Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           20     509
NP_000242                                                              20     509
XP_005264389                                                           20     509
NP_000746                                                              17    4312
NP_001244292                                                           17    4312
XP_005251766                                                           17    4312
XP_005251765                                                           17    4312
XP_005251764                                                           17    4312
XP_005251763                                                           17    4312
XP_005273450                                                           17    4312
XP_005273448                                                           17    4312
NP_116166                                                              16    5631
XP_006716388                                                           16    5631
NP_001002814                                                           16    7355
NP_079427                                                              16    7355
NP_808224                                                              16    7355
NP_057241                                                              16    7355
NP_001034794                                                           16    7355
XP_006724668                                                           16    7355
XP_006724667                                                           16    7355
XP_006724666                                                           16    7355
XP_006724665                                                           16    7355
XP_006724664                                                           16    7355
XP_006724663                                                           16    7355
XP_006724662                                                           16    7355
NP_036214                                                              15    9605
NP_079410                                                              15    9605
NP_056996                                                              15    9605
NP_001275964                                                           15    9605
NP_001275963                                                           15    9605
NP_612352                                                              15    9605
NP_958930                                                              15    9605
NP_958929                                                              15    9605
NP_958931                                                              15    9605
XP_005256233                                                           15    9605
XP_005256232                                                           15    9605
XP_005256231                                                           15    9605
XP_005256229                                                           15    9605
XP_005256228                                                           15    9605
XP_005256227                                                           15    9605
XP_006721346                                                           15    9605
XP_005256226                                                           15    9605
XP_006721345                                                           15    9605
XP_006721344                                                           15    9605
XP_006721343                                                           15    9605
XP_005256225                                                           15    9605
XP_005250985                                                           15    9605
XP_006716634                                                           15    9605
XP_005250907                                                           15    9605
>NP_001245210
          Length = 868

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IRSATKDSL 9
           +RSATKDSL
Sbjct: 670 LRSATKDSL 678
>NP_000242
          Length = 934

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IRSATKDSL 9
           +RSATKDSL
Sbjct: 736 LRSATKDSL 744
>XP_005264389
          Length = 921

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IRSATKDSL 9
           +RSATKDSL
Sbjct: 736 LRSATKDSL 744
>NP_000746
          Length = 626

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IRSATKDSL 9
           IRSA+ DSL
Sbjct: 468 IRSASMDSL 476
>NP_001244292
          Length = 605

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IRSATKDSL 9
           IRSA+ DSL
Sbjct: 447 IRSASMDSL 455
>XP_005251766
          Length = 605

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IRSATKDSL 9
           IRSA+ DSL
Sbjct: 447 IRSASMDSL 455
>XP_005251765
          Length = 605

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IRSATKDSL 9
           IRSA+ DSL
Sbjct: 447 IRSASMDSL 455
>XP_005251764
          Length = 622

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IRSATKDSL 9
           IRSA+ DSL
Sbjct: 464 IRSASMDSL 472
>XP_005251763
          Length = 627

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IRSATKDSL 9
           IRSA+ DSL
Sbjct: 469 IRSASMDSL 477
>XP_005273450
          Length = 264

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IRSATKDSL 9
           +RSA +DSL
Sbjct: 99  VRSACRDSL 107
>XP_005273448
          Length = 451

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IRSATKDSL 9
           +RSA +DSL
Sbjct: 99  VRSACRDSL 107
>NP_116166
          Length = 1338

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    RSATKDSL 9
            RSA++DSL
Sbjct: 1273 RSASRDSL 1280
>XP_006716388
          Length = 1314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    RSATKDSL 9
            RSA++DSL
Sbjct: 1249 RSASRDSL 1256
>NP_001002814
          Length = 1283

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SATKDSL 9
           S+TKDSL
Sbjct: 384 SSTKDSL 390
>NP_079427
          Length = 649

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SATKDSL 9
           S+TKDSL
Sbjct: 384 SSTKDSL 390
>NP_808224
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SATKDSL 9
          +ATKDSL
Sbjct: 50 AATKDSL 56
>NP_057241
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SATKDSL 9
          +ATKDSL
Sbjct: 50 AATKDSL 56
>NP_001034794
          Length = 1431

