Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_789765                                                              19     665
NP_001123914                                                           19     665
NP_005334                                                              19     665
NP_004976                                                              19     665
NP_203524                                                              19     665
NP_002515                                                              19     665
XP_006725199                                                           19     665
XP_006719132                                                           19     665
XP_006718280                                                           19     665
NP_853510                                                              19     869
NP_005393                                                              17    3301
NP_002872                                                              17    3301
XP_006715826                                                           17    3301
XP_006715825                                                           17    3301
XP_005263786                                                           17    3301
XP_005263785                                                           17    3301
XP_005263784                                                           17    3301
XP_005263781                                                           17    3301
XP_005245610                                                           17    4312
XP_006711653                                                           17    4312
XP_006711652                                                           17    4312
XP_006711651                                                           17    4312
XP_006711650                                                           17    4312
XP_006711649                                                           17    4312
XP_006711648                                                           17    4312
XP_006711647                                                           17    4312
NP_055868                                                              17    4312
NP_036586                                                              16    5631
NP_005582                                                              16    7355
NP_078860                                                              16    7355
NP_071896                                                              16    7355
NP_060680                                                              16    7355
XP_005274065                                                           16    7355
XP_006718905                                                           16    7355
XP_005274064                                                           16    7355
XP_005270105                                                           16    7355
XP_005248564                                                           16    7355
XP_005264617                                                           16    7355
NP_001136155                                                           16    7355
NP_005900                                                              16    7355
NP_006816                                                              15    9605
NP_570137                                                              15    9605
NP_008841                                                              15    9605
NP_001026847                                                           15    9605
NP_061173                                                              15    9605
NP_079143                                                              15    9605
XP_005255519                                                           15    9605
XP_005255518                                                           15    9605
XP_006714564                                                           15    9605
XP_006714563                                                           15    9605
XP_005248434                                                           15    9605
XP_005248433                                                           15    9605
>NP_789765
          Length = 170

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150
>NP_001123914
          Length = 189

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150
>NP_005334
          Length = 189

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150
>NP_004976
          Length = 188

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150
>NP_203524
          Length = 189

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150
>NP_002515
          Length = 189

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150
>XP_006725199
          Length = 170

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150
>XP_006719132
          Length = 189

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150
>XP_006718280
          Length = 170

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           IETS KTRQ
Sbjct: 142 IETSAKTRQ 150
>NP_853510
          Length = 233

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           +ETS KTRQ
Sbjct: 178 VETSAKTRQ 186
>NP_005393
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSTKTR 8
           +ETS KTR
Sbjct: 154 VETSAKTR 161
>NP_002872
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSTKTR 8
           +ETS KTR
Sbjct: 155 VETSAKTR 162
>XP_006715826
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSTKTR 8
           +ETS KTR
Sbjct: 154 VETSAKTR 161
>XP_006715825
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSTKTR 8
           +ETS KTR
Sbjct: 154 VETSAKTR 161
>XP_005263786
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSTKTR 8
           +ETS KTR
Sbjct: 155 VETSAKTR 162
>XP_005263785
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSTKTR 8
           +ETS KTR
Sbjct: 155 VETSAKTR 162
>XP_005263784
          Length = 206

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSTKTR 8
           +ETS KTR
Sbjct: 155 VETSAKTR 162
>XP_005263781
          Length = 228

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IETSTKTR 8
           +ETS KTR
Sbjct: 177 VETSAKTR 184
>XP_005245610
          Length = 1207

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IETSTKT 7
           +ETSTKT
Sbjct: 943 LETSTKT 949
>XP_006711653
          Length = 801

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IETSTKT 7
           +ETSTKT
Sbjct: 437 LETSTKT 443
>XP_006711652
          Length = 1199

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IETSTKT 7
           +ETSTKT
Sbjct: 944 LETSTKT 950
>XP_006711651
          Length = 1208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IETSTKT 7
           +ETSTKT
Sbjct: 944 LETSTKT 950
>XP_006711650
          Length = 1299

