Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              19     869
NP_001006619                                                           17    3301
NP_001006618                                                           17    3301
NP_001006620                                                           17    3301
NP_077022                                                              17    3301
NP_001006621                                                           17    3301
NP_001006622                                                           17    3301
NP_001888                                                              16    5631
NP_114418                                                              16    5631
NP_001116451                                                           16    5631
NP_001164102                                                           16    5631
XP_005273343                                                           16    5631
NP_003310                                                              16    7355
NP_597676                                                              16    7355
NP_002200                                                              16    7355
NP_003090                                                              16    7355
NP_001243779                                                           16    7355
NP_001107852                                                           16    7355
NP_001229862                                                           16    7355
NP_596869                                                              16    7355
NP_683758                                                              16    7355
NP_003881                                                              16    7355
NP_065764                                                              16    7355
XP_005260283                                                           16    7355
XP_006721107                                                           16    7355
XP_005255370                                                           16    7355
XP_005254668                                                           16    7355
XP_005254667                                                           16    7355
XP_005246888                                                           16    7355
XP_006712788                                                           16    7355
XP_006712787                                                           16    7355
XP_006712786                                                           16    7355
NP_001254479                                                           16    7355
NP_597681                                                              16    7355
NP_640339                                                              15    9605
NP_054722                                                              15    9605
NP_001163935                                                           15    9605
NP_001640                                                              15    9605
NP_002586                                                              15    9605
NP_056502                                                              15    9605
NP_001276916                                                           15    9605
XP_005276387                                                           15    9605
XP_005274557                                                           15    9605
XP_005256326                                                           15    9605
XP_006719507                                                           15    9605
XP_006717042                                                           15    9605
XP_005251797                                                           15    9605
XP_005272420                                                           15    9605
XP_005248771                                                           15    9605
XP_005248770                                                           15    9605
XP_005276080                                                           15    9605
>NP_002430
          Length = 1137

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IKGSQTISG 9
           IKGSQ ISG
Sbjct: 483 IKGSQIISG 491
>NP_001006619
          Length = 323

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KGSQTISG 9
           KGSQ +SG
Sbjct: 313 KGSQKVSG 320
>NP_001006618
          Length = 522

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KGSQTISG 9
           KGSQ +SG
Sbjct: 313 KGSQKVSG 320
>NP_001006620
          Length = 475

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KGSQTISG 9
           KGSQ +SG
Sbjct: 313 KGSQKVSG 320
>NP_077022
          Length = 486

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KGSQTISG 9
           KGSQ +SG
Sbjct: 313 KGSQKVSG 320
>NP_001006621
          Length = 330

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KGSQTISG 9
           KGSQ +SG
Sbjct: 121 KGSQKVSG 128
>NP_001006622
          Length = 330

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KGSQTISG 9
           KGSQ +SG
Sbjct: 121 KGSQKVSG 128
>NP_001888
          Length = 2322

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    IKGSQTIS 8
            +KGSQT++
Sbjct: 1584 LKGSQTLT 1591
>NP_114418
          Length = 265

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IKGSQTISG 9
           +KGS+ +SG
Sbjct: 109 LKGSRLVSG 117
>NP_001116451
          Length = 265

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IKGSQTISG 9
           +KGS+ +SG
Sbjct: 109 LKGSRLVSG 117
>NP_001164102
          Length = 577

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IKGSQTISG 9
           +KGS+ +SG
Sbjct: 109 LKGSRLVSG 117
>XP_005273343
          Length = 577

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IKGSQTISG 9
           +KGS+ +SG
Sbjct: 109 LKGSRLVSG 117
>NP_003310
          Length = 26926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IKGSQTISG 9
            +KG+Q I+G
Sbjct: 5376 LKGTQEITG 5384
>NP_597676
          Length = 27051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IKGSQTISG 9
            +KG+Q I+G
Sbjct: 5501 LKGTQEITG 5509
>NP_002200
          Length = 1170

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IKGSQTISG 9
           I+G+Q +SG
Sbjct: 569 IEGTQVLSG 577
>NP_003090
          Length = 522

