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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              19     665
NP_060240                                                              17    3301
NP_068586                                                              17    4312
NP_001229757                                                           17    4312
XP_005249324                                                           17    4312
XP_005249323                                                           17    4312
NP_073563                                                              16    5631
NP_001098028                                                           16    5631
NP_079206                                                              16    5631
XP_005248664                                                           16    5631
XP_005248663                                                           16    5631
XP_006714758                                                           16    5631
NP_004659                                                              16    7355
XP_006716232                                                           16    7355
XP_006716231                                                           16    7355
NP_996991                                                              15    9605
NP_001273093                                                           15    9605
NP_004310                                                              15    9605
NP_001161807                                                           15    9605
NP_002387                                                              15    9605
NP_060147                                                              15    9605
NP_064593                                                              15    9605
NP_001271279                                                           15    9605
XP_005265293                                                           15    9605
NP_071800                                                              15    9605
NP_001025190                                                           15    9605
NP_689995                                                              15    9605

>NP_000312
          Length = 928

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           IMM SMYGI
Sbjct: 703 IMMCSMYGI 711



>NP_060240
          Length = 296

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           I+M+S YG+
Sbjct: 181 ILMYSYYGL 189



>NP_068586
          Length = 299

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           ++M+S YG+
Sbjct: 178 VLMYSYYGL 186



>NP_001229757
          Length = 326

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           ++M+S YG+
Sbjct: 205 VLMYSYYGL 213



>XP_005249324
          Length = 299

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           ++M+S YG+
Sbjct: 178 VLMYSYYGL 186



>XP_005249323
          Length = 299

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           ++M+S YG+
Sbjct: 178 VLMYSYYGL 186



>NP_073563
          Length = 314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           ++M+S YG+
Sbjct: 193 VIMYSYYGL 201



>NP_001098028
          Length = 281

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           ++M+S YG+
Sbjct: 182 VVMYSYYGL 190



>NP_079206
          Length = 281

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           ++M+S YG+
Sbjct: 182 VVMYSYYGL 190



>XP_005248664
          Length = 268

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           ++M+S YG+
Sbjct: 169 VVMYSYYGL 177



>XP_005248663
          Length = 281

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           ++M+S YG+
Sbjct: 182 VVMYSYYGL 190



>XP_006714758
          Length = 281

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IMMFSMYGI 9
           ++M+S YG+
Sbjct: 182 VVMYSYYGL 190



>NP_004659
          Length = 1857

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IMMFSMYGI 9
            +M FS++GI
Sbjct: 1541 MMEFSLFGI 1549



>XP_006716232
          Length = 2753

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IMMFSMYGI 9
            +M FS++GI
Sbjct: 1541 MMEFSLFGI 1549




 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IMMFSMYGI 9
            +M FS++GI
Sbjct: 2437 MMEFSLFGI 2445



>XP_006716231
          Length = 2753

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IMMFSMYGI 9
            +M FS++GI
Sbjct: 1541 MMEFSLFGI 1549




 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IMMFSMYGI 9
            +M FS++GI
Sbjct: 2437 MMEFSLFGI 2445



>NP_996991
          Length = 1216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    IMMFSMYGI 9
            I MF+++G+
Sbjct: 1119 IYMFTLWGV 1127



>NP_001273093
          Length = 1228

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    IMMFSMYGI 9
            I MF+++G+
Sbjct: 1119 IYMFTLWGV 1127



>NP_004310
          Length = 1294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    IMMFSMYGI 9
            I MF+++G+
Sbjct: 1119 IYMFTLWGV 1127



>NP_001161807
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   IMMFSMYGI 9
           I MF  YG+
Sbjct: 341 IWMFDKYGL 349



>NP_002387
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   IMMFSMYGI 9
           I MF  YG+
Sbjct: 341 IWMFDKYGL 349



>NP_060147
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   FSMYGI 9
           F+MYG+
Sbjct: 478 FTMYGV 483



>NP_064593
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IMMFSMYG 8
           I+ FS+YG
Sbjct: 292 IVTFSIYG 299



>NP_001271279
          Length = 345

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FSMYGI 9
           FS+YGI
Sbjct: 272 FSVYGI 277



>XP_005265293
          Length = 547

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IMMFSMYG 8
           I+ FS+YG
Sbjct: 292 IVTFSIYG 299



>NP_071800
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IMMFSMYG 8
           I+ FS+YG
Sbjct: 255 IVTFSIYG 262



>NP_001025190
          Length = 357

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FSMYGI 9
           FS+YGI
Sbjct: 284 FSVYGI 289



>NP_689995
          Length = 357

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FSMYGI 9
           FS+YGI
Sbjct: 284 FSVYGI 289



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.353    0.154    0.464 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 329,345
Number of extensions: 521
Number of successful extensions: 29
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)