Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_789765                                                              19     665
NP_001123914                                                           19     665
NP_005334                                                              19     665
NP_004976                                                              19     665
NP_203524                                                              19     665
NP_002515                                                              19     665
XP_006725199                                                           19     665
XP_006719132                                                           19     665
XP_006718280                                                           19     665
NP_853510                                                              19     869
NP_060680                                                              17    2528
NP_005393                                                              17    3301
NP_002872                                                              17    3301
NP_001361                                                              17    3301
XP_006715826                                                           17    3301
XP_006715825                                                           17    3301
XP_005263786                                                           17    3301
XP_005263785                                                           17    3301
XP_005263784                                                           17    3301
XP_005263781                                                           17    3301
XP_006712019                                                           17    3301
NP_060918                                                              17    3301
NP_001002858                                                           16    5631
NP_001129487                                                           16    5631
NP_004030                                                              16    5631
NP_001002857                                                           16    5631
NP_006816                                                              15    9605
NP_057427                                                              15    9605
NP_001138501                                                           15    9605
NP_064502                                                              15    9605
XP_006722388                                                           15    9605

>NP_789765
          Length = 170

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150



>NP_001123914
          Length = 189

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150



>NP_005334
          Length = 189

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150



>NP_004976
          Length = 188

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150



>NP_203524
          Length = 189

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150



>NP_002515
          Length = 189

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150



>XP_006725199
          Length = 170

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150



>XP_006719132
          Length = 189

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150



>XP_006718280
          Length = 170

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150



>NP_853510
          Length = 233

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           +ETS KTRQ
Sbjct: 178 VETSAKTRQ 186



>NP_060680
          Length = 390

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   IETSVKTR 8
           IET VKTR
Sbjct: 312 IETGVKTR 319



>NP_005393
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSVKTR 8
           +ETS KTR
Sbjct: 154 VETSAKTR 161



>NP_002872
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSVKTR 8
           +ETS KTR
Sbjct: 155 VETSAKTR 162



>NP_001361
          Length = 4158

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    IETSVKT 7
            IETSVKT
Sbjct: 3330 IETSVKT 3336



>XP_006715826
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSVKTR 8
           +ETS KTR
Sbjct: 154 VETSAKTR 161



>XP_006715825
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSVKTR 8
           +ETS KTR
Sbjct: 154 VETSAKTR 161



>XP_005263786
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSVKTR 8
           +ETS KTR
Sbjct: 155 VETSAKTR 162



>XP_005263785
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSVKTR 8
           +ETS KTR
Sbjct: 155 VETSAKTR 162



>XP_005263784
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSVKTR 8
           +ETS KTR
Sbjct: 155 VETSAKTR 162



>XP_005263781
          Length = 228

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSVKTR 8
           +ETS KTR
Sbjct: 177 VETSAKTR 184



>XP_006712019
          Length = 4158

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    IETSVKT 7
            IETSVKT
Sbjct: 3330 IETSVKT 3336



>NP_060918
          Length = 1230

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ETSVKTRQ 9
           E SVKTRQ
Sbjct: 445 EKSVKTRQ 452



>NP_001002858
          Length = 357

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  IETSVKTR 8
          IET++KT+
Sbjct: 60 IETAIKTK 67



>NP_001129487
          Length = 339

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  IETSVKTR 8
          IET++KT+
Sbjct: 42 IETAIKTK 49



>NP_004030
          Length = 339

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  IETSVKTR 8
          IET++KT+
Sbjct: 42 IETAIKTK 49



>NP_001002857
          Length = 339

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  IETSVKTR 8
          IET++KT+
Sbjct: 42 IETAIKTK 49



>NP_006816
          Length = 602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           I+TS K+R+
Sbjct: 296 IQTSAKSRE 304



>NP_057427
          Length = 3114

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IETSVKTRQ 9
           +ET +KT+Q
Sbjct: 647 LETCLKTQQ 655



>NP_001138501
          Length = 1392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ETSVKT 7
           ETSVKT
Sbjct: 367 ETSVKT 372



>NP_064502
          Length = 1176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ETSVKT 7
           ETSVKT
Sbjct: 126 ETSVKT 131



>XP_006722388
          Length = 1511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ETSVKT 7
           ETSVKT
Sbjct: 367 ETSVKT 372



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.113    0.258 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 290,572
Number of extensions: 270
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)