Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              18    1482
NP_001087241                                                           18    1482
XP_005265799                                                           18    1482
XP_005265798                                                           18    1482
XP_005265797                                                           18    1482
NP_653310                                                              17    2528
XP_006712015                                                           17    2528
NP_996816                                                              17    4312
NP_001091681                                                           16    5631
NP_001091683                                                           16    5631
NP_001091682                                                           16    5631
NP_000852                                                              16    5631
NP_068370                                                              16    7355
NP_005494                                                              16    7355
NP_001123886                                                           16    7355
NP_006071                                                              16    7355
NP_001154                                                              16    7355
NP_001265043                                                           16    7355
NP_001265042                                                           16    7355
NP_001455                                                              16    7355
XP_006725606                                                           16    7355
XP_005254388                                                           16    7355
XP_006725182                                                           16    7355
XP_006720544                                                           16    7355
XP_006720543                                                           16    7355
XP_005252025                                                           16    7355
XP_005252024                                                           16    7355
XP_006717156                                                           16    7355
XP_005273525                                                           16    7355
XP_006715902                                                           16    7355
XP_006715901                                                           16    7355
XP_005250168                                                           16    7355
XP_005250167                                                           16    7355
XP_006710441                                                           16    7355
XP_005270554                                                           16    7355
NP_002026                                                              15    9605
NP_001631                                                              15    9605
XP_006723172                                                           15    9605
XP_006723171                                                           15    9605
XP_006723167                                                           15    9605
XP_006723166                                                           15    9605
XP_006723165                                                           15    9605
XP_006723164                                                           15    9605
XP_006723163                                                           15    9605
XP_006723162                                                           15    9605
XP_006723161                                                           15    9605
XP_005266734                                                           15    9605
XP_006722496                                                           15    9605
XP_005265155                                                           15    9605
XP_005265154                                                           15    9605
NP_060689                                                              15    9605
>NP_000213
          Length = 976

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   INYVGSTAS 9
           IN VGSTAS
Sbjct: 957 INSVGSTAS 965
>NP_001087241
          Length = 972

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   INYVGSTAS 9
           IN VGSTAS
Sbjct: 953 INSVGSTAS 961
>XP_005265799
          Length = 973

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   INYVGSTAS 9
           IN VGSTAS
Sbjct: 954 INSVGSTAS 962
>XP_005265798
          Length = 976

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   INYVGSTAS 9
           IN VGSTAS
Sbjct: 957 INSVGSTAS 965
>XP_005265797
          Length = 977

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   INYVGSTAS 9
           IN VGSTAS
Sbjct: 958 INSVGSTAS 966
>NP_653310
          Length = 529

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   INYVGSTAS 9
           +++VGSTAS
Sbjct: 93  VSHVGSTAS 101
>XP_006712015
          Length = 529

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   INYVGSTAS 9
           +++VGSTAS
Sbjct: 93  VSHVGSTAS 101
>NP_996816
          Length = 5202

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    NYVGSTAS 9
            N VGSTAS
Sbjct: 4913 NEVGSTAS 4920
>NP_001091681
          Length = 487

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   INYVGSTAS 9
           ++YV STAS
Sbjct: 106 MDYVASTAS 114
>NP_001091683
          Length = 487

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   INYVGSTAS 9
           ++YV STAS
Sbjct: 106 MDYVASTAS 114
>NP_001091682
          Length = 487

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   INYVGSTAS 9
           ++YV STAS
Sbjct: 106 MDYVASTAS 114
>NP_000852
          Length = 487

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   INYVGSTAS 9
           ++YV STAS
Sbjct: 106 MDYVASTAS 114
>NP_068370
          Length = 614

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  INYVGSTAS 9
          I Y+GS+ S
Sbjct: 14 ITYIGSSGS 22
>NP_005494
          Length = 749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NY+GST
Sbjct: 375 NYLGST 380
>NP_001123886
          Length = 737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NY+GST
Sbjct: 375 NYLGST 380
>NP_006071
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YVGSTA 8
           Y+GSTA
Sbjct: 502 YIGSTA 507
>NP_001154
          Length = 837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NY+GST
Sbjct: 464 NYLGST 469
>NP_001265043
          Length = 672

