Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     869
XP_006716142                                                           19     869
XP_005250088                                                           19     869
XP_006716141                                                           19     869
XP_005250085                                                           19     869
XP_006716140                                                           19     869
XP_005250084                                                           19     869
XP_005250083                                                           19     869
XP_005250082                                                           19     869
NP_056020                                                              17    2528
NP_115565                                                              17    2528
XP_006720028                                                           17    2528
XP_005268363                                                           17    2528
XP_005268362                                                           17    2528
XP_005268358                                                           17    2528
XP_005268360                                                           17    2528
XP_005268357                                                           17    2528
XP_005268356                                                           17    2528
NP_001035877                                                           17    3301
NP_001035814                                                           17    3301
NP_056062                                                              17    3301
XP_006721215                                                           17    3301
XP_006721214                                                           17    3301
XP_006721213                                                           17    3301
XP_006721212                                                           17    3301
XP_005255869                                                           17    3301
XP_006721211                                                           17    3301
NP_579875                                                              16    7355
NP_758847                                                              16    7355
NP_001349                                                              15    9605
NP_001253969                                                           15    9605
NP_004964                                                              15    9605
NP_066554                                                              15    9605
NP_891554                                                              15    9605
NP_001243404                                                           15    9605
XP_005261676                                                           15    9605
XP_006724320                                                           15    9605
XP_005250695                                                           15    9605
XP_005249146                                                           15    9605
NP_001929                                                              15    9605
NP_115540                                                              15    9605
>NP_733751
          Length = 4911

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IRQRSELFF 9
            IRQRSE FF
Sbjct: 3076 IRQRSEPFF 3084
>XP_006716142
          Length = 4927

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IRQRSELFF 9
            IRQRSE FF
Sbjct: 3093 IRQRSEPFF 3101
>XP_005250088
          Length = 4928

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IRQRSELFF 9
            IRQRSE FF
Sbjct: 3093 IRQRSEPFF 3101
>XP_006716141
          Length = 4959

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IRQRSELFF 9
            IRQRSE FF
Sbjct: 3093 IRQRSEPFF 3101
>XP_005250085
          Length = 4979

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IRQRSELFF 9
            IRQRSE FF
Sbjct: 3093 IRQRSEPFF 3101
>XP_006716140
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IRQRSELFF 9
            IRQRSE FF
Sbjct: 3093 IRQRSEPFF 3101
>XP_005250084
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IRQRSELFF 9
            IRQRSE FF
Sbjct: 3093 IRQRSEPFF 3101
>XP_005250083
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IRQRSELFF 9
            IRQRSE FF
Sbjct: 3092 IRQRSEPFF 3100
>XP_005250082
          Length = 4983

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IRQRSELFF 9
            IRQRSE FF
Sbjct: 3093 IRQRSEPFF 3101
>NP_056020
          Length = 1134

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R+R ELFF
Sbjct: 465 REREELFF 472
>NP_115565
          Length = 1191

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R+R ELFF
Sbjct: 465 REREELFF 472
>XP_006720028
          Length = 1134

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R+R ELFF
Sbjct: 465 REREELFF 472
>XP_005268363
          Length = 1134

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R+R ELFF
Sbjct: 465 REREELFF 472
>XP_005268362
          Length = 1134

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R+R ELFF
Sbjct: 465 REREELFF 472
>XP_005268358
          Length = 1139

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R+R ELFF
Sbjct: 465 REREELFF 472
>XP_005268360
          Length = 1139

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R+R ELFF
Sbjct: 465 REREELFF 472
>XP_005268357
          Length = 1171

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R+R ELFF
Sbjct: 465 REREELFF 472
>XP_005268356
          Length = 1192

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R+R ELFF
Sbjct: 465 REREELFF 472
>NP_001035877
          Length = 953

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R RSELF+
Sbjct: 346 RNRSELFY 353
>NP_001035814
          Length = 953

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R RSELF+
Sbjct: 346 RNRSELFY 353
>NP_056062
          Length = 956

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R RSELF+
Sbjct: 349 RNRSELFY 356
>XP_006721215
          Length = 943

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R RSELF+
Sbjct: 346 RNRSELFY 353
>XP_006721214
          Length = 953

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R RSELF+
Sbjct: 346 RNRSELFY 353
>XP_006721213
          Length = 953

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R RSELF+
Sbjct: 346 RNRSELFY 353
>XP_006721212
          Length = 953

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R RSELF+
Sbjct: 346 RNRSELFY 353
>XP_005255869
          Length = 953

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R RSELF+
Sbjct: 346 RNRSELFY 353
>XP_006721211
          Length = 953

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQRSELFF 9
           R RSELF+
Sbjct: 346 RNRSELFY 353
>NP_579875
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RQRSELFF 9
          R+R+E FF
Sbjct: 56 RERNEYFF 63
>NP_758847
          Length = 425

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RQRSELFF 9
          R+R+E FF
Sbjct: 56 RERNEYFF 63
>NP_001349
          Length = 795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IRQRSEL 7
           +RQRS+L
Sbjct: 280 VRQRSDL 286
>NP_001253969
          Length = 1074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IRQRSELF 8
           +RQR +LF
Sbjct: 921 LRQRRQLF 928
>NP_004964
          Length = 1246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    IRQRSELF 8
            +RQR +LF
Sbjct: 1093 LRQRRQLF 1100
>NP_066554
          Length = 1020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RQRSEL 7
           RQRSEL
Sbjct: 344 RQRSEL 349
>NP_891554
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IRQRSELFF 9
           +RQ SEL +
Sbjct: 792 LRQESELMY 800
>NP_001243404
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RQRSEL 7
           RQRSEL
Sbjct: 360 RQRSEL 365
>XP_005261676
          Length = 986

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RQRSEL 7
           RQRSEL
Sbjct: 344 RQRSEL 349
>XP_006724320
          Length = 1006

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RQRSEL 7
           RQRSEL
Sbjct: 344 RQRSEL 349
>XP_005250695
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RQRSELF 8
           +QRS+LF
Sbjct: 161 KQRSDLF 167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RQRSELF 8
           +QRS+LF
Sbjct: 272 KQRSDLF 278
>XP_005249146
          Length = 1074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IRQRSELF 8
           +RQR +LF
Sbjct: 921 LRQRRQLF 928
>NP_001929
          Length = 176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IRQRSELF 8
           +RQ+ ELF
Sbjct: 119 LRQQQELF 126
>NP_115540
          Length = 561

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RQRSELF 8
           +QRS+LF
Sbjct: 368 KQRSDLF 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RQRSELF 8
           +QRS+LF
Sbjct: 479 KQRSDLF 485
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.348    0.155    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 399,099
Number of extensions: 573
Number of successful extensions: 43
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)

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