Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              21     175
XP_005262424                                                           21     175
NP_005036                                                              16    7355
NP_774959                                                              16    7355
NP_001034180                                                           16    7355
NP_002756                                                              16    7355
NP_006080                                                              16    7355
NP_001128119                                                           16    7355
NP_940997                                                              16    7355
XP_006724521                                                           16    7355
XP_006724520                                                           16    7355
XP_006713972                                                           16    7355
XP_006713971                                                           16    7355
NP_000522                                                              15    9605
NP_000258                                                              15    9605
NP_001035957                                                           15    9605
NP_001026881                                                           15    9605
XP_006721990                                                           15    9605
XP_006721989                                                           15    9605
XP_005258041                                                           15    9605
XP_006721988                                                           15    9605
XP_006721987                                                           15    9605
XP_006721986                                                           15    9605
XP_005258040                                                           15    9605
XP_006721985                                                           15    9605
XP_006714957                                                           15    9605
XP_006714956                                                           15    9605
XP_006714955                                                           15    9605
>NP_722576
          Length = 3080

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IPVNNAAFT 9
            IPVNN+AFT
Sbjct: 1565 IPVNNSAFT 1573

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    IPVNNAAFT 9
            +PVN  AFT
Sbjct: 1516 LPVNVTAFT 1524
>XP_005262424
          Length = 2875

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IPVNNAAFT 9
            IPVNN+AFT
Sbjct: 1360 IPVNNSAFT 1368

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    IPVNNAAFT 9
            +PVN  AFT
Sbjct: 1311 LPVNVTAFT 1319
>NP_005036
          Length = 3460

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IPVNNAAFT 9
           IP+ NAA T
Sbjct: 637 IPLPNAALT 645
>NP_774959
          Length = 3458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IPVNNAAFT 9
           IP+ NAA T
Sbjct: 637 IPLPNAALT 645
>NP_001034180
          Length = 321

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VNNAAF 8
           +NNAAF
Sbjct: 264 INNAAF 269
>NP_002756
          Length = 318

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VNNAAF 8
           +NNAAF
Sbjct: 261 INNAAF 266
>NP_006080
          Length = 700

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PVNNAAF 8
           PVN+A+F
Sbjct: 438 PVNSASF 444
>NP_001128119
          Length = 814

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IPVNNAA 7
           +PVN+AA
Sbjct: 777 VPVNSAA 783
>NP_940997
          Length = 730

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IPVNNAA 7
           +PVN+AA
Sbjct: 693 VPVNSAA 699
>XP_006724521
          Length = 609

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PVNNAAF 8
           PVN+A+F
Sbjct: 438 PVNSASF 444
>XP_006724520
          Length = 617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PVNNAAF 8
           PVN+A+F
Sbjct: 438 PVNSASF 444
>XP_006713972
          Length = 751

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IPVNNAA 7
           +PVN+AA
Sbjct: 714 VPVNSAA 720
>XP_006713971
          Length = 835

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IPVNNAA 7
           +PVN+AA
Sbjct: 798 VPVNSAA 804
>NP_000522
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  IPVNNAAF 8
          I +NNAAF
Sbjct: 7  ILLNNAAF 14
>NP_000258
          Length = 2818

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    IPVNNAAF 8
            IP NN  F
Sbjct: 1879 IPANNTLF 1886
>NP_001035957
          Length = 2839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    IPVNNAAF 8
            IP NN  F
Sbjct: 1900 IPANNTLF 1907
>NP_001026881
          Length = 290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IPVNNAA 7
           IP+NN A
Sbjct: 107 IPLNNGA 113
>XP_006721990
          Length = 2823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    IPVNNAAF 8
            IP NN  F
Sbjct: 1910 IPANNTLF 1917
>XP_006721989
          Length = 2823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    IPVNNAAF 8
            IP NN  F
Sbjct: 1910 IPANNTLF 1917
>XP_005258041
          Length = 2836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    IPVNNAAF 8
            IP NN  F
Sbjct: 1879 IPANNTLF 1886
>XP_006721988
          Length = 2846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    IPVNNAAF 8
            IP NN  F
Sbjct: 1889 IPANNTLF 1896
>XP_006721987
          Length = 2849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    IPVNNAAF 8
            IP NN  F
Sbjct: 1910 IPANNTLF 1917
>XP_006721986
          Length = 2854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    IPVNNAAF 8
            IP NN  F
Sbjct: 1897 IPANNTLF 1904
>XP_005258040
          Length = 2857

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    IPVNNAAF 8
            IP NN  F
Sbjct: 1900 IPANNTLF 1907
>XP_006721985
          Length = 2867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    IPVNNAAF 8
            IP NN  F
Sbjct: 1910 IPANNTLF 1917
>XP_006714957
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  IPVNNAA 7
          IP+NN A
Sbjct: 62 IPLNNGA 68
>XP_006714956
          Length = 187

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IPVNNAA 7
           IP+NN A
Sbjct: 107 IPLNNGA 113
>XP_006714955
          Length = 198

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  IPVNNAA 7
          IP+NN A
Sbjct: 15 IPLNNGA 21
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.130    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 526,525
Number of extensions: 939
Number of successful extensions: 30
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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