Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005253405                                                           17    4312
NP_777601                                                              17    4312
NP_055753                                                              16    5631
XP_006719356                                                           16    5631
XP_006719355                                                           16    5631
XP_006719354                                                           16    5631
XP_006719353                                                           16    5631
NP_001245200                                                           16    7355
NP_000240                                                              16    7355
NP_001161089                                                           16    7355
NP_056110                                                              16    7355
NP_002537                                                              16    7355
XP_005258159                                                           16    7355
XP_006722384                                                           16    7355
XP_005265218                                                           16    7355
NP_057289                                                              15    9605
NP_001008895                                                           15    9605
NP_001265442                                                           15    9605
NP_001265443                                                           15    9605
NP_003580                                                              15    9605
NP_005133                                                              15    9605
XP_005273967                                                           15    9605
>XP_005253405
          Length = 1463

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    IRSIFGMLL 9
            + SIFG+LL
Sbjct: 1374 LSSIFGLLL 1382
>NP_777601
          Length = 1453

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    IRSIFGMLL 9
            + SIFG+LL
Sbjct: 1364 LSSIFGLLL 1372
>NP_055753
          Length = 919

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RSIFGMLL 9
           RSIFG +L
Sbjct: 340 RSIFGSML 347
>XP_006719356
          Length = 708

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RSIFGMLL 9
           RSIFG +L
Sbjct: 340 RSIFGSML 347
>XP_006719355
          Length = 920

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RSIFGMLL 9
           RSIFG +L
Sbjct: 340 RSIFGSML 347
>XP_006719354
          Length = 959

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RSIFGMLL 9
           RSIFG +L
Sbjct: 340 RSIFGSML 347
>XP_006719353
          Length = 960

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RSIFGMLL 9
           RSIFG +L
Sbjct: 340 RSIFGSML 347
>NP_001245200
          Length = 687

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   IRSIFG 6
           IRSIFG
Sbjct: 216 IRSIFG 221
>NP_000240
          Length = 756

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   IRSIFG 6
           IRSIFG
Sbjct: 216 IRSIFG 221
>NP_001161089
          Length = 658

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   IRSIFG 6
           IRSIFG
Sbjct: 118 IRSIFG 123
>NP_056110
          Length = 2005

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SIFGMLL 9
            ++FGMLL
Sbjct: 1825 TVFGMLL 1831
>NP_002537
          Length = 401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SIFGMLL 9
           S+FG+LL
Sbjct: 167 SVFGLLL 173
>XP_005258159
          Length = 1817

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SIFGMLL 9
            ++FGMLL
Sbjct: 1637 TVFGMLL 1643
>XP_006722384
          Length = 1877

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SIFGMLL 9
            ++FGMLL
Sbjct: 1697 TVFGMLL 1703
>XP_005265218
          Length = 687

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   IRSIFG 6
           IRSIFG
Sbjct: 216 IRSIFG 221
>NP_057289
          Length = 345

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3 SIFGMLL 9
         S+FG+LL
Sbjct: 2 SLFGLLL 8
>NP_001008895
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IRSIFGML 8
           +RS+ GML
Sbjct: 214 LRSLLGML 221
>NP_001265442
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IRSIFGML 8
           +RS+ GML
Sbjct: 114 LRSLLGML 121
>NP_001265443
          Length = 667

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IRSIFGML 8
           +RS+ GML
Sbjct: 122 LRSLLGML 129
>NP_003580
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IRSIFGML 8
           +RS+ GML
Sbjct: 114 LRSLLGML 121
>NP_005133
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RSIFGMLL 9
           RS+FG +L
Sbjct: 196 RSLFGQVL 203
>XP_005273967
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RSIFGMLL 9
           RS+FG +L
Sbjct: 171 RSLFGQVL 178
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.352    0.166    0.459 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 355,858
Number of extensions: 441
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)

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