Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     299
NP_001075109                                                           20     299
NP_006437                                                              18    1135
NP_062818                                                              17    2528
NP_001009562                                                           17    2528
NP_000042                                                              17    4312
NP_056163                                                              17    4312
XP_006718908                                                           17    4312
XP_005271621                                                           17    4312
XP_006718907                                                           17    4312
XP_006718906                                                           17    4312
XP_005271619                                                           17    4312
XP_005271618                                                           17    4312
XP_005274106                                                           17    4312
XP_005274105                                                           17    4312
XP_005263969                                                           17    4312
XP_005263968                                                           17    4312
XP_005263966                                                           17    4312
NP_056969                                                              17    4312
NP_067585                                                              16    5631
NP_004318                                                              16    5631
NP_116584                                                              16    7355
NP_004750                                                              16    7355
NP_001810                                                              16    7355
NP_001264044                                                           16    7355
NP_001517                                                              16    7355
NP_004626                                                              16    7355
NP_001230854                                                           16    7355
NP_055898                                                              16    7355
NP_057591                                                              16    7355
NP_001230855                                                           16    7355
NP_112557                                                              16    7355
NP_004715                                                              16    7355
NP_003709                                                              16    7355
XP_005259653                                                           16    7355
XP_005257515                                                           16    7355
XP_005257513                                                           16    7355
XP_006719006                                                           16    7355
XP_005272378                                                           16    7355
XP_006715137                                                           16    7355
XP_005273410                                                           16    7355
NP_001135977                                                           16    7355
NP_038460                                                              16    7355
NP_071452                                                              16    7355
NP_002723                                                              16    7355
NP_064555                                                              16    7355
NP_060835                                                              15    9605
NP_699192                                                              15    9605
NP_001118230                                                           15    9605
NP_817089                                                              15    9605
NP_055449                                                              15    9605
NP_001018011                                                           15    9605
NP_005997                                                              15    9605
NP_001073981                                                           15    9605
XP_006721512                                                           15    9605
XP_006721511                                                           15    9605
XP_005271715                                                           15    9605
XP_006718962                                                           15    9605
XP_005270227                                                           15    9605
XP_005270226                                                           15    9605
XP_005270225                                                           15    9605
XP_005270224                                                           15    9605
XP_005270223                                                           15    9605
XP_006714514                                                           15    9605
XP_006714513                                                           15    9605
>NP_008835
          Length = 4128

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IGELFSKLY 9
           IGELFSK Y
Sbjct: 149 IGELFSKFY 157
>NP_001075109
          Length = 4097

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IGELFSKLY 9
           IGELFSK Y
Sbjct: 149 IGELFSKFY 157
>NP_006437
          Length = 691

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IGELFSKLY 9
           +G LFSK+Y
Sbjct: 226 LGSLFSKMY 234
>NP_062818
          Length = 702

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IGELFSKLY 9
           +G LF+K+Y
Sbjct: 226 LGSLFAKMY 234
>NP_001009562
          Length = 640

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IGELFSKLY 9
           +G LF+K+Y
Sbjct: 179 LGSLFAKMY 187
>NP_000042
          Length = 3056

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    IGELFSK 7
            IGELFS+
Sbjct: 2185 IGELFSR 2191
>NP_056163
          Length = 1883

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GELFSKL 8
            GE+FSKL
Sbjct: 1735 GEVFSKL 1741
>XP_006718908
          Length = 1708

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IGELFSK 7
           IGELFS+
Sbjct: 837 IGELFSR 843
>XP_005271621
          Length = 2708

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    IGELFSK 7
            IGELFS+
Sbjct: 1837 IGELFSR 1843
>XP_006718907
          Length = 3050

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    IGELFSK 7
            IGELFS+
Sbjct: 2179 IGELFSR 2185
>XP_006718906
          Length = 3056

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    IGELFSK 7
            IGELFS+
Sbjct: 2185 IGELFSR 2191
>XP_005271619
          Length = 3056

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    IGELFSK 7
            IGELFS+
Sbjct: 2185 IGELFSR 2191
>XP_005271618
          Length = 3056

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    IGELFSK 7
            IGELFS+
Sbjct: 2185 IGELFSR 2191
>XP_005274106
          Length = 1685

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    IGELFSKL 8
            + ELFSKL
Sbjct: 1400 VNELFSKL 1407
>XP_005274105
          Length = 1686

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    IGELFSKL 8
            + ELFSKL
Sbjct: 1400 VNELFSKL 1407
>XP_005263969
          Length = 1844

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GELFSKL 8
            GE+FSKL
Sbjct: 1696 GEVFSKL 1702
>XP_005263968
          Length = 1861

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GELFSKL 8
            GE+FSKL
Sbjct: 1713 GEVFSKL 1719
>XP_005263966
          Length = 1900

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GELFSKL 8
            GE+FSKL
Sbjct: 1752 GEVFSKL 1758
>NP_056969
          Length = 1555

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    IGELFSKL 8
            + ELFSKL
Sbjct: 1269 VNELFSKL 1276
>NP_067585
          Length = 1227

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IGELFSKL 8
           +G+LF KL
Sbjct: 577 VGDLFQKL 584
>NP_004318
          Length = 1271

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IGELFSKL 8
           +G+LF KL
Sbjct: 577 VGDLFQKL 584
>NP_116584
          Length = 400

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GELFSKL 8
           GELFS++
Sbjct: 144 GELFSRI 150
>NP_004750
          Length = 370

