Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     509
XP_005262424                                                           20     509
NP_997290                                                              17    2528
NP_817123                                                              17    4312
NP_073600                                                              16    5631
NP_001128567                                                           16    5631
NP_742056                                                              16    7355
NP_001275504                                                           16    7355
NP_001275503                                                           16    7355
NP_742055                                                              16    7355
NP_742057                                                              16    7355
NP_000494                                                              16    7355
NP_851999                                                              16    7355
NP_001185461                                                           16    7355
NP_001157861                                                           16    7355
NP_001157860                                                           16    7355
NP_001157859                                                           16    7355
NP_001157858                                                           16    7355
NP_060217                                                              16    7355
NP_057424                                                              16    7355
NP_065741                                                              16    7355
NP_001185462                                                           16    7355
XP_006716659                                                           16    7355
XP_005251053                                                           16    7355
XP_005251052                                                           16    7355
XP_006716658                                                           16    7355
XP_005251051                                                           16    7355
XP_005251050                                                           16    7355
XP_006716657                                                           16    7355
XP_006716500                                                           16    7355
XP_006716499                                                           16    7355
XP_005251241                                                           16    7355
XP_006716498                                                           16    7355
XP_005251239                                                           16    7355
NP_060472                                                              16    7355
XP_006720114                                                           15    9605
XP_006710110                                                           15    9605
NP_001001872                                                           15    9605
>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RMTSSNTQ 9
            RMTSSNTQ
Sbjct: 1869 RMTSSNTQ 1876
>XP_005262424
          Length = 2875

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RMTSSNTQ 9
            RMTSSNTQ
Sbjct: 1664 RMTSSNTQ 1671
>NP_997290
          Length = 438

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   IRMTSSN 7
           IRMTSSN
Sbjct: 188 IRMTSSN 194
>NP_817123
          Length = 1040

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   MTSSNTQ 9
           MT+SNTQ
Sbjct: 700 MTASNTQ 706
>NP_073600
          Length = 1204

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RMTSSNTQ 9
           R+T+SNT+
Sbjct: 547 RLTASNTE 554
>NP_001128567
          Length = 1204

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RMTSSNTQ 9
           R+T+SNT+
Sbjct: 547 RLTASNTE 554
>NP_742056
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSSNT 8
           MTSSNT
Sbjct: 235 MTSSNT 240
>NP_001275504
          Length = 470

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSSNT 8
           MTSSNT
Sbjct: 118 MTSSNT 123
>NP_001275503
          Length = 586

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSSNT 8
           MTSSNT
Sbjct: 234 MTSSNT 239
>NP_742055
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSSNT 8
           MTSSNT
Sbjct: 240 MTSSNT 245
>NP_742057
          Length = 559

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSSNT 8
           MTSSNT
Sbjct: 207 MTSSNT 212
>NP_000494
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSSNT 8
           MTSSNT
Sbjct: 240 MTSSNT 245
>NP_851999
          Length = 839

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT SNT+
Sbjct: 295 KMTGSNTE 302
>NP_001185461
          Length = 874

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT SNT+
Sbjct: 303 KMTGSNTE 310
>NP_001157861
          Length = 1391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    IRMTSSN 7
            +RMTS+N
Sbjct: 1051 VRMTSAN 1057
>NP_001157860
          Length = 1504

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    IRMTSSN 7
            +RMTS+N
Sbjct: 1051 VRMTSAN 1057
>NP_001157859
          Length = 1509

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    IRMTSSN 7
            +RMTS+N
Sbjct: 1056 VRMTSAN 1062
>NP_001157858
          Length = 1609

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    IRMTSSN 7
            +RMTS+N
Sbjct: 1051 VRMTSAN 1057
>NP_060217
          Length = 2542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    TSSNTQ 9
            TSSNTQ
Sbjct: 2073 TSSNTQ 2078
>NP_057424
          Length = 1601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    IRMTSSN 7
            +RMTS+N
Sbjct: 1051 VRMTSAN 1057
>NP_065741
          Length = 2617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    TSSNTQ 9
            TSSNTQ
Sbjct: 2073 TSSNTQ 2078
>NP_001185462
          Length = 873

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT SNT+
Sbjct: 302 KMTGSNTE 309
>XP_006716659
          Length = 653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT SNT+
Sbjct: 303 KMTGSNTE 310
>XP_005251053
          Length = 826

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT SNT+
Sbjct: 233 KMTGSNTE 240
>XP_005251052
          Length = 828

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT SNT+
Sbjct: 235 KMTGSNTE 242
>XP_006716658
          Length = 847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT SNT+
Sbjct: 303 KMTGSNTE 310
>XP_005251051
          Length = 888

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT SNT+
Sbjct: 295 KMTGSNTE 302
>XP_005251050
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT SNT+
Sbjct: 302 KMTGSNTE 309
>XP_006716657
          Length = 896

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT SNT+
Sbjct: 303 KMTGSNTE 310
>XP_006716500
          Length = 475

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSSNT 8
           MTSSNT
Sbjct: 123 MTSSNT 128
>XP_006716499
          Length = 559

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSSNT 8
           MTSSNT
Sbjct: 207 MTSSNT 212
>XP_005251241
          Length = 571

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSSNT 8
           MTSSNT
Sbjct: 219 MTSSNT 224
>XP_006716498
          Length = 591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSSNT 8
           MTSSNT
Sbjct: 239 MTSSNT 244
>XP_005251239
          Length = 621

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSSNT 8
           MTSSNT
Sbjct: 269 MTSSNT 274
>NP_060472
          Length = 846

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT SNT+
Sbjct: 302 KMTGSNTE 309
>XP_006720114
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT+ NTQ
Sbjct: 259 QMTADNTQ 266
>XP_006710110
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RMTSSNT 8
           ++TSSNT
Sbjct: 101 KLTSSNT 107
>NP_001001872
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RMTSSNTQ 9
           +MT+ NTQ
Sbjct: 259 QMTADNTQ 266
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.105    0.245 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 306,016
Number of extensions: 361
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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