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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              19     869
NP_542178                                                              16    5631
NP_004056                                                              16    5631
NP_620168                                                              16    7355
NP_001171473                                                           16    7355
XP_006722720                                                           16    7355
XP_006722719                                                           16    7355
XP_006722718                                                           16    7355
XP_006722717                                                           16    7355
NP_963844                                                              16    7355
NP_005236                                                              15    9605
XP_006714202                                                           15    9605
XP_005262892                                                           15    9605
XP_005262891                                                           15    9605

>NP_000312
          Length = 928

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IFIEAVDLD 9
           IFI AVDLD
Sbjct: 103 IFIAAVDLD 111



>NP_542178
          Length = 235

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FIEAVDLD 9
          FI+A DLD
Sbjct: 17 FIDAKDLD 24



>NP_004056
          Length = 262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FIEAVDL 8
           F+EA+DL
Sbjct: 111 FLEAIDL 117



>NP_620168
          Length = 1300

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   FIEAVDLD 9
           ++E VD+D
Sbjct: 266 YVETVDID 273



>NP_001171473
          Length = 1263

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   FIEAVDLD 9
           ++E VD+D
Sbjct: 213 YVETVDID 220



>XP_006722720
          Length = 820

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   FIEAVDLD 9
           ++E VD+D
Sbjct: 266 YVETVDID 273



>XP_006722719
          Length = 1224

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   FIEAVDLD 9
           ++E VD+D
Sbjct: 180 YVETVDID 187



>XP_006722718
          Length = 1310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   FIEAVDLD 9
           ++E VD+D
Sbjct: 266 YVETVDID 273



>XP_006722717
          Length = 1310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   FIEAVDLD 9
           ++E VD+D
Sbjct: 266 YVETVDID 273



>NP_963844
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   FIEAVDLD 9
           F+E +D+D
Sbjct: 191 FLEVIDVD 198



>NP_005236
          Length = 4588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IFIEAVDLD 9
           IF+ + DLD
Sbjct: 737 IFMNSTDLD 745



>XP_006714202
          Length = 4588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IFIEAVDLD 9
           IF+ + DLD
Sbjct: 737 IFMNSTDLD 745



>XP_005262892
          Length = 4600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IFIEAVDLD 9
           IF+ + DLD
Sbjct: 737 IFMNSTDLD 745



>XP_005262891
          Length = 4600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IFIEAVDLD 9
           IF+ + DLD
Sbjct: 737 IFMNSTDLD 745



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.158    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 417,821
Number of extensions: 798
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)