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GELFSKL 8
           GELFS++
Sbjct: 144 GELFSRI 150
>NP_001810
          Length = 677

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGELFSKLY 9
           +GELF+  Y
Sbjct: 518 LGELFNPYY 526
>NP_001264044
          Length = 4516

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GELFSKLY 9
           G+LF+K Y
Sbjct: 867 GDLFTKKY 874
>NP_001517
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   GELFSKLY 9
           GE++ K+Y
Sbjct: 216 GEIYPKMY 223
>NP_004626
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GELFSKL 8
           GELFS++
Sbjct: 124 GELFSRI 130
>NP_001230854
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GELFSKL 8
           GELFS++
Sbjct: 124 GELFSRI 130
>NP_055898
          Length = 1481

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IGELFSK 7
           +GELF+K
Sbjct: 926 LGELFTK 932
>NP_057591
          Length = 1490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IGELFSK 7
           +GELF+K
Sbjct: 926 LGELFTK 932
>NP_001230855
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GELFSKL 8
           GELFS++
Sbjct: 124 GELFSRI 130
>NP_112557
          Length = 1452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IGELFSK 7
           +GELF+K
Sbjct: 904 LGELFTK 910
>NP_004715
          Length = 779

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IGELFSKL 8
           +GEL SKL
Sbjct: 234 LGELHSKL 241
>NP_003709
          Length = 1512

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IGELFSK 7
           +GELF+K
Sbjct: 904 LGELFTK 910
>XP_005259653
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGELFSKLY 9
           +  LFS+LY
Sbjct: 251 LARLFSRLY 259
>XP_005257515
          Length = 1256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IGELFSK 7
           +GELF+K
Sbjct: 926 LGELFTK 932
>XP_005257513
          Length = 1489

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IGELFSK 7
           +GELF+K
Sbjct: 925 LGELFTK 931
>XP_006719006
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IGELFSKL 8
           +GEL SKL
Sbjct: 234 LGELHSKL 241
>XP_005272378
          Length = 1377

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IGELFSK 7
           +G+LFSK
Sbjct: 317 LGDLFSK 323
>XP_006715137
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   GELFSKLY 9
           GE++ K+Y
Sbjct: 216 GEIYPKMY 223
>XP_005273410
          Length = 371

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GELFSKL 8
           GELFS++
Sbjct: 144 GELFSRI 150
>NP_001135977
          Length = 646

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   ELFSKLY 9
           E+F KLY
Sbjct: 266 EMFDKLY 272
>NP_038460
          Length = 1378

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IGELFSK 7
           +G+LFSK
Sbjct: 317 LGDLFSK 323
>NP_071452
          Length = 646

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   ELFSKLY 9
           E+F KLY
Sbjct: 266 EMFDKLY 272
>NP_002723
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GELFSKL 8
           GE+FS+L
Sbjct: 127 GEMFSRL 133
>NP_064555
          Length = 563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGELFSKLY 9
           +  LFS+LY
Sbjct: 251 LARLFSRLY 259
>NP_060835
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGELFSKLY 9
           +GE F K+Y
Sbjct: 477 LGEEFRKVY 485
>NP_699192
          Length = 436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GELFSKL 8
           GELF K+
Sbjct: 156 GELFGKI 162
>NP_001118230
          Length = 549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IGELFSK 7
           IG+LF+K
Sbjct: 218 IGDLFTK 224
>NP_817089
          Length = 832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  IGELFSKL 8
          IGELF+ +
Sbjct: 75 IGELFNNM 82
>NP_055449
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGELFSKLY 9
           IG++F K Y
Sbjct: 328 IGDVFQKPY 336
>NP_001018011
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ELFSKL 8
           ELFSKL
Sbjct: 383 ELFSKL 388
>NP_005997
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ELFSKL 8
           ELFSKL
Sbjct: 383 ELFSKL 388
>NP_001073981
          Length = 974

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ELFSKL 8
           ELFSKL
Sbjct: 880 ELFSKL 885
>XP_006721512
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IGELFSK 7
          IG+LF+K
Sbjct: 48 IGDLFTK 54
>XP_006721511
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IGELFSK 7
          IG+LF+K
Sbjct: 67 IGDLFTK 73
>XP_005271715
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ELFSKL 8
           ELFSKL
Sbjct: 383 ELFSKL 388
>XP_006718962
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ELFSKL 8
           ELFSKL
Sbjct: 383 ELFSKL 388
>XP_005270227
          Length = 909

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ELFSKL 8
           ELFSKL
Sbjct: 815 ELFSKL 820
>XP_005270226
          Length = 927

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ELFSKL 8
           ELFSKL
Sbjct: 833 ELFSKL 838
>XP_005270225
          Length = 949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ELFSKL 8
           ELFSKL
Sbjct: 880 ELFSKL 885
>XP_005270224
          Length = 949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ELFSKL 8
           ELFSKL
Sbjct: 880 ELFSKL 885
>XP_005270223
          Length = 973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ELFSKL 8
           ELFSKL
Sbjct: 879 ELFSKL 884
>XP_006714514
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  GELFSKL 8
          GELF K+
Sbjct: 32 GELFGKI 38
>XP_006714513
          Length = 436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GELFSKL 8
           GELF K+
Sbjct: 156 GELFGKI 162
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.153    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 471,130
Number of extensions: 892
Number of successful extensions: 65
Number of sequences better than 10000.0: 65
Number of HSP's gapped: 65
Number of HSP's successfully gapped: 65
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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