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SATKDSL 9
          +ATKDSL
Sbjct: 50 AATKDSL 56
>XP_006724668
          Length = 681

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SATKDSL 9
          +ATKDSL
Sbjct: 50 AATKDSL 56
>XP_006724667
          Length = 1387

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SATKDSL 9
          +ATKDSL
Sbjct: 6  AATKDSL 12
>XP_006724666
          Length = 1387

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SATKDSL 9
          +ATKDSL
Sbjct: 6  AATKDSL 12
>XP_006724665
          Length = 1431

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SATKDSL 9
          +ATKDSL
Sbjct: 50 AATKDSL 56
>XP_006724664
          Length = 1431

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SATKDSL 9
          +ATKDSL
Sbjct: 50 AATKDSL 56
>XP_006724663
          Length = 1431

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SATKDSL 9
          +ATKDSL
Sbjct: 50 AATKDSL 56
>XP_006724662
          Length = 1431

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SATKDSL 9
          +ATKDSL
Sbjct: 50 AATKDSL 56
>NP_036214
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SATKDSL 9
           SAT+DSL
Sbjct: 402 SATEDSL 408
>NP_079410
          Length = 2881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SATKDSL 9
            SATKD L
Sbjct: 1458 SATKDEL 1464
>NP_056996
          Length = 1406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IRSATKDS 8
           IRS+ KDS
Sbjct: 654 IRSSLKDS 661
>NP_001275964
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ATKDSL 9
          ATKDSL
Sbjct: 93 ATKDSL 98
>NP_001275963
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ATKDSL 9
           ATKDSL
Sbjct: 170 ATKDSL 175
>NP_612352
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ATKDSL 9
           ATKDSL
Sbjct: 170 ATKDSL 175
>NP_958930
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ATKDSL 9
           ATKDSL
Sbjct: 170 ATKDSL 175
>NP_958929
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ATKDSL 9
           ATKDSL
Sbjct: 170 ATKDSL 175
>NP_958931
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ATKDSL 9
           ATKDSL
Sbjct: 188 ATKDSL 193
>XP_005256233
          Length = 2391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SATKDSL 9
           SATKD L
Sbjct: 951 SATKDEL 957
>XP_005256232
          Length = 2424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SATKDSL 9
           SATKD L
Sbjct: 984 SATKDEL 990
>XP_005256231
          Length = 2573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SATKDSL 9
            SATKD L
Sbjct: 1458 SATKDEL 1464
>XP_005256229
          Length = 2881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SATKDSL 9
            SATKD L
Sbjct: 1458 SATKDEL 1464
>XP_005256228
          Length = 2882

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SATKDSL 9
            SATKD L
Sbjct: 1458 SATKDEL 1464
>XP_005256227
          Length = 2897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SATKDSL 9
            SATKD L
Sbjct: 1458 SATKDEL 1464
>XP_006721346
          Length = 2898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SATKDSL 9
            SATKD L
Sbjct: 1458 SATKDEL 1464
>XP_005256226
          Length = 2898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SATKDSL 9
            SATKD L
Sbjct: 1458 SATKDEL 1464
>XP_006721345
          Length = 2898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SATKDSL 9
            SATKD L
Sbjct: 1458 SATKDEL 1464
>XP_006721344
          Length = 2898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SATKDSL 9
            SATKD L
Sbjct: 1458 SATKDEL 1464
>XP_006721343
          Length = 2898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SATKDSL 9
            SATKD L
Sbjct: 1458 SATKDEL 1464
>XP_005256225
          Length = 2898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    SATKDSL 9
            SATKD L
Sbjct: 1458 SATKDEL 1464
>XP_005250985
          Length = 1406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IRSATKDS 8
           IRS+ KDS
Sbjct: 654 IRSSLKDS 661
>XP_006716634
          Length = 1420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IRSATKDS 8
           IRS+ KDS
Sbjct: 668 IRSSLKDS 675
>XP_005250907
          Length = 1082

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SATKDSL 9
           SAT+DSL
Sbjct: 333 SATEDSL 339
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.118    0.276 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 392,593
Number of extensions: 422
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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