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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004680                                                              17    3301
NP_055634                                                              16    5631
NP_001004334                                                           16    7355
NP_000978                                                              15    9605
NP_975010                                                              15    9605
NP_079416                                                              15    9605
NP_001712                                                              15    9605
XP_006724573                                                           15    9605
XP_005256807                                                           15    9605
XP_005256806                                                           15    9605
XP_005253682                                                           15    9605
XP_006719674                                                           15    9605
XP_005253681                                                           15    9605
XP_005265980                                                           15    9605
XP_005265979                                                           15    9605
XP_005265978                                                           15    9605
NP_057177                                                              15    9605

>NP_004680
          Length = 715

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   IRKLMSSS 8
           IRKL+SSS
Sbjct: 668 IRKLLSSS 675



>NP_055634
          Length = 708

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IRKLMSSS 8
           +RKL+SSS
Sbjct: 318 VRKLISSS 325



>NP_001004334
          Length = 2367

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RKLMSSSL 9
           R+L+SSSL
Sbjct: 752 RRLLSSSL 759



>NP_000978
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RKLMSSSL 9
          RK+MSS L
Sbjct: 27 RKIMSSPL 34



>NP_975010
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IRKLMSSS 8
           +RKL SSS
Sbjct: 246 VRKLKSSS 253



>NP_079416
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RKLMSSS 8
           +KLMSSS
Sbjct: 167 KKLMSSS 173



>NP_001712
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IRKLMSSS 8
           +RKL SSS
Sbjct: 246 VRKLKSSS 253



>XP_006724573
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IRKLMSSS 8
           +RKL SSS
Sbjct: 246 VRKLKSSS 253



>XP_005256807
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RKLMSSSL 9
          RK+MSS L
Sbjct: 27 RKIMSSPL 34



>XP_005256806
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RKLMSSSL 9
          RK+MSS L
Sbjct: 27 RKIMSSPL 34



>XP_005253682
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RKLMSSS 8
           +KLMSSS
Sbjct: 167 KKLMSSS 173



>XP_006719674
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RKLMSSS 8
           +KLMSSS
Sbjct: 167 KKLMSSS 173



>XP_005253681
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RKLMSSS 8
           +KLMSSS
Sbjct: 167 KKLMSSS 173



>XP_005265980
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RKLMSSSL 9
          RK+MSS L
Sbjct: 27 RKIMSSPL 34



>XP_005265979
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RKLMSSSL 9
          RK+MSS L
Sbjct: 27 RKIMSSPL 34



>XP_005265978
          Length = 174

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RKLMSSSL 9
          RK+MSS L
Sbjct: 56 RKIMSSPL 63



>NP_057177
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RKLMSSSL 9
          RK+MSS L
Sbjct: 27 RKIMSSPL 34



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.126    0.277 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 315,074
Number of extensions: 628
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)