Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              20     390
NP_001268421                                                           17    2528
NP_000170                                                              17    2528
NP_001268422                                                           17    2528
NP_001268423                                                           17    2528
XP_005264328                                                           17    2528
NP_004760                                                              17    4312
NP_064717                                                              17    4312
NP_064718                                                              17    4312
NP_001275896                                                           15    9605
NP_001275895                                                           15    9605
NP_001275894                                                           15    9605
NP_077817                                                              15    9605
NP_001275893                                                           15    9605
XP_005247869                                                           15    9605
XP_006713830                                                           15    9605
XP_005247867                                                           15    9605
XP_005247866                                                           15    9605
XP_005247863                                                           15    9605
XP_005247862                                                           15    9605
XP_005247861                                                           15    9605
XP_005247860                                                           15    9605
XP_005247859                                                           15    9605
NP_004585                                                              15    9605
>NP_002430
          Length = 1137

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IFTRMGAAN 9
           IFTRMGAA+
Sbjct: 935 IFTRMGAAD 943
>NP_001268421
          Length = 1230

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    IFTRMGAAN 9
            +FTR+GA++
Sbjct: 1043 VFTRLGASD 1051
>NP_000170
          Length = 1360

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    IFTRMGAAN 9
            +FTR+GA++
Sbjct: 1173 VFTRLGASD 1181
>NP_001268422
          Length = 1058

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   IFTRMGAAN 9
           +FTR+GA++
Sbjct: 871 VFTRLGASD 879
>NP_001268423
          Length = 1058

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   IFTRMGAAN 9
           +FTR+GA++
Sbjct: 871 VFTRLGASD 879
>XP_005264328
          Length = 1261

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    IFTRMGAAN 9
            +FTR+GA++
Sbjct: 1074 VFTRLGASD 1082
>NP_004760
          Length = 531

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   IFTRMG 6
           IFTRMG
Sbjct: 179 IFTRMG 184
>NP_064717
          Length = 549

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   IFTRMG 6
           IFTRMG
Sbjct: 179 IFTRMG 184
>NP_064718
          Length = 543

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   IFTRMG 6
           IFTRMG
Sbjct: 179 IFTRMG 184
>NP_001275896
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 340 VYTRLGEMN 348
>NP_001275895
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 492 VYTRLGEMN 500
>NP_001275894
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 472 VYTRLGEMN 480
>NP_077817
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 492 VYTRLGEMN 500
>NP_001275893
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 474 VYTRLGEMN 482
>XP_005247869
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 233 VYTRLGEMN 241
>XP_006713830
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 316 VYTRLGEMN 324
>XP_005247867
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 340 VYTRLGEMN 348
>XP_005247866
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 492 VYTRLGEMN 500
>XP_005247863
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 492 VYTRLGEMN 500
>XP_005247862
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 492 VYTRLGEMN 500
>XP_005247861
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 472 VYTRLGEMN 480
>XP_005247860
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 492 VYTRLGEMN 500
>XP_005247859
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   IFTRMGAAN 9
           ++TR+G  N
Sbjct: 492 VYTRLGEMN 500
>NP_004585
          Length = 896

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FTRMGAAN 9
           F  MG+AN
Sbjct: 227 FVEMGSAN 234
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.136    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 306,042
Number of extensions: 311
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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