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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_006719760                                                           17    4312
XP_006719759                                                           17    4312
XP_006719758                                                           17    4312
XP_005254048                                                           17    4312
XP_006719757                                                           17    4312
NP_997194                                                              17    4312
NP_001138473                                                           16    5631
NP_001159643                                                           16    5631
NP_001159639                                                           16    5631
NP_001128504                                                           16    5631
NP_037488                                                              16    5631
NP_001159641                                                           16    5631
XP_006716998                                                           16    5631
XP_006716997                                                           16    5631
NP_115557                                                              16    5631
NP_001367                                                              16    7355
NP_001184154                                                           16    7355
XP_006721207                                                           16    7355
NP_001004747                                                           16    7355
NP_001004477                                                           16    7355
NP_009224                                                              15    9605
NP_001239133                                                           15    9605
NP_001002029                                                           15    9605
NP_775733                                                              15    9605
NP_982288                                                              15    9605
NP_001229752                                                           15    9605
XP_005268242                                                           15    9605
XP_005268241                                                           15    9605
XP_005246497                                                           15    9605
XP_005270390                                                           15    9605
XP_006711823                                                           15    9605
NP_733843                                                              15    9605
NP_001243055                                                           15    9605

>XP_006719760
          Length = 504

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRLSCR 9
           VRLSCR
Sbjct: 318 VRLSCR 323



>XP_006719759
          Length = 577

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRLSCR 9
           VRLSCR
Sbjct: 318 VRLSCR 323



>XP_006719758
          Length = 578

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRLSCR 9
           VRLSCR
Sbjct: 318 VRLSCR 323



>XP_005254048
          Length = 621

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRLSCR 9
           VRLSCR
Sbjct: 314 VRLSCR 319



>XP_006719757
          Length = 625

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRLSCR 9
           VRLSCR
Sbjct: 318 VRLSCR 323



>NP_997194
          Length = 573

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRLSCR 9
           VRLSCR
Sbjct: 314 VRLSCR 319



>NP_001138473
          Length = 347

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IVRLSCR 9
          +VRL+CR
Sbjct: 28 LVRLACR 34



>NP_001159643
          Length = 347

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IVRLSCR 9
          +VRL+CR
Sbjct: 28 LVRLACR 34



>NP_001159639
          Length = 331

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IVRLSCR 9
          +VRL+CR
Sbjct: 12 LVRLACR 18



>NP_001128504
          Length = 152

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  LIVRLSC 8
          L++R+SC
Sbjct: 53 LVIRISC 59



>NP_037488
          Length = 504

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  ILIVRLSCR 9
          IL+ R  CR
Sbjct: 77 ILVTRFGCR 85



>NP_001159641
          Length = 338

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IVRLSCR 9
          +VRL+CR
Sbjct: 19 LVRLACR 25



>XP_006716998
          Length = 357

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IVRLSCR 9
          +VRL+CR
Sbjct: 38 LVRLACR 44



>XP_006716997
          Length = 371

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IVRLSCR 9
          +VRL+CR
Sbjct: 52 LVRLACR 58



>NP_115557
          Length = 152

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  LIVRLSC 8
          L++R+SC
Sbjct: 53 LVIRISC 59



>NP_001367
          Length = 4646

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    IVRLSC 8
            +VRLSC
Sbjct: 3935 VVRLSC 3940



>NP_001184154
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ILIVRLSC 8
           I +VR+SC
Sbjct: 103 IFLVRVSC 110



>XP_006721207
          Length = 159

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  ILIVRLSC 8
          I +VR+SC
Sbjct: 54 IFLVRVSC 61



>NP_001004747
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ILIVRLSC 8
           ILIV +SC
Sbjct: 241 ILIVLISC 248



>NP_001004477
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   ILIVRLSC 8
           + ++RLSC
Sbjct: 198 LAVIRLSC 205



>NP_009224
          Length = 1744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    IVRLSCR 9
            +VR SCR
Sbjct: 1668 LVRASCR 1674



>NP_001239133
          Length = 1698

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    IVRLSCR 9
            +VR SCR
Sbjct: 1622 LVRASCR 1628



>NP_001002029
          Length = 1744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    IVRLSCR 9
            +VR SCR
Sbjct: 1668 LVRASCR 1674



>NP_775733
          Length = 1251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   IVRLSC 8
           +VRLSC
Sbjct: 899 LVRLSC 904



>NP_982288
          Length = 384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   LIVRLSCR 9
           LIV+++C+
Sbjct: 321 LIVKIACK 328



>NP_001229752
          Length = 1744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    IVRLSCR 9
            +VR SCR
Sbjct: 1668 LVRASCR 1674



>XP_005268242
          Length = 1285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   IVRLSC 8
           +VRLSC
Sbjct: 933 LVRLSC 938



>XP_005268241
          Length = 1300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   IVRLSC 8
           +VRLSC
Sbjct: 948 LVRLSC 953



>XP_005246497
          Length = 200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   LIVRLSCR 9
           LIV+++C+
Sbjct: 129 LIVKIACK 136



>XP_005270390
          Length = 589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   LIVRLSC 8
           LI+R++C
Sbjct: 245 LIIRMNC 251



>XP_006711823
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   LIVRLSC 8
           LI+R++C
Sbjct: 248 LIIRMNC 254



>NP_733843
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   LIVRLSC 8
           LI+R++C
Sbjct: 248 LIIRMNC 254



>NP_001243055
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   LIVRLSCR 9
           LIV+++C+
Sbjct: 321 LIVKIACK 328



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.354    0.159    0.488 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 492,728
Number of extensions: 668
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)