Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_056030                                                              18    1935
NP_060024                                                              17    4312
NP_000135                                                              16    5631
NP_061841                                                              16    5631
XP_006711953                                                           16    5631
XP_006711952                                                           16    5631
XP_006711951                                                           16    5631
NP_004175                                                              16    7355
NP_776049                                                              16    7355
NP_998811                                                              16    7355
NP_998810                                                              16    7355
XP_006720312                                                           16    7355
XP_005268101                                                           16    7355
XP_006720311                                                           16    7355
XP_006719294                                                           16    7355
NP_001138947                                                           16    7355
NP_056082                                                              15    9605
NP_000892                                                              15    9605
NP_002102                                                              15    9605
NP_001159576                                                           15    9605
NP_001189472                                                           15    9605
NP_061198                                                              15    9605
NP_001008727                                                           15    9605
NP_853530                                                              15    9605
NP_056299                                                              15    9605
NP_110401                                                              15    9605
XP_005260121                                                           15    9605
XP_005260119                                                           15    9605
XP_006720670                                                           15    9605
XP_006719362                                                           15    9605
XP_006715919                                                           15    9605
XP_006715917                                                           15    9605
XP_005250208                                                           15    9605
XP_005250207                                                           15    9605
XP_006714285                                                           15    9605
XP_005263071                                                           15    9605
NP_997053                                                              15    9605

>NP_056030
          Length = 1673

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   ILTKQIKTK 9
           +LTK+IKT+
Sbjct: 527 VLTKEIKTE 535



>NP_060024
          Length = 1801

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ILTKQIKTK 9
           +LTK+ KTK
Sbjct: 104 LLTKESKTK 112



>NP_000135
          Length = 210

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTKQIKTK 9
           LTK +KTK
Sbjct: 190 LTKALKTK 197



>NP_061841
          Length = 539

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TKQIKTK 9
           TKQI+TK
Sbjct: 490 TKQIETK 496



>XP_006711953
          Length = 314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TKQIKTK 9
           TKQI+TK
Sbjct: 265 TKQIETK 271



>XP_006711952
          Length = 453

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TKQIKTK 9
           TKQI+TK
Sbjct: 404 TKQIETK 410



>XP_006711951
          Length = 480

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TKQIKTK 9
           TKQI+TK
Sbjct: 431 TKQIETK 437



>NP_004175
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQIKTK 9
           KQIKTK
Sbjct: 366 KQIKTK 371



>NP_776049
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQIKTK 9
           KQIKTK
Sbjct: 366 KQIKTK 371



>NP_998811
          Length = 430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQIKTK 9
           KQIKTK
Sbjct: 325 KQIKTK 330



>NP_998810
          Length = 430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQIKTK 9
           KQIKTK
Sbjct: 325 KQIKTK 330



>XP_006720312
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQIKTK 9
           KQIKTK
Sbjct: 366 KQIKTK 371



>XP_005268101
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQIKTK 9
           KQIKTK
Sbjct: 366 KQIKTK 371



>XP_006720311
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQIKTK 9
           KQIKTK
Sbjct: 366 KQIKTK 371



>XP_006719294
          Length = 2348

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTKQIKTK 9
           L +QIKTK
Sbjct: 978 LERQIKTK 985




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LTKQIKT 8
           LTKQ+K+
Sbjct: 630 LTKQVKS 636



>NP_001138947
          Length = 2351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTKQIKTK 9
           L +QIKTK
Sbjct: 978 LERQIKTK 985




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LTKQIKT 8
           LTKQ+K+
Sbjct: 630 LTKQVKS 636



>NP_056082
          Length = 1486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LTKQIKTK 9
           LT+Q+K K
Sbjct: 901 LTRQVKEK 908



>NP_000892
          Length = 984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  ILTKQIKTK 9
          ILT  IKT+
Sbjct: 83 ILTSDIKTE 91



>NP_002102
          Length = 3144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ILTKQIKTK 9
            I+TKQ+K +
Sbjct: 1757 IVTKQLKVE 1765



>NP_001159576
          Length = 867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  ILTKQIKTK 9
          ILT  IKT+
Sbjct: 83 ILTSDIKTE 91



>NP_001189472
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LTKQIKTK 9
           LT+Q+K K
Sbjct: 959 LTRQVKEK 966



>NP_061198
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    LTKQIKT 8
            L+KQ+KT
Sbjct: 1064 LSKQVKT 1070



>NP_001008727
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ILTKQIKT 8
           +LTK +KT
Sbjct: 382 LLTKHLKT 389



>NP_853530
          Length = 1505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LTKQIKTK 9
           LT+Q+K K
Sbjct: 948 LTRQVKEK 955



>NP_056299
          Length = 243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LTKQIK 7
           LTKQIK
Sbjct: 138 LTKQIK 143



>NP_110401
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TKQIKTK 9
           TKQI+T+
Sbjct: 295 TKQIRTR 301



>XP_005260121
          Length = 377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ILTKQIKT 8
           +LTK +KT
Sbjct: 369 LLTKHLKT 376



>XP_005260119
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ILTKQIKT 8
           +LTK +KT
Sbjct: 382 LLTKHLKT 389



>XP_006720670
          Length = 1712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    LTKQIKT 8
            L+KQ+KT
Sbjct: 1064 LSKQVKT 1070



>XP_006719362
          Length = 1546

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LTKQIKTK 9
           LT+Q+K K
Sbjct: 961 LTRQVKEK 968



>XP_006715919
          Length = 1494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LTKQIKTK 9
           LT+Q+K K
Sbjct: 937 LTRQVKEK 944



>XP_006715917
          Length = 1583

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LTKQIKTK 9
            LT+Q+K K
Sbjct: 1026 LTRQVKEK 1033



>XP_005250208
          Length = 1603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LTKQIKTK 9
            LT+Q+K K
Sbjct: 1046 LTRQVKEK 1053



>XP_005250207
          Length = 1604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LTKQIKTK 9
            LT+Q+K K
Sbjct: 1047 LTRQVKEK 1054



>XP_006714285
          Length = 984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  ILTKQIKTK 9
          ILT  IKT+
Sbjct: 83 ILTSDIKTE 91



>XP_005263071
          Length = 984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  ILTKQIKTK 9
          ILT  IKT+
Sbjct: 83 ILTSDIKTE 91



>NP_997053
          Length = 201

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LTKQIK 7
           LTKQIK
Sbjct: 96  LTKQIK 101



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.129    0.302 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 362,073
Number of extensions: 709
Number of successful extensions: 39
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)