Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1135
NP_671714                                                              18    1135
XP_006715890                                                           18    1135
XP_005250160                                                           18    1135
XP_005250159                                                           18    1135
NP_001257903                                                           17    4312
XP_006721269                                                           17    4312
NP_065988                                                              16    5631
NP_001135791                                                           16    5631
NP_079479                                                              16    5631
XP_005267986                                                           16    5631
NP_000391                                                              16    7355
NP_008971                                                              16    7355
NP_653333                                                              16    7355
NP_065856                                                              16    7355
XP_005258696                                                           16    7355
XP_005258523                                                           16    7355
XP_005258522                                                           16    7355
XP_005258520                                                           16    7355
XP_005258521                                                           16    7355
XP_005258519                                                           16    7355
XP_005258518                                                           16    7355
XP_005258516                                                           16    7355
XP_006722575                                                           16    7355
NP_004422                                                              15    9605
NP_055742                                                              15    9605
NP_001166126                                                           15    9605
NP_001162118                                                           15    9605
NP_055467                                                              15    9605
NP_060730                                                              15    9605
NP_006026                                                              15    9605
XP_005274525                                                           15    9605
XP_005268287                                                           15    9605
XP_005268286                                                           15    9605
XP_005268285                                                           15    9605
XP_005268284                                                           15    9605
XP_005245809                                                           15    9605
XP_005245808                                                           15    9605
XP_003846672                                                           15    9605
>NP_005742
          Length = 3907

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    INDEISVSS 9
            I+DEISVSS
Sbjct: 1565 IHDEISVSS 1573
>NP_671714
          Length = 3899

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    INDEISVSS 9
            I+DEISVSS
Sbjct: 1565 IHDEISVSS 1573
>XP_006715890
          Length = 3860

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    INDEISVSS 9
            I+DEISVSS
Sbjct: 1565 IHDEISVSS 1573
>XP_005250160
          Length = 3939

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    INDEISVSS 9
            I+DEISVSS
Sbjct: 1565 IHDEISVSS 1573
>XP_005250159
          Length = 3952

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    INDEISVSS 9
            I+DEISVSS
Sbjct: 1565 IHDEISVSS 1573
>NP_001257903
          Length = 5121

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    INDEISVSS 9
            I+DEI +SS
Sbjct: 1286 ISDEIKISS 1294
>XP_006721269
          Length = 5138

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    INDEISVSS 9
            I+DEI +SS
Sbjct: 1303 ISDEIKISS 1311
>NP_065988
          Length = 2048

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NDEISVSS 9
           NDE SVSS
Sbjct: 950 NDEKSVSS 957
>NP_001135791
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NDEISVSS 9
           N E+SVSS
Sbjct: 270 NSEVSVSS 277
>NP_079479
          Length = 721

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NDEISVSS 9
           N E+SVSS
Sbjct: 270 NSEVSVSS 277
>XP_005267986
          Length = 2022

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NDEISVSS 9
           NDE SVSS
Sbjct: 924 NDEKSVSS 931
>NP_000391
          Length = 760

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   NDEISVSS 9
           ND++++SS
Sbjct: 499 NDQVAISS 506
>NP_008971
          Length = 856

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   INDEISV 7
           IN+EISV
Sbjct: 263 INEEISV 269
>NP_653333
          Length = 756

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   INDEISV 7
           IN+EISV
Sbjct: 163 INEEISV 169
>NP_065856
          Length = 628

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   INDEISVSS 9
           +ND+ISV +
Sbjct: 177 LNDQISVQN 185
>XP_005258696
          Length = 736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   NDEISVSS 9
           ND++++SS
Sbjct: 475 NDQVAISS 482
>XP_005258523
          Length = 756

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   INDEISV 7
           IN+EISV
Sbjct: 163 INEEISV 169
>XP_005258522
          Length = 766

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   INDEISV 7
           IN+EISV
Sbjct: 163 INEEISV 169
>XP_005258520
          Length = 766

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   INDEISV 7
           IN+EISV
Sbjct: 163 INEEISV 169
>XP_005258521
          Length = 766

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   INDEISV 7
           IN+EISV
Sbjct: 163 INEEISV 169
>XP_005258519
          Length = 767

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   INDEISV 7
           IN+EISV
Sbjct: 174 INEEISV 180
>XP_005258518
          Length = 777

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   INDEISV 7
           IN+EISV
Sbjct: 174 INEEISV 180
>XP_005258516
          Length = 866

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   INDEISV 7
           IN+EISV
Sbjct: 263 INEEISV 269
>XP_006722575
          Length = 672

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   INDEISVSS 9
           +ND+ISV +
Sbjct: 221 LNDQISVQN 229
>NP_004422
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   DEISVSS 9
           DEI+VSS
Sbjct: 148 DEITVSS 154
>NP_055742
          Length = 1034

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    NDEISVSS 9
            ND +S+SS
Sbjct: 1007 NDPLSISS 1014
>NP_001166126
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   INDEISVSS 9
           IND  SVS+
Sbjct: 234 INDAFSVSA 242
>NP_001162118
          Length = 1004

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NDEISVSS 9
           ND +S+SS
Sbjct: 977 NDPLSISS 984
>NP_055467
          Length = 963

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NDEISVSS 9
           N E+SVSS
Sbjct: 540 NPELSVSS 547
>NP_060730
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   INDEISVSS 9
           IND  SVS+
Sbjct: 168 INDAFSVSA 176
>NP_006026
          Length = 1711

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NDEISVSS 9
            +DE SVSS
Sbjct: 1151 DDEFSVSS 1158
>XP_005274525
          Length = 985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NDEISVSS 9
           ND +S+SS
Sbjct: 958 NDPLSISS 965
>XP_005268287
          Length = 1490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NDEISVSS 9
            +DE SVSS
Sbjct: 1168 DDEFSVSS 1175
>XP_005268286
          Length = 1685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NDEISVSS 9
            +DE SVSS
Sbjct: 1125 DDEFSVSS 1132
>XP_005268285
          Length = 1702

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NDEISVSS 9
            +DE SVSS
Sbjct: 1142 DDEFSVSS 1149
>XP_005268284
          Length = 1728

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NDEISVSS 9
            +DE SVSS
Sbjct: 1168 DDEFSVSS 1175
>XP_005245809
          Length = 922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   DEISVSS 9
           DEI+VSS
Sbjct: 94  DEITVSS 100
>XP_005245808
          Length = 965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   DEISVSS 9
           DEI+VSS
Sbjct: 137 DEITVSS 143
>XP_003846672
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NDEISVSS 9
           N E+SVSS
Sbjct: 540 NPELSVSS 547
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.116    0.259 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 461,706
Number of extensions: 641
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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