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IETSTKT 7
           +ETSTKT
Sbjct: 944 LETSTKT 950
>XP_006711649
          Length = 1307

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IETSTKT 7
           +ETSTKT
Sbjct: 943 LETSTKT 949
>XP_006711648
          Length = 1308

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IETSTKT 7
           +ETSTKT
Sbjct: 944 LETSTKT 950
>XP_006711647
          Length = 1308

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IETSTKT 7
           +ETSTKT
Sbjct: 944 LETSTKT 950
>NP_055868
          Length = 701

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   IETSTKT 7
           +ETSTKT
Sbjct: 437 LETSTKT 443
>NP_036586
          Length = 1701

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           IETST+T +
Sbjct: 476 IETSTETAE 484
>NP_005582
          Length = 708

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   IETSTKTR 8
           +ETST++R
Sbjct: 599 LETSTRSR 606
>NP_078860
          Length = 278

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ETSTKTRQ 9
           ET++KTR+
Sbjct: 134 ETASKTRE 141
>NP_071896
          Length = 800

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ETSTKTRQ 9
           E STKTR+
Sbjct: 282 EKSTKTRK 289
>NP_060680
          Length = 390

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   IETSTKTR 8
           IET  KTR
Sbjct: 312 IETGVKTR 319
>XP_005274065
          Length = 552

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   IETSTKTR 8
           +ETST++R
Sbjct: 443 LETSTRSR 450
>XP_006718905
          Length = 707

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   IETSTKTR 8
           +ETST++R
Sbjct: 598 LETSTRSR 605
>XP_005274064
          Length = 708

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   IETSTKTR 8
           +ETST++R
Sbjct: 599 LETSTRSR 606
>XP_005270105
          Length = 500

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ETSTKTRQ 9
           E STKTR+
Sbjct: 282 EKSTKTRK 289
>XP_005248564
          Length = 2342

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    ETSTKT 7
            ETSTKT
Sbjct: 1900 ETSTKT 1905
>XP_005264617
          Length = 442

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ETSTKTRQ 9
           ET++KTR+
Sbjct: 298 ETASKTRE 305
>NP_001136155
          Length = 440

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ETSTKTRQ 9
           ET++KTR+
Sbjct: 296 ETASKTRE 303
>NP_005900
          Length = 2468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    ETSTKT 7
            ETSTKT
Sbjct: 2026 ETSTKT 2031
>NP_006816
          Length = 602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           I+TS K+R+
Sbjct: 296 IQTSAKSRE 304
>NP_570137
          Length = 203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ETSTKTRQ 9
           ETS+KT Q
Sbjct: 152 ETSSKTGQ 159
>NP_008841
          Length = 1792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           +ETS K+R+
Sbjct: 954 LETSRKSRE 962
>NP_001026847
          Length = 203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ETSTKTRQ 9
           ETS+KT Q
Sbjct: 152 ETSSKTGQ 159
>NP_061173
          Length = 1758

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           +ETS K+R+
Sbjct: 920 LETSRKSRE 928
>NP_079143
          Length = 1822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    IETSTK 6
            IETSTK
Sbjct: 1418 IETSTK 1423
>XP_005255519
          Length = 1796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IETSTKTRQ 9
           +ETS K+R+
Sbjct: 958 LETSRKSRE 966
>XP_005255518
          Length = 1830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IETSTKTRQ 9
            +ETS K+R+
Sbjct: 992  LETSRKSRE 1000
>XP_006714564
          Length = 1537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    IETSTK 6
            IETSTK
Sbjct: 1418 IETSTK 1423
>XP_006714563
          Length = 1766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    IETSTK 6
            IETSTK
Sbjct: 1361 IETSTK 1366
>XP_005248434
          Length = 1818

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    IETSTK 6
            IETSTK
Sbjct: 1413 IETSTK 1418
>XP_005248433
          Length = 1823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    IETSTK 6
            IETSTK
Sbjct: 1418 IETSTK 1423
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.105    0.239 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 342,143
Number of extensions: 442
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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