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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     390
XP_006713721                                                           20     390
NP_002477                                                              17    3301
XP_005252054                                                           17    3301
NP_003801                                                              17    4312
NP_056312                                                              16    7355
NP_001273416                                                           16    7355
XP_006720531                                                           16    7355
XP_005254344                                                           16    7355

>NP_006209
          Length = 1068

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  IFISVTQEA 9
          IF+SVTQEA
Sbjct: 69 IFVSVTQEA 77



>XP_006713721
          Length = 1068

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  IFISVTQEA 9
          IF+SVTQEA
Sbjct: 69 IFVSVTQEA 77



>NP_002477
          Length = 790

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FISVTQE 8
           F+SVTQE
Sbjct: 152 FVSVTQE 158



>XP_005252054
          Length = 723

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  FISVTQE 8
          F+SVTQE
Sbjct: 85 FVSVTQE 91



>NP_003801
          Length = 281

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IFISVTQE 8
           IF+SVT E
Sbjct: 256 IFVSVTNE 263



>NP_056312
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IFISVTQ 7
           IFIS+TQ
Sbjct: 378 IFISLTQ 384



>NP_001273416
          Length = 494

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IFISVTQ 7
           IFIS+TQ
Sbjct: 317 IFISLTQ 323



>XP_006720531
          Length = 415

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IFISVTQ 7
           IFIS+TQ
Sbjct: 238 IFISLTQ 244



>XP_005254344
          Length = 556

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IFISVTQ 7
           IFIS+TQ
Sbjct: 379 IFISLTQ 385



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.133    0.320 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 347,788
Number of extensions: 526
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)