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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              17    2528
XP_006716142                                                           17    2528
XP_005250088                                                           17    2528
XP_006716141                                                           17    2528
XP_005250085                                                           17    2528
XP_006716140                                                           17    2528
XP_005250084                                                           17    2528
XP_005250083                                                           17    2528
XP_005250082                                                           17    2528
XP_005263028                                                           16    5631
NP_003855                                                              16    5631
NP_001156372                                                           16    7355
NP_001273330                                                           16    7355
NP_001124535                                                           16    7355
NP_001124534                                                           16    7355
NP_078908                                                              16    7355
NP_660359                                                              16    7355
NP_001273387                                                           15    9605
NP_003461                                                              15    9605
NP_001273386                                                           15    9605

>NP_733751
          Length = 4911

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IISFSRRIQ 9
            IIS SRRIQ
Sbjct: 4869 IISSSRRIQ 4877



>XP_006716142
          Length = 4927

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IISFSRRIQ 9
            IIS SRRIQ
Sbjct: 4885 IISSSRRIQ 4893



>XP_005250088
          Length = 4928

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IISFSRRIQ 9
            IIS SRRIQ
Sbjct: 4886 IISSSRRIQ 4894



>XP_006716141
          Length = 4959

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IISFSRRIQ 9
            IIS SRRIQ
Sbjct: 4917 IISSSRRIQ 4925



>XP_005250085
          Length = 4979

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IISFSRRIQ 9
            IIS SRRIQ
Sbjct: 4937 IISSSRRIQ 4945



>XP_006716140
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IISFSRRIQ 9
            IIS SRRIQ
Sbjct: 4940 IISSSRRIQ 4948



>XP_005250084
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IISFSRRIQ 9
            IIS SRRIQ
Sbjct: 4940 IISSSRRIQ 4948



>XP_005250083
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IISFSRRIQ 9
            IIS SRRIQ
Sbjct: 4940 IISSSRRIQ 4948



>XP_005250082
          Length = 4983

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IISFSRRIQ 9
            IIS SRRIQ
Sbjct: 4941 IISSSRRIQ 4949



>XP_005263028
          Length = 152

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IISFSRR 7
           +ISFSRR
Sbjct: 129 LISFSRR 135



>NP_003855
          Length = 220

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SFSRRIQ 9
          SFS+RIQ
Sbjct: 84 SFSKRIQ 90



>NP_001156372
          Length = 202

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IISFSRRI 8
           IIS+ RRI
Sbjct: 162 IISYKRRI 169



>NP_001273330
          Length = 211

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IISFSRRI 8
           IIS+ RRI
Sbjct: 162 IISYKRRI 169



>NP_001124535
          Length = 137

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SFSRRIQ 9
          SFS+R+Q
Sbjct: 46 SFSKRVQ 52



>NP_001124534
          Length = 142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SFSRRIQ 9
          SFS+R+Q
Sbjct: 46 SFSKRVQ 52



>NP_078908
          Length = 183

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SFSRRIQ 9
          SFS+R+Q
Sbjct: 46 SFSKRVQ 52



>NP_660359
          Length = 229

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IISFSRRI 8
           IIS+ RRI
Sbjct: 162 IISYKRRI 169



>NP_001273387
          Length = 1003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  SFSRRI 8
          SFSRRI
Sbjct: 50 SFSRRI 55



>NP_003461
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRRI 8
           SFSRRI
Sbjct: 149 SFSRRI 154



>NP_001273386
          Length = 1086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SFSRRI 8
           SFSRRI
Sbjct: 133 SFSRRI 138



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.347    0.151    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 383,319
Number of extensions: 332
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)