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GSQTISG 9
           G+QT+SG
Sbjct: 275 GTQTLSG 281
>NP_001243779
          Length = 34350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1     IKGSQTISG 9
             +KG+Q I+G
Sbjct: 12800 LKGTQEITG 12808
>NP_001107852
          Length = 1086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IKGSQTISG 9
           I+G+Q +SG
Sbjct: 486 IEGTQVLSG 494
>NP_001229862
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GSQTISG 9
           G+QT+SG
Sbjct: 275 GTQTLSG 281
>NP_596869
          Length = 33423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1     IKGSQTISG 9
             +KG+Q I+G
Sbjct: 11873 LKGTQEITG 11881
>NP_683758
          Length = 457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GSQTISG 9
           G+QT+SG
Sbjct: 210 GTQTLSG 216
>NP_003881
          Length = 5405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KGSQTIS 8
           +GSQT+S
Sbjct: 912 RGSQTVS 918
>NP_065764
          Length = 892

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  IKGSQTISG 9
          ++G QT+ G
Sbjct: 45 VRGGQTVPG 53
>XP_005260283
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  IKGSQTISG 9
          ++G QT+ G
Sbjct: 59 VRGGQTVPG 67
>XP_006721107
          Length = 1087

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IKGSQTISG 9
           I+G+Q +SG
Sbjct: 486 IEGTQVLSG 494
>XP_005255370
          Length = 1169

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IKGSQTISG 9
           I+G+Q +SG
Sbjct: 569 IEGTQVLSG 577
>XP_005254668
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GSQTISG 9
           G+QT+SG
Sbjct: 117 GTQTLSG 123
>XP_005254667
          Length = 424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GSQTISG 9
           G+QT+SG
Sbjct: 177 GTQTLSG 183
>XP_005246888
          Length = 22663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IKGSQTISG 9
            +KG+Q I+G
Sbjct: 1113 LKGTQEITG 1121
>XP_006712788
          Length = 24022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IKGSQTISG 9
            +KG+Q I+G
Sbjct: 2472 LKGTQEITG 2480
>XP_006712787
          Length = 33978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1     IKGSQTISG 9
             +KG+Q I+G
Sbjct: 12428 LKGTQEITG 12436
>XP_006712786
          Length = 35484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1     IKGSQTISG 9
             +KG+Q I+G
Sbjct: 13934 LKGTQEITG 13942
>NP_001254479
          Length = 35991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1     IKGSQTISG 9
             +KG+Q I+G
Sbjct: 14441 LKGTQEITG 14449
>NP_597681
          Length = 27118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IKGSQTISG 9
            +KG+Q I+G
Sbjct: 5568 LKGTQEITG 5576
>NP_640339
          Length = 516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KGSQTISG 9
           KGS+ +SG
Sbjct: 462 KGSRKVSG 469
>NP_054722
          Length = 1227

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KGSQTI 7
           KGSQT+
Sbjct: 446 KGSQTV 451
>NP_001163935
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IKGSQTI 7
          ++GSQTI
Sbjct: 12 LRGSQTI 18
>NP_001640
          Length = 1616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    KGSQTISG 9
            +GSQ +SG
Sbjct: 1134 RGSQHVSG 1141
>NP_002586
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IKGSQTI 7
          ++GSQTI
Sbjct: 12 LRGSQTI 18
>NP_056502
          Length = 1213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    KGSQTISG 9
            +G+Q++SG
Sbjct: 1140 RGTQSVSG 1147
>NP_001276916
          Length = 1216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    KGSQTISG 9
            +G+Q++SG
Sbjct: 1143 RGTQSVSG 1150
>XP_005276387
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KGSQTISG 9
          +G+Q+ISG
Sbjct: 73 QGNQSISG 80
>XP_005274557
          Length = 1615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    KGSQTISG 9
            +GSQ +SG
Sbjct: 1134 RGSQHVSG 1141
>XP_005256326
          Length = 1192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KGSQTI 7
           KGSQT+
Sbjct: 411 KGSQTV 416
>XP_006719507
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IKGSQTI 7
          ++GSQTI
Sbjct: 12 LRGSQTI 18
>XP_006717042
          Length = 290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KGSQTISG 9
           KGS+ +SG
Sbjct: 236 KGSRKVSG 243
>XP_005251797
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KGSQTISG 9
           KGS+ +SG
Sbjct: 421 KGSRKVSG 428
>XP_005272420
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KGSQTISG 9
          +G+Q+ISG
Sbjct: 73 QGNQSISG 80
>XP_005248771
          Length = 1177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    KGSQTISG 9
            +G+Q++SG
Sbjct: 1140 RGTQSVSG 1147
>XP_005248770
          Length = 1213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    KGSQTISG 9
            +G+Q++SG
Sbjct: 1140 RGTQSVSG 1147
>XP_005276080
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KGSQTISG 9
          +G+Q+ISG
Sbjct: 73 QGNQSISG 80
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.125    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 498,151
Number of extensions: 748
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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