Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001261                                                              20     299
XP_005271924                                                           20     299
XP_005271923                                                           20     299
XP_005254203                                                           16    5631
NP_055488                                                              16    5631
NP_055244                                                              16    7355
NP_001364                                                              15    9605
NP_003897                                                              15    9605
NP_787074                                                              15    9605
NP_055528                                                              15    9605
NP_005732                                                              15    9605
XP_006724128                                                           15    9605
XP_006724127                                                           15    9605
XP_005261262                                                           15    9605
XP_005261261                                                           15    9605
XP_005261263                                                           15    9605
XP_005261260                                                           15    9605
XP_006720898                                                           15    9605
XP_006720897                                                           15    9605
XP_006720896                                                           15    9605
XP_006720895                                                           15    9605
XP_006720894                                                           15    9605
XP_005255088                                                           15    9605
XP_006711798                                                           15    9605
NP_001968                                                              15    9605
>NP_001261
          Length = 1710

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   INNRNMIRT 9
           IN+RNMIRT
Sbjct: 565 INSRNMIRT 573
>XP_005271924
          Length = 1710

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   INNRNMIRT 9
           IN+RNMIRT
Sbjct: 565 INSRNMIRT 573
>XP_005271923
          Length = 1798

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   INNRNMIRT 9
           IN+RNMIRT
Sbjct: 565 INSRNMIRT 573
>XP_005254203
          Length = 848

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   INNRNMI 7
           INNRN +
Sbjct: 840 INNRNQV 846
>NP_055488
          Length = 297

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   INNRNM 6
           +NNRNM
Sbjct: 221 LNNRNM 226
>NP_055244
          Length = 984

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   INNRNM 6
           INNRN+
Sbjct: 443 INNRNL 448
>NP_001364
          Length = 4515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NRNMIRT 9
           N N+IRT
Sbjct: 448 NENLIRT 454
>NP_003897
          Length = 1980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   INNRNMIR 8
           INN NM +
Sbjct: 774 INNENMTK 781
>NP_787074
          Length = 459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NNRNMIRT 9
           NN NM+ T
Sbjct: 296 NNSNMVGT 303
>NP_055528
          Length = 233

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   INNRNM 6
           INNRN+
Sbjct: 190 INNRNV 195
>NP_005732
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NNRNMIR 8
           NN N+IR
Sbjct: 389 NNSNLIR 395
>XP_006724128
          Length = 1483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   INNRNMIR 8
           INN NM +
Sbjct: 277 INNENMTK 284
>XP_006724127
          Length = 1980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   INNRNMIR 8
           INN NM +
Sbjct: 774 INNENMTK 781
>XP_005261262
          Length = 1980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   INNRNMIR 8
           INN NM +
Sbjct: 774 INNENMTK 781
>XP_005261261
          Length = 1980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   INNRNMIR 8
           INN NM +
Sbjct: 774 INNENMTK 781
>XP_005261263
          Length = 1980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   INNRNMIR 8
           INN NM +
Sbjct: 774 INNENMTK 781
>XP_005261260
          Length = 1980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   INNRNMIR 8
           INN NM +
Sbjct: 774 INNENMTK 781
>XP_006720898
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NNRNMIR 8
           NN N+IR
Sbjct: 185 NNSNLIR 191
>XP_006720897
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NNRNMIR 8
           NN N+IR
Sbjct: 185 NNSNLIR 191
>XP_006720896
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NNRNMIR 8
           NN N+IR
Sbjct: 304 NNSNLIR 310
>XP_006720895
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NNRNMIR 8
           NN N+IR
Sbjct: 350 NNSNLIR 356
>XP_006720894
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NNRNMIR 8
           NN N+IR
Sbjct: 376 NNSNLIR 382
>XP_005255088
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NNRNMIR 8
           NN N+IR
Sbjct: 389 NNSNLIR 395
>XP_006711798
          Length = 4586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NRNMIRT 9
           N N+IRT
Sbjct: 448 NENLIRT 454
>NP_001968
          Length = 957

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   INNRNMIR 8
           +NNRN+ R
Sbjct: 884 LNNRNLGR 891
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.134    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 451,274
Number of extensions: 554
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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