Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           19     869
NP_036462                                                              19     869
NP_001243397                                                           19     869
XP_005269721                                                           19     869
NP_003048                                                              17    2528
NP_694857                                                              17    2528
XP_006726853                                                           17    2528
XP_006723596                                                           17    2528
XP_005267162                                                           17    2528
XP_005267161                                                           17    2528
XP_006715615                                                           17    2528
XP_005267160                                                           17    2528
XP_005267159                                                           17    2528
XP_006710111                                                           17    2528
NP_001070650                                                           17    3301
NP_036550                                                              17    3301
NP_079366                                                              17    3301
XP_005257601                                                           17    3301
XP_005257599                                                           17    3301
XP_005257600                                                           17    3301
XP_005257253                                                           17    3301
XP_005257252                                                           17    3301
NP_056507                                                              17    4312
NP_758441                                                              17    4312
NP_525022                                                              16    5631
NP_001275915                                                           16    5631
NP_001275914                                                           16    5631
NP_009099                                                              16    5631
NP_525021                                                              16    5631
XP_005276813                                                           16    5631
XP_005256997                                                           16    5631
XP_005256995                                                           16    5631
XP_005256993                                                           16    5631
XP_005256992                                                           16    5631
XP_006721697                                                           16    5631
XP_006721696                                                           16    5631
XP_005256991                                                           16    5631
XP_005248455                                                           16    5631
NP_001790                                                              16    5631
NP_001119516                                                           16    7355
NP_942012                                                              16    7355
NP_942008                                                              16    7355
NP_942007                                                              16    7355
NP_942009                                                              16    7355
NP_942011                                                              16    7355
NP_942010                                                              16    7355
NP_001181988                                                           16    7355
NP_001010893                                                           16    7355
NP_079477                                                              16    7355
NP_001230913                                                           15    9605
NP_068810                                                              15    9605
NP_001138610                                                           15    9605
NP_001230914                                                           15    9605
NP_001009                                                              15    9605
NP_003260                                                              15    9605
NP_001273031                                                           15    9605
XP_006712357                                                           15    9605
XP_006712356                                                           15    9605
XP_006712355                                                           15    9605
NP_000982                                                              15    9605
>NP_001243398
          Length = 1781

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IKRKRTFVL 9
           IKRKR FVL
Sbjct: 829 IKRKRPFVL 837
>NP_036462
          Length = 2073

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IKRKRTFVL 9
            IKRKR FVL
Sbjct: 1121 IKRKRPFVL 1129
>NP_001243397
          Length = 1890

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IKRKRTFVL 9
           IKRKR FVL
Sbjct: 938 IKRKRPFVL 946
>XP_005269721
          Length = 2073

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IKRKRTFVL 9
            IKRKR FVL
Sbjct: 1121 IKRKRPFVL 1129
>NP_003048
          Length = 554

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RKRTFVL 9
           RKRTF+L
Sbjct: 344 RKRTFIL 350
>NP_694857
          Length = 506

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RKRTFVL 9
           RKRTF+L
Sbjct: 344 RKRTFIL 350
>XP_006726853
          Length = 707

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IKRKRTFV 8
           +KR+RTFV
Sbjct: 222 LKRQRTFV 229
>XP_006723596
          Length = 707

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IKRKRTFV 8
           +KR+RTFV
Sbjct: 222 LKRQRTFV 229
>XP_005267162
          Length = 418

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RKRTFVL 9
           RKRTF+L
Sbjct: 152 RKRTFIL 158
>XP_005267161
          Length = 418

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RKRTFVL 9
           RKRTF+L
Sbjct: 152 RKRTFIL 158
>XP_006715615
          Length = 483

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RKRTFVL 9
           RKRTF+L
Sbjct: 344 RKRTFIL 350
>XP_005267160
          Length = 573

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RKRTFVL 9
           RKRTF+L
Sbjct: 344 RKRTFIL 350
>XP_005267159
          Length = 610

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RKRTFVL 9
           RKRTF+L
Sbjct: 344 RKRTFIL 350
>XP_006710111
          Length = 566

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  IKRKRTFV 8
          +KR+RTFV
Sbjct: 81 LKRQRTFV 88
>NP_001070650
          Length = 516

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  IKRKRTFVL 9
          +KRK+T+VL
Sbjct: 40 LKRKQTWVL 48
>NP_036550
          Length = 492

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  IKRKRTFVL 9
          +KRK+T+VL
Sbjct: 40 LKRKQTWVL 48
>NP_079366
          Length = 1123

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KRKRTFV 8
           KRK+TFV
Sbjct: 811 KRKKTFV 817
>XP_005257601
          Length = 1123

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KRKRTFV 8
           KRK+TFV
Sbjct: 811 KRKKTFV 817
>XP_005257599
          Length = 1123

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KRKRTFV 8
           KRK+TFV
Sbjct: 811 KRKKTFV 817
>XP_005257600
          Length = 1123

