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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_114122                                                              17    3301
NP_001188395                                                           17    3301
NP_689648                                                              17    3301
XP_006714567                                                           17    3301
XP_006714566                                                           17    3301
NP_001075                                                              17    4312
XP_005259906                                                           17    4312
NP_057308                                                              16    5631
XP_005267072                                                           16    5631
NP_037453                                                              16    7355
NP_001106197                                                           15    9605
NP_055820                                                              15    9605
NP_116184                                                              15    9605
NP_078988                                                              15    9605
NP_001138299                                                           15    9605
XP_005267607                                                           15    9605

>NP_114122
          Length = 230

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  IKMRMEMP 8
          +K RMEMP
Sbjct: 57 VKDRMEMP 64



>NP_001188395
          Length = 230

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  IKMRMEMP 8
          +K RMEMP
Sbjct: 57 VKDRMEMP 64



>NP_689648
          Length = 426

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IKMRMEMPQ 9
           + MRM MP+
Sbjct: 353 VNMRMSMPE 361



>XP_006714567
          Length = 257

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  IKMRMEMP 8
          +K RMEMP
Sbjct: 57 VKDRMEMP 64



>XP_006714566
          Length = 257

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  IKMRMEMP 8
          +K RMEMP
Sbjct: 57 VKDRMEMP 64



>NP_001075
          Length = 738

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MRMEMPQ 9
           +RME+PQ
Sbjct: 472 LRMELPQ 478



>XP_005259906
          Length = 206

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KMRMEMPQ 9
           K+R E+PQ
Sbjct: 168 KLRQELPQ 175



>NP_057308
          Length = 595

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   IKMRMEMPQ 9
           IKMRME  Q
Sbjct: 324 IKMRMERLQ 332



>XP_005267072
          Length = 588

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   IKMRMEMPQ 9
           IKMRME  Q
Sbjct: 317 IKMRMERLQ 325



>NP_037453
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  RMEMPQ 9
          RM+MPQ
Sbjct: 53 RMQMPQ 58



>NP_001106197
          Length = 1554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IKMRMEMPQ 9
           ++MR  +PQ
Sbjct: 695 VRMRRRLPQ 703



>NP_055820
          Length = 1519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IKMRMEMPQ 9
           ++MR  +PQ
Sbjct: 625 VRMRRRLPQ 633



>NP_116184
          Length = 377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   KMRMEMP 8
           ++R+EMP
Sbjct: 355 RLRLEMP 361



>NP_078988
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  IKMRMEMPQ 9
          IKMR  +PQ
Sbjct: 65 IKMRDAIPQ 73



>NP_001138299
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   KMRMEMP 8
           ++R+EMP
Sbjct: 324 RLRLEMP 330



>XP_005267607
          Length = 1589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IKMRMEMPQ 9
           ++MR  +PQ
Sbjct: 695 VRMRRRLPQ 703



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.133    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 515,980
Number of extensions: 685
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)