Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              20     390
NP_001243495                                                           17    3301
NP_004092                                                              17    3301
NP_065434                                                              17    3301
XP_005254491                                                           17    3301
XP_005268848                                                           17    3301
XP_005268846                                                           17    3301
XP_005268845                                                           17    3301
XP_005268844                                                           17    3301
XP_005268843                                                           17    3301
NP_001268669                                                           17    4312
NP_079411                                                              17    4312
NP_001268668                                                           17    4312
NP_066301                                                              17    4312
NP_009171                                                              17    4312
XP_006722614                                                           17    4312
XP_005258412                                                           17    4312
XP_005258411                                                           17    4312
XP_005258409                                                           17    4312
XP_005258408                                                           17    4312
XP_006722613                                                           17    4312
XP_005258407                                                           17    4312
XP_005258406                                                           17    4312
XP_005258405                                                           17    4312
XP_005258404                                                           17    4312
XP_005258403                                                           17    4312
XP_005251089                                                           17    4312
XP_005251088                                                           17    4312
XP_005244919                                                           17    4312
NP_001135780                                                           17    4312
NP_060720                                                              16    5631
NP_001166033                                                           16    5631
NP_079461                                                              16    5631
XP_005257260                                                           16    5631
XP_005257258                                                           16    5631
XP_006721872                                                           16    5631
XP_006721871                                                           16    5631
XP_006721870                                                           16    5631
XP_006721869                                                           16    5631
XP_006716420                                                           16    5631
XP_006716419                                                           16    5631
NP_001138631                                                           16    5631
NP_001018048                                                           16    7355
NP_056001                                                              16    7355
NP_001018047                                                           16    7355
NP_150648                                                              16    7355
NP_055855                                                              16    7355
NP_689884                                                              16    7355
NP_001171471                                                           16    7355
NP_002022                                                              15    9605
NP_001263280                                                           15    9605
NP_054704                                                              15    9605
NP_054702                                                              15    9605
XP_005266938                                                           15    9605
XP_005266939                                                           15    9605
XP_005266937                                                           15    9605
>NP_073713
          Length = 985

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IPFKMAYET 9
           IP KMAYET
Sbjct: 264 IPSKMAYET 272
>NP_001243495
          Length = 352

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IPFKMAY 7
           +PFK+AY
Sbjct: 124 LPFKIAY 130
>NP_004092
          Length = 374

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IPFKMAY 7
           +PFK+AY
Sbjct: 146 LPFKIAY 152
>NP_065434
          Length = 573

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PFKMAYE 8
           PF++AYE
Sbjct: 435 PFQLAYE 441
>XP_005254491
          Length = 151

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IPFKMAY 7
           IPF+M+Y
Sbjct: 143 IPFRMSY 149
>XP_005268848
          Length = 422

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PFKMAYE 8
           PF++AYE
Sbjct: 284 PFQLAYE 290
>XP_005268846
          Length = 436

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PFKMAYE 8
           PF++AYE
Sbjct: 298 PFQLAYE 304
>XP_005268845
          Length = 462

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PFKMAYE 8
           PF++AYE
Sbjct: 324 PFQLAYE 330
>XP_005268844
          Length = 483

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PFKMAYE 8
           PF++AYE
Sbjct: 345 PFQLAYE 351
>XP_005268843
          Length = 547

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PFKMAYE 8
           PF++AYE
Sbjct: 409 PFQLAYE 415
>NP_001268669
          Length = 1622

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1244 FKMDYET 1250
>NP_079411
          Length = 1439

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1069 FKMDYET 1075
>NP_001268668
          Length = 1422

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1052 FKMDYET 1058
>NP_066301
          Length = 538

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   IPFKMAYET 9
           IP KM Y T
Sbjct: 244 IPLKMCYVT 252
>NP_009171
          Length = 340

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IPFKMAYE 8
           +PF+ AYE
Sbjct: 136 LPFEKAYE 143
>XP_006722614
          Length = 1589

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1219 FKMDYET 1225
>XP_005258412
          Length = 1401

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1031 FKMDYET 1037
>XP_005258411
          Length = 1409

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1031 FKMDYET 1037
>XP_005258409
          Length = 1430

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1052 FKMDYET 1058
>XP_005258408
          Length = 1447

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1069 FKMDYET 1075
>XP_006722613
          Length = 1523

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1145 FKMDYET 1151
>XP_005258407
          Length = 1533

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1244 FKMDYET 1250
>XP_005258406
          Length = 1559

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1181 FKMDYET 1187
>XP_005258405
          Length = 1597

