Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              19     665
XP_006713721                                                           19     665
NP_003801                                                              17    3301
NP_002477                                                              16    5631
NP_036529                                                              16    5631
XP_005266764                                                           16    5631
XP_005266762                                                           16    5631
XP_005252054                                                           16    5631
NP_060790                                                              16    7355
XP_006718697                                                           16    7355
XP_006718695                                                           16    7355
XP_006718693                                                           16    7355
XP_006718692                                                           16    7355
NP_001124388                                                           15    9605
NP_004603                                                              15    9605
NP_000341                                                              15    9605
NP_001271169                                                           15    9605
NP_001271170                                                           15    9605
NP_061872                                                              15    9605
XP_005269963                                                           15    9605
XP_005269962                                                           15    9605
XP_005252207                                                           15    9605
XP_006717224                                                           15    9605
XP_005266150                                                           15    9605
XP_006717221                                                           15    9605
XP_006717220                                                           15    9605
XP_006717219                                                           15    9605
XP_006717216                                                           15    9605
XP_006717215                                                           15    9605
XP_006717214                                                           15    9605
>NP_006209
          Length = 1068

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  IFVSVTEEA 9
          IFVSVT+EA
Sbjct: 69 IFVSVTQEA 77
>XP_006713721
          Length = 1068

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  IFVSVTEEA 9
          IFVSVT+EA
Sbjct: 69 IFVSVTQEA 77
>NP_003801
          Length = 281

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   IFVSVTEE 8
           IFVSVT E
Sbjct: 256 IFVSVTNE 263
>NP_002477
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FVSVTEE 8
           FVSVT+E
Sbjct: 152 FVSVTQE 158
>NP_036529
          Length = 391

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FVSVTEE 8
           FV+VTEE
Sbjct: 335 FVAVTEE 341
>XP_005266764
          Length = 255

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FVSVTEE 8
           FV+VTEE
Sbjct: 199 FVAVTEE 205
>XP_005266762
          Length = 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FVSVTEE 8
           FV+VTEE
Sbjct: 344 FVAVTEE 350
>XP_005252054
          Length = 723

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  FVSVTEE 8
          FVSVT+E
Sbjct: 85 FVSVTQE 91
>NP_060790
          Length = 327

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IFVSVTEE 8
           IFV V+EE
Sbjct: 233 IFVDVSEE 240
>XP_006718697
          Length = 199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IFVSVTEE 8
           IFV V+EE
Sbjct: 105 IFVDVSEE 112
>XP_006718695
          Length = 220

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IFVSVTEE 8
           IFV V+EE
Sbjct: 126 IFVDVSEE 133
>XP_006718693
          Length = 246

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IFVSVTEE 8
           IFV V+EE
Sbjct: 152 IFVDVSEE 159
>XP_006718692
          Length = 291

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IFVSVTEE 8
           IFV V+EE
Sbjct: 197 IFVDVSEE 204
>NP_001124388
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FVSVTE 7
          FVSVTE
Sbjct: 55 FVSVTE 60
>NP_004603
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FVSVTE 7
          FVSVTE
Sbjct: 55 FVSVTE 60
>NP_000341
          Length = 2273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IFVSVTEEA 9
            IF+ VTE++
Sbjct: 1272 IFLKVTEDS 1280
>NP_001271169
          Length = 1047

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IFVSVTEE 8
           +FVS TEE
Sbjct: 130 MFVSSTEE 137
>NP_001271170
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IFVSVTEE 8
           +FVS TEE
Sbjct: 130 MFVSSTEE 137
>NP_061872
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IFVSVTEE 8
           +FVS TEE
Sbjct: 130 MFVSSTEE 137
>XP_005269963
          Length = 837

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IFVSVTEE 8
           +FVS TEE
Sbjct: 130 MFVSSTEE 137
>XP_005269962
          Length = 850

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IFVSVTEE 8
           +FVS TEE
Sbjct: 130 MFVSSTEE 137
>XP_005252207
          Length = 507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FVSVTE 7
          FVSVTE
Sbjct: 55 FVSVTE 60
>XP_006717224
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FVSVTEEA 9
          FVS+ EEA
Sbjct: 90 FVSLREEA 97
>XP_005266150
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FVSVTEEA 9
          FVS+ EEA
Sbjct: 90 FVSLREEA 97
>XP_006717221
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FVSVTEEA 9
          FVS+ EEA
Sbjct: 90 FVSLREEA 97
>XP_006717220
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FVSVTEEA 9
          FVS+ EEA
Sbjct: 90 FVSLREEA 97
>XP_006717219
          Length = 856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FVSVTEEA 9
          FVS+ EEA
Sbjct: 90 FVSLREEA 97
>XP_006717216
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FVSVTEEA 9
          FVS+ EEA
Sbjct: 90 FVSLREEA 97
>XP_006717215
          Length = 928

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FVSVTEEA 9
          FVS+ EEA
Sbjct: 90 FVSLREEA 97
>XP_006717214
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FVSVTEEA 9
          FVS+ EEA
Sbjct: 90 FVSLREEA 97
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.127    0.301 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 405,681
Number of extensions: 486
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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