Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443117                                                              18    1935
XP_005268673                                                           18    1935
NP_001683                                                              17    2528
XP_005264425                                                           17    2528
NP_004037                                                              17    3301
NP_001001937                                                           17    3301
NP_001244264                                                           17    3301
NP_001001935                                                           17    3301
NP_001244263                                                           17    3301
NP_004688                                                              17    4312
NP_001231855                                                           17    4312
NP_063946                                                              16    5631
NP_001137446                                                           16    5631
NP_001684                                                              16    5631
NP_060352                                                              16    7355
NP_001257880                                                           16    7355
NP_001257879                                                           16    7355
NP_003830                                                              16    7355
NP_001265197                                                           16    7355
NP_758424                                                              16    7355
NP_061142                                                              16    7355
NP_001265354                                                           16    7355
NP_443124                                                              16    7355
NP_001177951                                                           15    9605
NP_006556                                                              15    9605
NP_114409                                                              15    9605
NP_995322                                                              15    9605
NP_001255979                                                           15    9605
NP_001255983                                                           15    9605
NP_001255971                                                           15    9605
NP_001255970                                                           15    9605
NP_001255978                                                           15    9605
NP_001255972                                                           15    9605
NP_001255981                                                           15    9605
NP_542185                                                              15    9605
NP_001255976                                                           15    9605
NP_001255975                                                           15    9605
NP_001255974                                                           15    9605
NP_001255973                                                           15    9605
NP_001255969                                                           15    9605
NP_001255984                                                           15    9605
NP_001255977                                                           15    9605
NP_001255980                                                           15    9605
NP_006412                                                              15    9605
NP_001269869                                                           15    9605
NP_001034934                                                           15    9605
NP_001032394                                                           15    9605
NP_001010861                                                           15    9605
NP_060223                                                              15    9605
XP_005255832                                                           15    9605
XP_005254365                                                           15    9605
XP_006716481                                                           15    9605
XP_005251193                                                           15    9605
XP_005251192                                                           15    9605
XP_005251191                                                           15    9605
XP_005246710                                                           15    9605
XP_005246372                                                           15    9605
NP_061921                                                              15    9605
NP_997182                                                              15    9605
>NP_443117
          Length = 648

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IFITSGQIF 9
           +FIT GQ F
Sbjct: 214 LFITGGQFF 222
>XP_005268673
          Length = 555

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IFITSGQIF 9
           +FIT GQ F
Sbjct: 214 LFITGGQFF 222
>NP_001683
          Length = 513

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FITSGQIF 9
           FIT GQI+
Sbjct: 365 FITEGQIY 372
>XP_005264425
          Length = 471

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FITSGQIF 9
           FIT GQI+
Sbjct: 323 FITEGQIY 330
>NP_004037
          Length = 553

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   IFITSGQIF 9
           I IT GQIF
Sbjct: 386 ISITDGQIF 394
>NP_001001937
          Length = 553

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   IFITSGQIF 9
           I IT GQIF
Sbjct: 386 ISITDGQIF 394
>NP_001244264
          Length = 503

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   IFITSGQIF 9
           I IT GQIF
Sbjct: 336 ISITDGQIF 344
>NP_001001935
          Length = 503

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   IFITSGQIF 9
           I IT GQIF
Sbjct: 336 ISITDGQIF 344
>NP_001244263
          Length = 531

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   IFITSGQIF 9
           I IT GQIF
Sbjct: 364 ISITDGQIF 372
>NP_004688
          Length = 522

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IFITSGQIF 9
          I ITSG++F
Sbjct: 65 INITSGEVF 73
>NP_001231855
          Length = 521

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IFITSGQIF 9
          I ITSG++F
Sbjct: 64 INITSGEVF 72
>NP_063946
          Length = 780

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  IFITSGQI 8
          +FITSG I
Sbjct: 31 LFITSGTI 38
>NP_001137446
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  IFITSGQI 8
          +FITSG I
Sbjct: 31 LFITSGTI 38
>NP_001684
          Length = 511

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FITSGQIF 9
           +IT GQI+
Sbjct: 371 YITEGQIY 378
>NP_060352
          Length = 311

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   ITSGQIF 9
           +T GQIF
Sbjct: 225 VTEGQIF 231
>NP_001257880
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           FI+SGQ+
Sbjct: 216 FISSGQV 222
>NP_001257879
          Length = 337

