Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1135
NP_001075109                                                           18    1135
NP_000375                                                              17    3301
XP_006716433                                                           17    4312
XP_005273633                                                           17    4312
XP_006716432                                                           17    4312
XP_005273632                                                           17    4312
NP_001276915                                                           16    7355
NP_060328                                                              16    7355
NP_073616                                                              16    7355
NP_001153508                                                           16    7355
XP_006720657                                                           16    7355
XP_005254579                                                           16    7355
XP_005254578                                                           16    7355
XP_006720656                                                           16    7355
XP_006717299                                                           16    7355
XP_005272265                                                           16    7355
XP_005272264                                                           16    7355
XP_005272263                                                           16    7355
XP_006716378                                                           16    7355
XP_006711056                                                           16    7355
NP_001185498                                                           16    7355
NP_001685                                                              16    7355
NP_001153509                                                           16    7355
NP_055993                                                              16    7355
NP_115266                                                              16    7355
NP_619526                                                              16    7355
NP_061745                                                              16    7355
NP_008914                                                              16    7355
NP_000043                                                              15    9605
NP_001269153                                                           15    9605
NP_001275915                                                           15    9605
NP_001275914                                                           15    9605
NP_009099                                                              15    9605
XP_005256997                                                           15    9605
XP_005256995                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IIANALNSE 9
            IIANAL+SE
Sbjct: 3336 IIANALSSE 3344
>NP_001075109
          Length = 4097

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IIANALNSE 9
            IIANAL+SE
Sbjct: 3336 IIANALSSE 3344
>NP_000375
          Length = 4563

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   IANALNSE 9
           IAN LNSE
Sbjct: 597 IANILNSE 604
>XP_006716433
          Length = 3069

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IANALNSE 9
           + NALNSE
Sbjct: 221 LCNALNSE 228
>XP_005273633
          Length = 3069

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IANALNSE 9
           + NALNSE
Sbjct: 221 LCNALNSE 228
>XP_006716432
          Length = 3172

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IANALNSE 9
           + NALNSE
Sbjct: 324 LCNALNSE 331
>XP_005273632
          Length = 3172

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IANALNSE 9
           + NALNSE
Sbjct: 324 LCNALNSE 331
>NP_001276915
          Length = 200

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   IANALNSE 9
           IAN LN E
Sbjct: 167 IANTLNEE 174
>NP_060328
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   IANALNSE 9
           IAN LN E
Sbjct: 268 IANTLNEE 275
>NP_073616
          Length = 851

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIANALNSE 9
           +I NA+N+E
Sbjct: 404 VILNAVNAE 412
>NP_001153508
          Length = 749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIANALNSE 9
           ++AN LN+E
Sbjct: 485 MVANILNNE 493
>XP_006720657
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   IANALNSE 9
           IAN LN E
Sbjct: 268 IANTLNEE 275
>XP_005254579
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   IANALNSE 9
           IAN LN E
Sbjct: 268 IANTLNEE 275
>XP_005254578
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   IANALNSE 9
           IAN LN E
Sbjct: 268 IANTLNEE 275
>XP_006720656
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   IANALNSE 9
           IAN LN E
Sbjct: 268 IANTLNEE 275
>XP_006717299
          Length = 691

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIANALNSE 9
           +I NA+N+E
Sbjct: 404 VILNAVNAE 412
>XP_005272265
          Length = 722

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIANALNSE 9
           +I NA+N+E
Sbjct: 275 VILNAVNAE 283
>XP_005272264
          Length = 755

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIANALNSE 9
           +I NA+N+E
Sbjct: 308 VILNAVNAE 316
>XP_005272263
          Length = 778

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIANALNSE 9
           +I NA+N+E
Sbjct: 404 VILNAVNAE 412
>XP_006716378
          Length = 749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIANALNSE 9
           ++AN LN+E
Sbjct: 485 MVANILNNE 493
>XP_006711056
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IIANALN 7
           IIANA+N
Sbjct: 357 IIANAVN 363
>NP_001185498
          Length = 155

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  IIANALNSE 9
          +IAN+LN +
Sbjct: 75 LIANSLNDD 83
>NP_001685
          Length = 155

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  IIANALNSE 9
          +IAN+LN +
Sbjct: 75 LIANSLNDD 83
>NP_001153509
          Length = 734

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIANALNSE 9
           ++AN LN+E
Sbjct: 470 MVANILNNE 478
>NP_055993
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIANALNSE 9
           ++AN LN+E
Sbjct: 463 MVANILNNE 471
>NP_115266
          Length = 810

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IIANALNSE 9
           +IA ALN E
Sbjct: 401 VIAGALNRE 409
>NP_619526
          Length = 585

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIANALNSE 9
           +I NA+N+E
Sbjct: 404 VILNAVNAE 412
>NP_061745
          Length = 927

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IIANALNSE 9
           +IA ALN E
Sbjct: 401 VIAGALNRE 409
>NP_008914
          Length = 390

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IIANALN 7
           IIANA+N
Sbjct: 358 IIANAVN 364
>NP_000043
          Length = 1500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    IANALNSE 9
            I+NALN++
Sbjct: 1169 ISNALNAQ 1176
>NP_001269153
          Length = 1422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    IANALNSE 9
            I+NALN++
Sbjct: 1091 ISNALNAQ 1098
>NP_001275915
          Length = 1616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    ANALNS 8
            ANALNS
Sbjct: 1261 ANALNS 1266
>NP_001275914
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    ANALNS 8
            ANALNS
Sbjct: 1266 ANALNS 1271
>NP_009099
          Length = 1581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    ANALNS 8
            ANALNS
Sbjct: 1226 ANALNS 1231
>XP_005256997
          Length = 1560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    ANALNS 8
            ANALNS
Sbjct: 1205 ANALNS 1210
>XP_005256995
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    ANALNS 8
            ANALNS
Sbjct: 1266 ANALNS 1271
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.121    0.282 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 285,942
Number of extensions: 346
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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