Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              19     869
NP_000069                                                              17    2528
NP_001273014                                                           17    2528
XP_006721187                                                           17    2528
NP_005678                                                              16    5631
XP_006712233                                                           16    5631
XP_006712232                                                           16    5631
XP_006712231                                                           16    5631
NP_733751                                                              16    7355
NP_079092                                                              16    7355
NP_001793                                                              16    7355
NP_061124                                                              16    7355
XP_005276559                                                           16    7355
XP_005253478                                                           16    7355
XP_006716142                                                           16    7355
XP_005250088                                                           16    7355
XP_006716141                                                           16    7355
XP_005250085                                                           16    7355
XP_006716140                                                           16    7355
XP_005250084                                                           16    7355
XP_005250083                                                           16    7355
XP_005250082                                                           16    7355
NP_775797                                                              15    9605
NP_001276018                                                           15    9605
NP_079048                                                              15    9605
NP_001276019                                                           15    9605
NP_001276017                                                           15    9605
NP_001276020                                                           15    9605
NP_057506                                                              15    9605
XP_006721915                                                           15    9605
XP_006720124                                                           15    9605
XP_005267429                                                           15    9605
XP_006720123                                                           15    9605
XP_005271279                                                           15    9605
XP_005271277                                                           15    9605
XP_005271276                                                           15    9605
XP_005271275                                                           15    9605
XP_005271273                                                           15    9605
NP_055418                                                              15    9605
>NP_002430
          Length = 1137

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILNEQTAKV 9
           ILNEQ AKV
Sbjct: 688 ILNEQAAKV 696
>NP_000069
          Length = 493

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ILNEQTAKV 9
          +LN +TAKV
Sbjct: 39 VLNHETAKV 47
>NP_001273014
          Length = 433

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ILNEQTAKV 9
          +LN +TAKV
Sbjct: 39 VLNHETAKV 47
>XP_006721187
          Length = 266

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ILNEQTAKV 9
          +LN +TAKV
Sbjct: 39 VLNHETAKV 47
>NP_005678
          Length = 589

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ILNEQTAKV 9
           I+ E+TAK+
Sbjct: 109 IITEETAKI 117
>XP_006712233
          Length = 490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ILNEQTAKV 9
          I+ E+TAK+
Sbjct: 10 IITEETAKI 18
>XP_006712232
          Length = 490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ILNEQTAKV 9
          I+ E+TAK+
Sbjct: 10 IITEETAKI 18
>XP_006712231
          Length = 490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ILNEQTAKV 9
          I+ E+TAK+
Sbjct: 10 IITEETAKI 18
>NP_733751
          Length = 4911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ILNEQTAK 8
            +++EQTAK
Sbjct: 1989 VVSEQTAK 1996
>NP_079092
          Length = 569

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ILNEQTAKV 9
           +L+EQ AKV
Sbjct: 503 LLSEQRAKV 511
>NP_001793
          Length = 454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LNEQTAKV 9
          +NEQ AKV
Sbjct: 76 MNEQHAKV 83
>NP_061124
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LNEQTAKV 9
          LN+QTA V
Sbjct: 84 LNQQTAPV 91
>XP_005276559
          Length = 454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LNEQTAKV 9
          +NEQ AKV
Sbjct: 76 MNEQHAKV 83
>XP_005253478
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LNEQTAKV 9
          LN+QTA V
Sbjct: 84 LNQQTAPV 91
>XP_006716142
          Length = 4927

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ILNEQTAK 8
            +++EQTAK
Sbjct: 2006 VVSEQTAK 2013
>XP_005250088
          Length = 4928

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ILNEQTAK 8
            +++EQTAK
Sbjct: 2006 VVSEQTAK 2013
>XP_006716141
          Length = 4959

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ILNEQTAK 8
            +++EQTAK
Sbjct: 2006 VVSEQTAK 2013
>XP_005250085
          Length = 4979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ILNEQTAK 8
            +++EQTAK
Sbjct: 2006 VVSEQTAK 2013
>XP_006716140
          Length = 4982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ILNEQTAK 8
            +++EQTAK
Sbjct: 2006 VVSEQTAK 2013
>XP_005250084
          Length = 4982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ILNEQTAK 8
            +++EQTAK
Sbjct: 2006 VVSEQTAK 2013
>XP_005250083
          Length = 4982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ILNEQTAK 8
            +++EQTAK
Sbjct: 2005 VVSEQTAK 2012
>XP_005250082
          Length = 4983

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ILNEQTAK 8
            +++EQTAK
Sbjct: 2006 VVSEQTAK 2013
>NP_775797
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ILNEQTAKV 9
           +LN++ AKV
Sbjct: 173 LLNQEKAKV 181
>NP_001276018
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNEQTAK 8
           LNE TAK
Sbjct: 986 LNETTAK 992
>NP_079048
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    LNEQTAK 8
            LNE TAK
Sbjct: 1005 LNETTAK 1011
>NP_001276019
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    LNEQTAK 8
            LNE TAK
Sbjct: 1005 LNETTAK 1011
>NP_001276017
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    LNEQTAK 8
            LNE TAK
Sbjct: 1005 LNETTAK 1011
>NP_001276020
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNEQTAK 8
           LNE TAK
Sbjct: 645 LNETTAK 651
>NP_057506
          Length = 230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LNEQTAKV 9
          LN+ TAKV
Sbjct: 45 LNDVTAKV 52
>XP_006721915
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LNEQTAKV 9
          +NEQ AKV
Sbjct: 76 VNEQHAKV 83
>XP_006720124
          Length = 339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ILNEQTAKV 9
          +LN++ AKV
Sbjct: 87 LLNQEKAKV 95
>XP_005267429
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ILNEQTAKV 9
           +LN++ AKV
Sbjct: 173 LLNQEKAKV 181
>XP_006720123
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ILNEQTAKV 9
           +LN++ AKV
Sbjct: 173 LLNQEKAKV 181
>XP_005271279
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNEQTAK 8
           LNE TAK
Sbjct: 645 LNETTAK 651
>XP_005271277
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNEQTAK 8
           LNE TAK
Sbjct: 814 LNETTAK 820
>XP_005271276
          Length = 1067

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNEQTAK 8
           LNE TAK
Sbjct: 930 LNETTAK 936
>XP_005271275
          Length = 1067

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNEQTAK 8
           LNE TAK
Sbjct: 930 LNETTAK 936
>XP_005271273
          Length = 1130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNEQTAK 8
           LNE TAK
Sbjct: 993 LNETTAK 999
>NP_055418
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LNEQTAKV 9
          +NEQ AKV
Sbjct: 76 VNEQHAKV 83
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.121    0.284 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 341,440
Number of extensions: 287
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package