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           FI+SGQ+
Sbjct: 254 FISSGQV 260
>NP_003830
          Length = 616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           FI+SGQ+
Sbjct: 533 FISSGQV 539
>NP_001265197
          Length = 602

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           FI+SGQ+
Sbjct: 519 FISSGQV 525
>NP_758424
          Length = 1642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    TSGQIF 9
            TSGQ+F
Sbjct: 1353 TSGQVF 1358
>NP_061142
          Length = 1642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    TSGQIF 9
            TSGQ+F
Sbjct: 1353 TSGQVF 1358
>NP_001265354
          Length = 1774

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    TSGQIF 9
            TSGQ+F
Sbjct: 1018 TSGQVF 1023
>NP_443124
          Length = 2459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    TSGQIF 9
            TSGQ+F
Sbjct: 1703 TSGQVF 1708
>NP_001177951
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2 FITSGQI 8
         F+TSG++
Sbjct: 3 FVTSGEL 9
>NP_006556
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 331 FVTSGEL 337
>NP_114409
          Length = 467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TSGQIF 9
           TSGQ+F
Sbjct: 404 TSGQLF 409
>NP_995322
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   IFITSGQI 8
           +FIT G I
Sbjct: 130 VFITKGDI 137
>NP_001255979
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 117 FVTSGEL 123
>NP_001255983
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  FITSGQI 8
          F+TSG++
Sbjct: 60 FVTSGEL 66
>NP_001255971
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_001255970
          Length = 663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_001255978
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 117 FVTSGEL 123
>NP_001255972
          Length = 700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_001255981
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_542185
          Length = 663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_001255976
          Length = 573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_001255975
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_001255974
          Length = 627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_001255973
          Length = 665

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_001255969
          Length = 663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_001255984
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  FITSGQI 8
          F+TSG++
Sbjct: 60 FVTSGEL 66
>NP_001255977
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_001255980
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 322 FVTSGEL 328
>NP_006412
          Length = 1849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FITSGQIF 9
            F+TS Q+F
Sbjct: 1673 FLTSQQLF 1680
>NP_001269869
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   ITSGQIF 9
           +TSG++F
Sbjct: 151 LTSGEVF 157
>NP_001034934
          Length = 518

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   ITSGQIF 9
           +TSG++F
Sbjct: 151 LTSGEVF 157
>NP_001032394
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   IFITSGQI 8
           IF+ +GQ+
Sbjct: 135 IFVNAGQV 142
>NP_001010861
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   IFITSGQI 8
           IF+ +GQ+
Sbjct: 135 IFVNAGQV 142
>NP_060223
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   IFITSGQI 8
           IF+ +GQ+
Sbjct: 140 IFVNAGQV 147
>XP_005255832
          Length = 725

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FITSGQI 8
           F+TSG++
Sbjct: 331 FVTSGEL 337
>XP_005254365
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   IFITSGQI 8
           +FIT G I
Sbjct: 130 VFITKGDI 137
>XP_006716481
          Length = 1649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FITSGQIF 9
            F+TS Q+F
Sbjct: 1473 FLTSQQLF 1480
>XP_005251193
          Length = 1800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FITSGQIF 9
            F+TS Q+F
Sbjct: 1673 FLTSQQLF 1680
>XP_005251192
          Length = 1843

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FITSGQIF 9
            F+TS Q+F
Sbjct: 1667 FLTSQQLF 1674
>XP_005251191
          Length = 1849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FITSGQIF 9
            F+TS Q+F
Sbjct: 1673 FLTSQQLF 1680
>XP_005246710
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  IFITSGQIF 9
          +F+ +G+IF
Sbjct: 72 VFLWNGEIF 80
>XP_005246372
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  ITSGQIF 9
          +TSG++F
Sbjct: 53 LTSGEVF 59
>NP_061921
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  IFITSGQIF 9
          +F+ +G+IF
Sbjct: 72 VFLWNGEIF 80
>NP_997182
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   IFITSGQI 8
           IF+ +GQ+
Sbjct: 140 IFVNAGQV 147
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.159    0.456 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 485,211
Number of extensions: 711
Number of successful extensions: 59
Number of sequences better than 10000.0: 59
Number of HSP's gapped: 59
Number of HSP's successfully gapped: 59
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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