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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              19     665
NP_444254                                                              19     665
NP_444253                                                              19     665
NP_444255                                                              19     665
XP_005247551                                                           19     665
XP_006713712                                                           19     665
XP_005247549                                                           19     665
NP_001185791                                                           17    4312
NP_060722                                                              17    4312
XP_005273234                                                           17    4312
XP_006711494                                                           17    4312
NP_003310                                                              16    5631
NP_597676                                                              16    5631
NP_001243779                                                           16    5631
NP_596869                                                              16    5631
XP_005246888                                                           16    5631
XP_006712788                                                           16    5631
XP_006712787                                                           16    5631
XP_006712786                                                           16    5631
NP_001254479                                                           16    5631
NP_597681                                                              16    5631
NP_663622                                                              15    9605
NP_085058                                                              15    9605
NP_000709                                                              15    9605
NP_001230741                                                           15    9605
XP_005256204                                                           15    9605
XP_005256203                                                           15    9605
XP_005251162                                                           15    9605
XP_005251161                                                           15    9605
XP_005251159                                                           15    9605

>NP_444256
          Length = 1794

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IRQEISIVN 9
            IRQEISI+N
Sbjct: 1437 IRQEISIMN 1445



>NP_444254
          Length = 1845

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IRQEISIVN 9
            IRQEISI+N
Sbjct: 1437 IRQEISIMN 1445



>NP_444253
          Length = 1914

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IRQEISIVN 9
            IRQEISI+N
Sbjct: 1506 IRQEISIMN 1514



>NP_444255
          Length = 1863

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IRQEISIVN 9
            IRQEISI+N
Sbjct: 1506 IRQEISIMN 1514



>XP_005247551
          Length = 1855

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IRQEISIVN 9
            IRQEISI+N
Sbjct: 1447 IRQEISIMN 1455



>XP_006713712
          Length = 1923

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IRQEISIVN 9
            IRQEISI+N
Sbjct: 1516 IRQEISIMN 1524



>XP_005247549
          Length = 1924

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IRQEISIVN 9
            IRQEISI+N
Sbjct: 1516 IRQEISIMN 1524



>NP_001185791
          Length = 411

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQEISIVN 9
           RQE S+VN
Sbjct: 320 RQETSVVN 327



>NP_060722
          Length = 350

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQEISIVN 9
           RQE S+VN
Sbjct: 259 RQETSVVN 266



>XP_005273234
          Length = 273

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQEISIVN 9
           RQE S+VN
Sbjct: 182 RQETSVVN 189



>XP_006711494
          Length = 280

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQEISIVN 9
           RQE S+VN
Sbjct: 189 RQETSVVN 196



>NP_003310
          Length = 26926

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1     IRQEISIVN 9
             +++EISI+N
Sbjct: 24795 VKKEISILN 24803



>NP_597676
          Length = 27051

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1     IRQEISIVN 9
             +++EISI+N
Sbjct: 24920 VKKEISILN 24928



>NP_001243779
          Length = 34350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1     IRQEISIVN 9
             +++EISI+N
Sbjct: 32219 VKKEISILN 32227



>NP_596869
          Length = 33423

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1     IRQEISIVN 9
             +++EISI+N
Sbjct: 31292 VKKEISILN 31300



>XP_005246888
          Length = 22663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1     IRQEISIVN 9
             +++EISI+N
Sbjct: 20532 VKKEISILN 20540



>XP_006712788
          Length = 24022

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1     IRQEISIVN 9
             +++EISI+N
Sbjct: 21891 VKKEISILN 21899



>XP_006712787
          Length = 33978

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1     IRQEISIVN 9
             +++EISI+N
Sbjct: 31847 VKKEISILN 31855



>XP_006712786
          Length = 35484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1     IRQEISIVN 9
             +++EISI+N
Sbjct: 33353 VKKEISILN 33361



>NP_001254479
          Length = 35991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1     IRQEISIVN 9
             +++EISI+N
Sbjct: 33860 VKKEISILN 33868



>NP_597681
          Length = 27118

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1     IRQEISIVN 9
             +++EISI+N
Sbjct: 24987 VKKEISILN 24995



>NP_663622
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQEISIVN 9
           R+EI I+N
Sbjct: 929 RKEIEIIN 936



>NP_085058
          Length = 974

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IRQEISIVN 9
           ++QE S++N
Sbjct: 391 LQQEFSLIN 399



>NP_000709
          Length = 2339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    IRQEISIV 8
            +R+EIS+V
Sbjct: 1821 LRKEISVV 1828



>NP_001230741
          Length = 2237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    IRQEISIV 8
            +R+EIS+V
Sbjct: 1821 LRKEISVV 1828



>XP_005256204
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  IRQEISIVN 9
          ++QE S++N
Sbjct: 84 LQQEFSLIN 92



>XP_005256203
          Length = 993

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IRQEISIVN 9
           ++QE S++N
Sbjct: 391 LQQEFSLIN 399



>XP_005251162
          Length = 961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQEISIVN 9
           R+EI I+N
Sbjct: 824 RKEIEIIN 831



>XP_005251161
          Length = 987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQEISIVN 9
           R+EI I+N
Sbjct: 850 RKEIEIIN 857



>XP_005251159
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQEISIVN 9
           R+EI I+N
Sbjct: 898 RKEIEIIN 905



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.139    0.328 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 371,557
Number of extensions: 388
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)