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KRKRTFV 8
           KRK+TFV
Sbjct: 811 KRKKTFV 817
>XP_005257253
          Length = 492

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  IKRKRTFVL 9
          +KRK+T+VL
Sbjct: 40 LKRKQTWVL 48
>XP_005257252
          Length = 516

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  IKRKRTFVL 9
          +KRK+T+VL
Sbjct: 40 LKRKQTWVL 48
>NP_056507
          Length = 1932

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KRKRTF 7
           KRKRTF
Sbjct: 788 KRKRTF 793
>NP_758441
          Length = 745

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IKRKRTF 7
           IK+KRTF
Sbjct: 210 IKQKRTF 216
>NP_525022
          Length = 1624

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1425 IKRKLCFVL 1433
>NP_001275915
          Length = 1616

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1417 IKRKLCFVL 1425
>NP_001275914
          Length = 1621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1422 IKRKLCFVL 1430
>NP_009099
          Length = 1581

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1382 IKRKLCFVL 1390
>NP_525021
          Length = 1543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1344 IKRKLCFVL 1352
>XP_005276813
          Length = 289

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   IKRKRTFVL 9
           IKRKRT  L
Sbjct: 270 IKRKRTEAL 278
>XP_005256997
          Length = 1560

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1361 IKRKLCFVL 1369
>XP_005256995
          Length = 1621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1422 IKRKLCFVL 1430
>XP_005256993
          Length = 1543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1344 IKRKLCFVL 1352
>XP_005256992
          Length = 1543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1344 IKRKLCFVL 1352
>XP_006721697
          Length = 1625

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1433 IKRKLCFVL 1441
>XP_006721696
          Length = 1632

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1433 IKRKLCFVL 1441
>XP_005256991
          Length = 1632

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    IKRKRTFVL 9
            IKRK  FVL
Sbjct: 1433 IKRKLCFVL 1441
>XP_005248455
          Length = 289

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   IKRKRTFVL 9
           IKRKRT  L
Sbjct: 270 IKRKRTEAL 278
>NP_001790
          Length = 346

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   IKRKRTFVL 9
           IKRKRT  L
Sbjct: 327 IKRKRTEAL 335
>NP_001119516
          Length = 390

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KRKRTFVL 9
          KRK++F+L
Sbjct: 83 KRKKSFLL 90
>NP_942012
          Length = 390

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KRKRTFVL 9
          KRK++F+L
Sbjct: 83 KRKKSFLL 90
>NP_942008
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KRKRTFVL 9
          KRK++F+L
Sbjct: 83 KRKKSFLL 90
>NP_942007
          Length = 390

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KRKRTFVL 9
          KRK++F+L
Sbjct: 83 KRKKSFLL 90
>NP_942009
          Length = 374

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KRKRTFVL 9
          KRK++F+L
Sbjct: 83 KRKKSFLL 90
>NP_942011
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KRKRTFVL 9
          KRK++F+L
Sbjct: 83 KRKKSFLL 90
>NP_942010
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KRKRTFVL 9
          KRK++F+L
Sbjct: 83 KRKKSFLL 90
>NP_001181988
          Length = 440

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  IKRKRTFVL 9
          +KRK  F+L
Sbjct: 28 LKRKHRFIL 36
>NP_001010893
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KRKRTFVL 9
           KRKR F++
Sbjct: 431 KRKRNFLI 438
>NP_079477
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  IKRKRTFVL 9
          +KRK  F+L
Sbjct: 78 LKRKHRFIL 86
>NP_001230913
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KRKRTF 7
           KRKRT+
Sbjct: 301 KRKRTY 306
>NP_068810
          Length = 551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KRKRTF 7
           KRKRT+
Sbjct: 301 KRKRTY 306
>NP_001138610
          Length = 548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KRKRTF 7
           KRKRT+
Sbjct: 298 KRKRTY 303
>NP_001230914
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KRKRTF 7
           KRKRT+
Sbjct: 301 KRKRTY 306
>NP_001009
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  KRKRTF 7
          K+KRTF
Sbjct: 7  KKKRTF 12
>NP_003260
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  IKRKRTFV 8
          I+R RT+V
Sbjct: 44 IRRNRTYV 51
>NP_001273031
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  IKRKRTFV 8
          I+R RT+V
Sbjct: 81 IRRNRTYV 88
>XP_006712357
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RKRTFVL 9
           +K+TFVL
Sbjct: 183 KKKTFVL 189
>XP_006712356
          Length = 303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RKRTFVL 9
           +K+TFVL
Sbjct: 182 KKKTFVL 188
>XP_006712355
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RKRTFVL 9
           +K+TFVL
Sbjct: 183 KKKTFVL 189
>NP_000982
          Length = 137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   IKRKRT 6
           +KRKRT
Sbjct: 126 VKRKRT 131
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.346    0.153    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 497,547
Number of extensions: 740
Number of successful extensions: 60
Number of sequences better than 10000.0: 60
Number of HSP's gapped: 60
Number of HSP's successfully gapped: 60
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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