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1219 FKMDYET 1225
>XP_005258404
          Length = 1605

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1227 FKMDYET 1233
>XP_005258403
          Length = 1614

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FKMAYET 9
            FKM YET
Sbjct: 1244 FKMDYET 1250
>XP_005251089
          Length = 480

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   IPFKMAYET 9
           IP KM Y T
Sbjct: 342 IPLKMCYVT 350
>XP_005251088
          Length = 636

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   IPFKMAYET 9
           IP KM Y T
Sbjct: 342 IPLKMCYVT 350
>XP_005244919
          Length = 210

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  IPFKMAYE 8
          +PF+ AYE
Sbjct: 6  LPFEKAYE 13
>NP_001135780
          Length = 2156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    PFKMAYE 8
            PFK+A+E
Sbjct: 1862 PFKVAFE 1868
>NP_060720
          Length = 658

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   IPFKMAYE 8
           +PFK  YE
Sbjct: 507 LPFKRRYE 514
>NP_001166033
          Length = 634

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   IPFKMAYE 8
           +PFK  YE
Sbjct: 483 LPFKRRYE 490
>NP_079461
          Length = 1990

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    IPFKMAYE 8
            IP K AYE
Sbjct: 1810 IPLKPAYE 1817
>XP_005257260
          Length = 1990

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    IPFKMAYE 8
            IP K AYE
Sbjct: 1810 IPLKPAYE 1817
>XP_005257258
          Length = 2000

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    IPFKMAYE 8
            IP K AYE
Sbjct: 1820 IPLKPAYE 1827
>XP_006721872
          Length = 2037

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    IPFKMAYE 8
            IP K AYE
Sbjct: 1857 IPLKPAYE 1864
>XP_006721871
          Length = 2037

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    IPFKMAYE 8
            IP K AYE
Sbjct: 1857 IPLKPAYE 1864
>XP_006721870
          Length = 2064

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    IPFKMAYE 8
            IP K AYE
Sbjct: 1884 IPLKPAYE 1891
>XP_006721869
          Length = 2074

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    IPFKMAYE 8
            IP K AYE
Sbjct: 1894 IPLKPAYE 1901
>XP_006716420
          Length = 646

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   IPFKMAYE 8
           +PFK  YE
Sbjct: 507 LPFKRRYE 514
>XP_006716419
          Length = 676

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   IPFKMAYE 8
           +PFK  YE
Sbjct: 507 LPFKRRYE 514
>NP_001138631
          Length = 637

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   IPFKMAYE 8
           +PFK  YE
Sbjct: 486 LPFKRRYE 493
>NP_001018048
          Length = 3069

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    IPFKMAY 7
            +P+K+AY
Sbjct: 2117 LPYKIAY 2123
>NP_056001
          Length = 3095

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    IPFKMAY 7
            +P+K+AY
Sbjct: 2117 LPYKIAY 2123
>NP_001018047
          Length = 3135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    IPFKMAY 7
            +P+K+AY
Sbjct: 2078 LPYKIAY 2084
>NP_150648
          Length = 3174

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    IPFKMAY 7
            +P+K+AY
Sbjct: 2117 LPYKIAY 2123
>NP_055855
          Length = 2098

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  PFKMAYET 9
          PFK AY +
Sbjct: 41 PFKSAYSS 48
>NP_689884
          Length = 451

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  PFKMAYET 9
          PFK AY +
Sbjct: 41 PFKSAYSS 48
>NP_001171471
          Length = 548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  PFKMAYET 9
          PFK AY +
Sbjct: 41 PFKSAYSS 48
>NP_002022
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   PFKMAYET 9
           PF ++Y+T
Sbjct: 216 PFDLSYKT 223
>NP_001263280
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   PFKMAYE 8
           PFK  YE
Sbjct: 373 PFKYIYE 379
>NP_054704
          Length = 96

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  IPFKMAYE 8
          I  KMAYE
Sbjct: 78 ISHKMAYE 85
>NP_054702
          Length = 111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   IPFKMAYE 8
           I  KMAYE
Sbjct: 93  ISHKMAYE 100
>XP_005266938
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   PFKMAYET 9
           PF ++Y+T
Sbjct: 216 PFDLSYKT 223
>XP_005266939
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   PFKMAYET 9
           PF ++Y+T
Sbjct: 216 PFDLSYKT 223
>XP_005266937
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   PFKMAYET 9
           PF ++Y+T
Sbjct: 216 PFDLSYKT 223
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.138    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 518,171
Number of extensions: 769
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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