Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064450                                                              18    1135
XP_005273257                                                           18    1135
NP_001193928                                                           17    4312
NP_001154976                                                           17    4312
NP_733844                                                              17    4312
NP_003739                                                              17    4312
NP_009105                                                              17    4312
XP_005253886                                                           17    4312
XP_006719269                                                           17    4312
XP_006726466                                                           16    5631
XP_006712969                                                           16    5631
XP_006710208                                                           16    5631
NP_004689                                                              16    7355
NP_663622                                                              16    7355
XP_005251162                                                           16    7355
XP_005251161                                                           16    7355
XP_005251160                                                           16    7355
XP_005251159                                                           16    7355
NP_001138560                                                           16    7355
NP_004278                                                              15    9605
NP_001012529                                                           15    9605
NP_000032                                                              15    9605
NP_473363                                                              15    9605
NP_003240                                                              15    9605
XP_005258924                                                           15    9605
XP_005257902                                                           15    9605
XP_005257901                                                           15    9605
XP_006722255                                                           15    9605
XP_006722254                                                           15    9605
XP_006722253                                                           15    9605
XP_006722252                                                           15    9605
XP_006722251                                                           15    9605
XP_005257900                                                           15    9605
>NP_064450
          Length = 1722

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IQAKIDQAA 9
            IQAKIDQ A
Sbjct: 1618 IQAKIDQEA 1626
>XP_005273257
          Length = 1726

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IQAKIDQAA 9
            IQAKIDQ A
Sbjct: 1622 IQAKIDQEA 1630
>NP_001193928
          Length = 2069

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    IQAKIDQAA 9
            +QAK+DQ A
Sbjct: 1268 LQAKMDQPA 1276
>NP_001154976
          Length = 503

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IQAKIDQAA 9
           IQA+ID AA
Sbjct: 96  IQAEIDAAA 104
>NP_733844
          Length = 563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IQAKIDQAA 9
           IQA+ID AA
Sbjct: 156 IQAEIDAAA 164
>NP_003739
          Length = 563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IQAKIDQAA 9
           IQA+ID AA
Sbjct: 156 IQAEIDAAA 164
>NP_009105
          Length = 2027

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    IQAKIDQAA 9
            +QAK+DQ A
Sbjct: 1226 LQAKMDQPA 1234
>XP_005253886
          Length = 1545

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IQAKIDQAA 9
           +QAK+DQ A
Sbjct: 760 LQAKMDQPA 768
>XP_006719269
          Length = 2054

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    IQAKIDQAA 9
            +QAK+DQ A
Sbjct: 1268 LQAKMDQPA 1276
>XP_006726466
          Length = 1310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   IQAKIDQA 8
           +QA++DQA
Sbjct: 868 LQARLDQA 875
>XP_006712969
          Length = 1310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   IQAKIDQA 8
           +QA++DQA
Sbjct: 868 LQARLDQA 875
>XP_006710208
          Length = 666

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   IQAKIDQA 8
           +QA++DQA
Sbjct: 224 LQARLDQA 231
>NP_004689
          Length = 683

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IQAKIDQAA 9
           +QA+I QAA
Sbjct: 351 LQAEISQAA 359
>NP_663622
          Length = 1066

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IQAKIDQ 7
           +QAK+DQ
Sbjct: 712 MQAKVDQ 718
>XP_005251162
          Length = 961

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IQAKIDQ 7
           +QAK+DQ
Sbjct: 607 MQAKVDQ 613
>XP_005251161
          Length = 987

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IQAKIDQ 7
           +QAK+DQ
Sbjct: 633 MQAKVDQ 639
>XP_005251160
          Length = 1001

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IQAKIDQ 7
           +QAK+DQ
Sbjct: 712 MQAKVDQ 718
>XP_005251159
          Length = 1035

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IQAKIDQ 7
           +QAK+DQ
Sbjct: 712 MQAKVDQ 718
>NP_001138560
          Length = 970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IQAKIDQ 7
           +QAK+DQ
Sbjct: 712 MQAKVDQ 718
>NP_004278
          Length = 212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IQAKIDQ 7
          IQA IDQ
Sbjct: 37 IQASIDQ 43
>NP_001012529
          Length = 195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IQAKIDQ 7
          IQA IDQ
Sbjct: 37 IQASIDQ 43
>NP_000032
          Length = 317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  QAKIDQA 8
          QAK++QA
Sbjct: 17 QAKVEQA 23
>NP_473363
          Length = 213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IQAKIDQ 7
          IQA IDQ
Sbjct: 37 IQASIDQ 43
>NP_003240
          Length = 689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IQAKIDQA 8
           + AK+DQA
Sbjct: 556 VLAKVDQA 563
>XP_005258924
          Length = 343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  QAKIDQA 8
          QAK++QA
Sbjct: 43 QAKVEQA 49
>XP_005257902
          Length = 147

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IQAKIDQ 7
          IQA IDQ
Sbjct: 19 IQASIDQ 25
>XP_005257901
          Length = 165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IQAKIDQ 7
          IQA IDQ
Sbjct: 37 IQASIDQ 43
>XP_006722255
          Length = 179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IQAKIDQ 7
          IQA IDQ
Sbjct: 37 IQASIDQ 43
>XP_006722254
          Length = 202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IQAKIDQ 7
          IQA IDQ
Sbjct: 37 IQASIDQ 43
>XP_006722253
          Length = 214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IQAKIDQ 7
          IQA IDQ
Sbjct: 37 IQASIDQ 43
>XP_006722252
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IQAKIDQ 7
          IQA IDQ
Sbjct: 37 IQASIDQ 43
>XP_006722251
          Length = 239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IQAKIDQ 7
          IQA IDQ
Sbjct: 19 IQASIDQ 25
>XP_005257900
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IQAKIDQ 7
          IQA IDQ
Sbjct: 37 IQASIDQ 43
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.122    0.287 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 299,387
Number of extensions: 470
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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