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NYVG+T
Sbjct: 276 NYVGAT 281
>NP_001265042
          Length = 716

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NYVG+T
Sbjct: 257 NYVGAT 262
>NP_001455
          Length = 735

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NYVG+T
Sbjct: 276 NYVGAT 281
>XP_006725606
          Length = 737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NY+GST
Sbjct: 375 NYLGST 380
>XP_005254388
          Length = 749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NY+GST
Sbjct: 375 NYLGST 380
>XP_006725182
          Length = 705

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NYVG+T
Sbjct: 276 NYVGAT 281
>XP_006720544
          Length = 778

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NY+GST
Sbjct: 375 NYLGST 380
>XP_006720543
          Length = 790

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NY+GST
Sbjct: 375 NYLGST 380
>XP_005252025
          Length = 826

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NY+GST
Sbjct: 464 NYLGST 469
>XP_005252024
          Length = 837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NY+GST
Sbjct: 464 NYLGST 469
>XP_006717156
          Length = 837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NY+GST
Sbjct: 464 NYLGST 469
>XP_005273525
          Length = 705

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           NYVG+T
Sbjct: 276 NYVGAT 281
>XP_006715902
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YVGSTA 8
           Y+GSTA
Sbjct: 502 YIGSTA 507
>XP_006715901
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YVGSTA 8
           Y+GSTA
Sbjct: 502 YIGSTA 507
>XP_005250168
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YVGSTA 8
           Y+GSTA
Sbjct: 502 YIGSTA 507
>XP_005250167
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YVGSTA 8
           Y+GSTA
Sbjct: 502 YIGSTA 507
>XP_006710441
          Length = 627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  INYVGSTAS 9
          +N+ GST+S
Sbjct: 64 LNFPGSTSS 72
>XP_005270554
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  INYVGSTAS 9
          +N+ GST+S
Sbjct: 64 LNFPGSTSS 72
>NP_002026
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   INYVGS 6
           INY+GS
Sbjct: 144 INYLGS 149
>NP_001631
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   INYVGST 7
           +NY GST
Sbjct: 535 VNYRGST 541
>XP_006723172
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  INYVGST 7
          +NY+G T
Sbjct: 17 VNYLGGT 23
>XP_006723171
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  INYVGST 7
          +NY+G T
Sbjct: 41 VNYLGGT 47
>XP_006723167
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  INYVGST 7
          +NY+G T
Sbjct: 17 VNYLGGT 23
>XP_006723166
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  INYVGST 7
          +NY+G T
Sbjct: 17 VNYLGGT 23
>XP_006723165
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  INYVGST 7
          +NY+G T
Sbjct: 17 VNYLGGT 23
>XP_006723164
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  INYVGST 7
          +NY+G T
Sbjct: 17 VNYLGGT 23
>XP_006723163
          Length = 455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  INYVGST 7
          +NY+G T
Sbjct: 41 VNYLGGT 47
>XP_006723162
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  INYVGST 7
          +NY+G T
Sbjct: 41 VNYLGGT 47
>XP_006723161
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  INYVGST 7
          +NY+G T
Sbjct: 41 VNYLGGT 47
>XP_005266734
          Length = 298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   INYVGS 6
           INY+GS
Sbjct: 144 INYLGS 149
>XP_006722496
          Length = 303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   INYVGS 6
           INY+GS
Sbjct: 115 INYLGS 120
>XP_005265155
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   INYVGST 7
           +NY GST
Sbjct: 434 VNYRGST 440
>XP_005265154
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   INYVGST 7
           +NY GST
Sbjct: 489 VNYRGST 495
>NP_060689
          Length = 849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYVGST 7
           N+VGST
Sbjct: 485 NFVGST 490
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.118    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 489,096
Number of extensions: 460
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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