Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1135
NP_001075109                                                           18    1135
NP_443728                                                              15    9605
NP_722541                                                              15    9605
NP_001092140                                                           15    9605
NP_001092141                                                           15    9605
XP_006718478                                                           15    9605
XP_006718477                                                           15    9605
XP_006718476                                                           15    9605
XP_006718475                                                           15    9605
XP_005273764                                                           15    9605
XP_006718474                                                           15    9605
XP_006713550                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IIAKQLFS 8
            IIAKQLFS
Sbjct: 2803 IIAKQLFS 2810
>NP_001075109
          Length = 4097

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    IIAKQLFS 8
            IIAKQLFS
Sbjct: 2803 IIAKQLFS 2810
>NP_443728
          Length = 2145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IIAKQLFSI 9
            +IA+Q FS+
Sbjct: 1091 LIARQCFSL 1099
>NP_722541
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQLFSI 9
           KQLFSI
Sbjct: 335 KQLFSI 340
>NP_001092140
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQLFSI 9
           KQLFSI
Sbjct: 335 KQLFSI 340
>NP_001092141
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQLFSI 9
           KQLFSI
Sbjct: 335 KQLFSI 340
>XP_006718478
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQLFSI 9
           KQLFSI
Sbjct: 190 KQLFSI 195
>XP_006718477
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQLFSI 9
           KQLFSI
Sbjct: 190 KQLFSI 195
>XP_006718476
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQLFSI 9
           KQLFSI
Sbjct: 335 KQLFSI 340
>XP_006718475
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQLFSI 9
           KQLFSI
Sbjct: 335 KQLFSI 340
>XP_005273764
          Length = 668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQLFSI 9
           KQLFSI
Sbjct: 394 KQLFSI 399
>XP_006718474
          Length = 897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KQLFSI 9
           KQLFSI
Sbjct: 623 KQLFSI 628
>XP_006713550
          Length = 2180

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IIAKQLFSI 9
            +IA+Q FS+
Sbjct: 1126 LIARQCFSL 1134
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.152    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 325,074
Number of extensions: 321
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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