Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              20     390
NP_078989                                                              17    4312
XP_005254725                                                           17    4312
XP_006720747                                                           17    4312
XP_005254724                                                           17    4312
XP_005254723                                                           17    4312
NP_001012779                                                           16    5631
XP_005272023                                                           16    5631
NP_005155                                                              16    5631
NP_001167558                                                           16    7355
NP_858045                                                              16    7355
NP_006525                                                              16    7355
NP_001167559                                                           16    7355
NP_056128                                                              16    7355
NP_001185594                                                           16    7355
XP_005271537                                                           16    7355
XP_005271535                                                           16    7355
XP_006718868                                                           16    7355
NP_001129386                                                           16    7355
NP_003310                                                              15    9605
NP_055509                                                              15    9605
NP_597676                                                              15    9605
NP_001138792                                                           15    9605
NP_001243779                                                           15    9605
NP_596869                                                              15    9605
NP_001096078                                                           15    9605
NP_004524                                                              15    9605
XP_005272296                                                           15    9605
XP_006717400                                                           15    9605
XP_006717399                                                           15    9605
XP_005272294                                                           15    9605
XP_005247178                                                           15    9605
XP_005246888                                                           15    9605
XP_006712788                                                           15    9605
XP_006712787                                                           15    9605
XP_006712786                                                           15    9605
NP_001254479                                                           15    9605
NP_597681                                                              15    9605

>NP_115803
          Length = 1156

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   INDGQSQIM 9
           IN+GQSQIM
Sbjct: 221 INNGQSQIM 229



>NP_078989
          Length = 304

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   INDGQSQIM 9
           INDG  QI+
Sbjct: 260 INDGNIQIL 268



>XP_005254725
          Length = 298

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   INDGQSQIM 9
           INDG  QI+
Sbjct: 260 INDGNIQIL 268



>XP_006720747
          Length = 306

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   INDGQSQIM 9
           INDG  QI+
Sbjct: 228 INDGNIQIL 236



>XP_005254724
          Length = 338

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   INDGQSQIM 9
           INDG  QI+
Sbjct: 260 INDGNIQIL 268



>XP_005254723
          Length = 338

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   INDGQSQIM 9
           INDG  QI+
Sbjct: 260 INDGNIQIL 268



>NP_001012779
          Length = 478

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   INDGQSQI 8
           I+DGQ Q+
Sbjct: 363 IDDGQGQV 370



>XP_005272023
          Length = 477

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   INDGQSQI 8
           I+DGQ Q+
Sbjct: 362 IDDGQGQV 369



>NP_005155
          Length = 740

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   DGQSQIM 9
           DGQ Q+M
Sbjct: 375 DGQKQVM 381



>NP_001167558
          Length = 1423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   INDGQS 6
           +NDGQS
Sbjct: 307 LNDGQS 312



>NP_858045
          Length = 1424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   INDGQS 6
           +NDGQS
Sbjct: 307 LNDGQS 312



>NP_006525
          Length = 1420

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   INDGQS 6
           +NDGQS
Sbjct: 307 LNDGQS 312



>NP_001167559
          Length = 1415

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   INDGQS 6
           +NDGQS
Sbjct: 307 LNDGQS 312



>NP_056128
          Length = 1544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    DGQSQIM 9
            D QSQIM
Sbjct: 1498 DSQSQIM 1504



>NP_001185594
          Length = 1525

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    DGQSQIM 9
            D QSQIM
Sbjct: 1479 DSQSQIM 1485



>XP_005271537
          Length = 1441

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    DGQSQIM 9
            D QSQIM
Sbjct: 1395 DSQSQIM 1401



>XP_005271535
          Length = 1504

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    DGQSQIM 9
            D QSQIM
Sbjct: 1458 DSQSQIM 1464



>XP_006718868
          Length = 1519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    DGQSQIM 9
            D QSQIM
Sbjct: 1473 DSQSQIM 1479



>NP_001129386
          Length = 1503

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    DGQSQI 8
            DGQSQ+
Sbjct: 1261 DGQSQV 1266



>NP_003310
          Length = 26926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2     NDGQSQI 8
             NDG SQ+
Sbjct: 12227 NDGGSQV 12233



>NP_055509
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   DGQSQIM 9
           DGQ Q+M
Sbjct: 156 DGQRQLM 162



>NP_597676
          Length = 27051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2     NDGQSQI 8
             NDG SQ+
Sbjct: 12352 NDGGSQV 12358



>NP_001138792
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   DGQSQIM 9
           DGQ Q+M
Sbjct: 156 DGQRQLM 162



>NP_001243779
          Length = 34350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2     NDGQSQI 8
             NDG SQ+
Sbjct: 19651 NDGGSQV 19657



>NP_596869
          Length = 33423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2     NDGQSQI 8
             NDG SQ+
Sbjct: 18724 NDGGSQV 18730



>NP_001096078
          Length = 2263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    INDGQSQ 7
            + DGQSQ
Sbjct: 1109 LTDGQSQ 1115



>NP_004524
          Length = 1141

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NDGQSQIM 9
           +DG S+IM
Sbjct: 958 DDGNSEIM 965



>XP_005272296
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   DGQSQIM 9
           DGQ Q+M
Sbjct: 156 DGQRQLM 162



>XP_006717400
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   DGQSQIM 9
           DGQ Q+M
Sbjct: 156 DGQRQLM 162



>XP_006717399
          Length = 1002

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   DGQSQIM 9
           DGQ Q+M
Sbjct: 265 DGQRQLM 271



>XP_005272294
          Length = 1060

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   DGQSQIM 9
           DGQ Q+M
Sbjct: 265 DGQRQLM 271



>XP_005247178
          Length = 2263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    INDGQSQ 7
            + DGQSQ
Sbjct: 1109 LTDGQSQ 1115



>XP_005246888
          Length = 22663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    NDGQSQI 8
            NDG SQ+
Sbjct: 7964 NDGGSQV 7970



>XP_006712788
          Length = 24022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    NDGQSQI 8
            NDG SQ+
Sbjct: 9323 NDGGSQV 9329



>XP_006712787
          Length = 33978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2     NDGQSQI 8
             NDG SQ+
Sbjct: 19279 NDGGSQV 19285



>XP_006712786
          Length = 35484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2     NDGQSQI 8
             NDG SQ+
Sbjct: 20785 NDGGSQV 20791



>NP_001254479
          Length = 35991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2     NDGQSQI 8
             NDG SQ+
Sbjct: 21292 NDGGSQV 21298



>NP_597681
          Length = 27118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2     NDGQSQI 8
             NDG SQ+
Sbjct: 12419 NDGGSQV 12425



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.131    0.341 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 583,394
Number of extensions: 767
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)