Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           20     509
NP_000170                                                              20     509
NP_001268422                                                           20     509
NP_001268423                                                           20     509
XP_005264328                                                           20     509
NP_001137295                                                           17    4312
NP_001230633                                                           17    4312
NP_003400                                                              17    4312
NP_001230631                                                           17    4312
NP_056508                                                              16    5631
NP_065930                                                              16    5631
NP_001004477                                                           16    5631
NP_002408                                                              16    7355
NP_001139438                                                           16    7355
NP_001277131                                                           16    7355
NP_849191                                                              16    7355
NP_001092105                                                           16    7355
XP_006717927                                                           16    7355
NP_001136277                                                           15    9605
NP_005620                                                              15    9605
NP_115495                                                              15    9605
NP_001229342                                                           15    9605
NP_005519                                                              15    9605
NP_055043                                                              15    9605
NP_005236                                                              15    9605
NP_001020016                                                           15    9605
NP_001139807                                                           15    9605
NP_060527                                                              15    9605
NP_877499                                                              15    9605
NP_964012                                                              15    9605
NP_008865                                                              15    9605
NP_001248309                                                           15    9605
NP_056240                                                              15    9605
NP_006248                                                              15    9605
NP_001269573                                                           15    9605
XP_006718593                                                           15    9605
XP_006718592                                                           15    9605
XP_005252554                                                           15    9605
XP_006717528                                                           15    9605
XP_005252553                                                           15    9605
XP_006714202                                                           15    9605
XP_005262892                                                           15    9605
XP_005262891                                                           15    9605
XP_006712046                                                           15    9605
XP_006710903                                                           15    9605
XP_006710057                                                           15    9605
>NP_001268421
          Length = 1230

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    FFVELSET 9
            FFVELSET
Sbjct: 1060 FFVELSET 1067
>NP_000170
          Length = 1360

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    FFVELSET 9
            FFVELSET
Sbjct: 1190 FFVELSET 1197
>NP_001268422
          Length = 1058

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   FFVELSET 9
           FFVELSET
Sbjct: 888 FFVELSET 895
>NP_001268423
          Length = 1058

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   FFVELSET 9
           FFVELSET
Sbjct: 888 FFVELSET 895
>XP_005264328
          Length = 1261

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    FFVELSET 9
            FFVELSET
Sbjct: 1091 FFVELSET 1098
>NP_001137295
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FFVELSET 9
          FF+ +SET
Sbjct: 3  FFISMSET 10
>NP_001230633
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FFVELSET 9
          FF+ +SET
Sbjct: 3  FFISMSET 10
>NP_003400
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FFVELSET 9
          FF+ +SET
Sbjct: 3  FFISMSET 10
>NP_001230631
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FFVELSET 9
          FF+ +SET
Sbjct: 3  FFISMSET 10
>NP_056508
          Length = 942

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  IFFVELSE 8
          +F++ELSE
Sbjct: 65 LFYLELSE 72
>NP_065930
          Length = 955

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  FVELSET 9
          FV+LSET
Sbjct: 38 FVDLSET 44
>NP_001004477
          Length = 326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FFVELSET 9
           FF+ L+ET
Sbjct: 169 FFISLTET 176
>NP_002408
          Length = 3256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FVELSET 9
            F ELSET
Sbjct: 2682 FTELSET 2688
>NP_001139438
          Length = 2896

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FVELSET 9
            F ELSET
Sbjct: 2322 FTELSET 2328
>NP_001277131
          Length = 869

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FFVELS 7
           FFVELS
Sbjct: 380 FFVELS 385
>NP_849191
          Length = 994

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FFVELS 7
           FFVELS
Sbjct: 505 FFVELS 510
>NP_001092105
          Length = 993

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FFVELS 7
           FFVELS
Sbjct: 505 FFVELS 510
>XP_006717927
          Length = 2406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FVELSET 9
            F ELSET
Sbjct: 1832 FTELSET 1838
>NP_001136277
          Length = 625

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IFFVELS 7
           IFF+E+S
Sbjct: 105 IFFLEIS 111
>NP_005620
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IFFVELS 7
           IFF+E+S
Sbjct: 105 IFFLEIS 111
>NP_115495
          Length = 6306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    FFVELSE 8
            FFVEL E
Sbjct: 5464 FFVELYE 5470
>NP_001229342
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   FFVELSET 9
           +F+E+S+T
Sbjct: 121 YFLEMSDT 128
>NP_005519
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FVELSE 8
          FVELSE
Sbjct: 76 FVELSE 81
>NP_055043
          Length = 636

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IFFVELS 7
          +FF+ELS
Sbjct: 90 LFFLELS 96
>NP_005236
          Length = 4588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FFVELSET 9
            + VELSET
Sbjct: 1672 YSVELSET 1679
>NP_001020016
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IFFVELS 7
          +FF+ELS
Sbjct: 80 LFFMELS 86
>NP_001139807
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IFFVELS 7
          +FF+ELS
Sbjct: 7  LFFLELS 13
>NP_060527
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IFFVELS 7
           +FF+ELS
Sbjct: 114 LFFLELS 120
>NP_877499
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IFFVELS 7
           +FF+ELS
Sbjct: 114 LFFLELS 120
>NP_964012
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IFFVELS 7
           +FF+ELS
Sbjct: 153 LFFMELS 159
>NP_008865
          Length = 652

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IFFVELS 7
           +FF+ELS
Sbjct: 99  LFFMELS 105
>NP_001248309
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IFFVELS 7
          +FF+ELS
Sbjct: 84 LFFMELS 90
>NP_056240
          Length = 1720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    IFFVELSET 9
            I+F  +SET
Sbjct: 1154 IYFASVSET 1162
>NP_006248
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   FFVELSET 9
           +F+E+S+T
Sbjct: 121 YFLEMSDT 128
>NP_001269573
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  FFVELSET 9
          +F+E+S+T
Sbjct: 85 YFLEMSDT 92
>XP_006718593
          Length = 242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FVELSE 8
          FVELSE
Sbjct: 76 FVELSE 81
>XP_006718592
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FVELSE 8
          FVELSE
Sbjct: 76 FVELSE 81
>XP_005252554
          Length = 697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   FFVELSET 9
           +F+E+S+T
Sbjct: 155 YFLEMSDT 162
>XP_006717528
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   FFVELSET 9
           +F+E+S+T
Sbjct: 121 YFLEMSDT 128
>XP_005252553
          Length = 740

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   FFVELSET 9
           +F+E+S+T
Sbjct: 155 YFLEMSDT 162
>XP_006714202
          Length = 4588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FFVELSET 9
            + VELSET
Sbjct: 1672 YSVELSET 1679
>XP_005262892
          Length = 4600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FFVELSET 9
            + VELSET
Sbjct: 1672 YSVELSET 1679
>XP_005262891
          Length = 4600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FFVELSET 9
            + VELSET
Sbjct: 1672 YSVELSET 1679
>XP_006712046
          Length = 1612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    IFFVELSET 9
            I+F  +SET
Sbjct: 1046 IYFASVSET 1054
>XP_006710903
          Length = 649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IFFVELS 7
           +FF+ELS
Sbjct: 153 LFFMELS 159
>XP_006710057
          Length = 99

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IFFVELS 7
          IFF+E+S
Sbjct: 47 IFFLEIS 53
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.146    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 333,853
Number of extensions: 339
Number of successful extensions: 46
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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