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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              18    1135
XP_006713721                                                           18    1135
NP_003045                                                              18    1482
NP_062818                                                              17    4312
NP_001136277                                                           16    5631
NP_005620                                                              16    5631
NP_722580                                                              16    5631
XP_006710057                                                           16    5631
NP_898871                                                              16    7355
NP_000047                                                              16    7355
NP_001138762                                                           16    7355
NP_938018                                                              16    7355
NP_001093591                                                           16    7355
NP_001093590                                                           16    7355
XP_006715605                                                           16    7355
XP_005248813                                                           16    7355
NP_001032401                                                           16    7355
NP_055508                                                              16    7355
NP_004516                                                              15    9605
NP_116191                                                              15    9605
NP_001264044                                                           15    9605
NP_009162                                                              15    9605
NP_003033                                                              15    9605
NP_001010898                                                           15    9605
XP_005254779                                                           15    9605
XP_006713369                                                           15    9605
XP_005265468                                                           15    9605
XP_005265467                                                           15    9605
XP_006712591                                                           15    9605
XP_006712590                                                           15    9605
XP_006712589                                                           15    9605
XP_006710706                                                           15    9605
NP_001185498                                                           15    9605
NP_001685                                                              15    9605

>NP_006209
          Length = 1068

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IGFAIGMLV 9
           IGFAIGM V
Sbjct: 117 IGFAIGMPV 125



>XP_006713721
          Length = 1068

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IGFAIGMLV 9
           IGFAIGM V
Sbjct: 117 IGFAIGMPV 125



>NP_003045
          Length = 514

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IGFAIGML 8
           +GFAIGM+
Sbjct: 391 VGFAIGMV 398



>NP_062818
          Length = 702

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IGFAIGML 8
           IGFA+G L
Sbjct: 222 IGFALGSL 229



>NP_001136277
          Length = 625

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  IGFAIGM 7
          +GFA+G+
Sbjct: 66 VGFAVGL 72



>NP_005620
          Length = 635

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  IGFAIGM 7
          +GFA+G+
Sbjct: 66 VGFAVGL 72



>NP_722580
          Length = 695

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   IGFAIG 6
           IGFAIG
Sbjct: 517 IGFAIG 522



>XP_006710057
          Length = 99

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  IGFAIGM 7
          +GFA+G+
Sbjct: 8  VGFAVGL 14



>NP_898871
          Length = 392

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGFAIGMLV 9
           +GF IG+ V
Sbjct: 130 VGFGIGIAV 138



>NP_000047
          Length = 392

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGFAIGMLV 9
           +GF IG+ V
Sbjct: 130 VGFGIGIAV 138



>NP_001138762
          Length = 501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGFAIGMLV 9
           I +AIGM +
Sbjct: 96  IAYAIGMFI 104



>NP_938018
          Length = 505

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGFAIGMLV 9
           I +AIGM +
Sbjct: 96  IAYAIGMFI 104



>NP_001093591
          Length = 765

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IGFAIGML 8
           +GFA+G L
Sbjct: 438 LGFALGSL 445



>NP_001093590
          Length = 811

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IGFAIGML 8
           +GFA+G L
Sbjct: 484 LGFALGSL 491



>XP_006715605
          Length = 322

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  IGFAIGMLV 9
          +GF IG+ V
Sbjct: 60 VGFGIGIAV 68



>XP_005248813
          Length = 360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGFAIGMLV 9
           +GF IG+ V
Sbjct: 130 VGFGIGIAV 138



>NP_001032401
          Length = 736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IGFAIGML 8
           +GFA+G L
Sbjct: 409 LGFALGSL 416



>NP_055508
          Length = 883

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IGFAIGML 8
           +GFA+G L
Sbjct: 556 LGFALGSL 563



>NP_004516
          Length = 4655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    IGFAIGMLV 9
            +GF IG+ V
Sbjct: 1870 VGFPIGITV 1878



>NP_116191
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IGFAIGMLV 9
           + +AIGM++
Sbjct: 442 VAYAIGMIL 450



>NP_001264044
          Length = 4516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IGFAIGMLV 9
            +G A+GM+V
Sbjct: 1906 LGRALGMMV 1914



>NP_009162
          Length = 642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  IGFAIGM 7
          IG+A+G+
Sbjct: 50 IGYAVGL 56



>NP_003033
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  IGFAIGM 7
          +G+AIG+
Sbjct: 58 VGYAIGL 64



>NP_001010898
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  IGFAIGM 7
          IGF++G+
Sbjct: 74 IGFSVGL 80



>XP_005254779
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IGFAIGMLV 9
           + +AIGM++
Sbjct: 460 VAYAIGMIL 468



>XP_006713369
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  IGFAIGM 7
          +G+AIG+
Sbjct: 58 VGYAIGL 64



>XP_005265468
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  IGFAIGM 7
          +G+AIG+
Sbjct: 58 VGYAIGL 64



>XP_005265467
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  IGFAIGM 7
          +G+AIG+
Sbjct: 58 VGYAIGL 64



>XP_006712591
          Length = 3308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    IGFAIGMLV 9
            +GF IG+ V
Sbjct: 1875 VGFPIGITV 1883



>XP_006712590
          Length = 3897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    IGFAIGMLV 9
            +GF IG+ V
Sbjct: 1112 VGFPIGITV 1120



>XP_006712589
          Length = 4660

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    IGFAIGMLV 9
            +GF IG+ V
Sbjct: 1875 VGFPIGITV 1883



>XP_006710706
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  IGFAIGM 7
          IGF++G+
Sbjct: 74 IGFSVGL 80



>NP_001185498
          Length = 155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GFAIGML 8
           GFAIG++
Sbjct: 107 GFAIGIV 113



>NP_001685
          Length = 155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GFAIGML 8
           GFAIG++
Sbjct: 107 GFAIGIV 113



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.166    0.483 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 353,392
Number of extensions: 548
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)