP_006712788
          Length = 24022

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    ISALTLGIS 9
            I A+TLG+S
Sbjct: 6970 IKAMTLGVS 6978



>XP_006712787
          Length = 33978

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     ISALTLGIS 9
             I A+TLG+S
Sbjct: 16926 IKAMTLGVS 16934



>XP_006712786
          Length = 35484

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     ISALTLGIS 9
             I A+TLG+S
Sbjct: 18432 IKAMTLGVS 18440



>NP_001254479
          Length = 35991

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     ISALTLGIS 9
             I A+TLG+S
Sbjct: 18939 IKAMTLGVS 18947



>NP_597681
          Length = 27118

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     ISALTLGIS 9
             I A+TLG+S
Sbjct: 10066 IKAMTLGVS 10074



>NP_005742
          Length = 3907

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISALTLGIS 9
            ISALTL IS
Sbjct: 2601 ISALTLRIS 2609



>NP_671714
          Length = 3899

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISALTLGIS 9
            ISALTL IS
Sbjct: 2593 ISALTLRIS 2601



>XP_006715890
          Length = 3860

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISALTLGIS 9
            ISALTL IS
Sbjct: 2554 ISALTLRIS 2562



>XP_005250160
          Length = 3939

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISALTLGIS 9
            ISALTL IS
Sbjct: 2633 ISALTLRIS 2641



>XP_005250159
          Length = 3952

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ISALTLGIS 9
            ISALTL IS
Sbjct: 2646 ISALTLRIS 2654



>NP_006653
          Length = 3230

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    SALTLGIS 9
            SALTLG++
Sbjct: 1503 SALTLGLA 1510



>NP_848622
          Length = 1124

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISALTLGIS 9
           IS LT+GI+
Sbjct: 413 ISLLTMGIN 421



>NP_001245141
          Length = 453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISALTLGIS 9
           +SAL+LG S
Sbjct: 136 VSALSLGTS 144



>NP_002109
          Length = 263

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ISALTLGI 8
           +SA+TLG+
Sbjct: 221 VSAVTLGL 228



>NP_659425
          Length = 216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ISALTLGI 8
           +SAL LGI
Sbjct: 154 VSALLLGI 161



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.134    0.321 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 335,541
Number of extensions: 274
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)